Showing papers in "Journal of Lipid Research in 1978"

TL;DR: Determination of the extent of the unprecipitated apoB-associated lipoproteins by sensitive radioimmunoassay and of the amount of precipitated high density lipoprotein by radial immunodiffusion assay of apolipoproteins A-I and A-II at various heparin and Mn(2+) concentrations indicated that the usual Heparin level was adequate.

TL;DR: The resulting tables provide precise values for the maximal amount of cholesterol that would be soluble in bile at any total lipid concentration and bile salt-lecithin ratio and allow for rapid and accurate calculation of lithogenic index or percent cholesterol saturation from the moles percent cholesterol actually present in hepatic, gallbladder, and duodenal biles.

TL;DR: Procedures for the determination of free and total cholesterol in lipid extracts or sonicates of 10(4) cultured human skin fibroblasts are described, permitting one person to carry out analyses of 30 or more subcultures in one day.

TL;DR: It was established that the bile acid concentration in bile samples with a high molar percentage of cholesterol would be overestimated if 3 beta-hydroxysteroid dehydrogenase were present with the 3 alpha-enzyme.

TL;DR: It is rapid, in that free cholesterol can be read in 5 minutes and total cholesterol after 20 minutes, and the precision of the method is greater than that obtained from gas-liquid chromatography.

TL;DR: In the early stages of peroxidation of linoleic acid, thiobarbituric acid value, the amount of conjugated diene, oxygen consumption, and peroxide value were in parallel with one another.

TL;DR: The functional interrelationships of the lipoprotein apoproteins and the information these relationships reveal about the mechanisms and determinants involved in thelipoprotein metabolism within the vascular bed are focused on.

TL;DR: The binding of polyphosphoinositides to the antibiotic neomycin is utilized for the purification of these lipids and the lipid is completely eluted by the addition of either ammonia or HC1 to the solvent.

TL;DR: It was concluded that cells from the epididymal fat pad from small rats can be isolated in a homogenous fraction that develops in culture into cells of identical morphology and function as adipocytes formed in vivo.

TL;DR: The kinetics of apolipoproteins B and C were studied in 14 normal and hyperlipoproteinemic subjects after injection of exogenously-labeled very low density lipoprotein (VLDL) particles, finding a slowdown of the stepwise delipidation process in all hyperlipemic individuals studied.

TL;DR: Content of apoprotein A-I was not altered appreciably by exposure to serum or its ultracentrifugal fractions, whereas content of C apoproteins, estimated from intensity of staining of the low molecular weight protein component in polyacrylamide gel electropherograms, increased in all cases except for the density fraction greater than 1.21 g/ml.

TL;DR: Gangliosides were isolated from whole spinal cords and cord myelin of human, cat, and rabbit by a revised methodology and the most prominent and consistent features were the reduced concentration of Gd1a and increased amounts of Gm3 and Gd3.

TL;DR: It is concluded that the present experimental conditions that primarily influence cholesterol and bile acid biosynthesis also affect the metabolism of plasma triglycerides-and presumably that of very low density lipoprotein-in a regulatory manner.

TL;DR: A new method has been developed for measurement of cholesterol absorption that offers the advantages of simplicity of administration of isotope, ease of analysis of stool samples, and daily repeatability of analysis.

TL;DR: Lysosomal storage diseases are caused by a genetic deficiency of specific acid hydrolases in lysosomes, and reversal of such enzyme deficiencies has been approached by means of direct enzyme replacement.

TL;DR: Serum and liver lipid levels increased with age in both strains but the liver phospholipid level remained unchanged, and the proportion of serum alpha-lipoprotein increases and that of beta- and prebeta- lipoproteins slightly decreased.

TL;DR: The techniques described allow the quantitation of cholesterol, cerebrosides, sulfatides, ethanolamine phospholipids, phosphatidylcholine, -serine, -inositol, and gangliosides on 500 micrograms of lipid to study regional differences in the central nervous system, to analyze cell membranes or subcellular particles, or to analyze pathological biopsies in thecentral and peripheral nervous systems.

TL;DR: The osmic acid fixation-Coulter electronic counter method described for determining adipose cell size and number in intact adipose tissue fragments has been modified for use with suspensions of isolated rat and human adipose cells.

TL;DR: The presence of a high activity of phosphatidate phosphohydrolase in lung endoplasmic reticulum is consistent with the rapid synthesis of pulmonary surfactant phosphatidylcholine.

TL;DR: The high levels of free fatty acid and diglyceride reported in the cardiac lipids of rats fed rapeseed oil suggest that extensive autolysis occurs during homogenization with a rotating blade-type homogenizer.

TL;DR: It is concluded that both the intestine and liver are significant sources of apop protein A-I whereas only the liver synthesizes significant amounts of the arginine-rich apoprotein.

TL;DR: A column chromatographic procedure for the isolation and purification of GM3 from human liver, an important precursor in the synthesis of the more complex gangliosides, is devised.

TL;DR: ApoC-II and apoC-III of human very low density lipoproteins (VLDL) have been quantified by analytical isoelectric focusing (IEF) between pH 4 and 6 in polyacrylamide gels containing 8 M urea.

TL;DR: A simple mehtod using reverse-phase liquid chromatography is presented for resolution and quantitation of the major conjugated bile acid of man, including the glycine and taurine conjugates of the dihydroxy bile acids, chenodeoxycholic and deoxycholics acid.

TL;DR: The data indicate that the lipid composition of a lipoprotein can regulate free cholesterol uptake and esterification as well as cellular cholesterol content.

TL;DR: Feeding the liver donors a diet containing 1% cholesterol or 4% cholesterol and 1% cholic acid failed to alter the hepatic removal rate and the cholesteryl ester removed from the remnants was hydrolyzed at a rate that was a small fraction of the removal rate.

TL;DR: The enzymatic and physical-chemical characteristics of this soluble enzyme were remarkably similar to a recently described phospholipase A(2) of rabbit polymorphonuclear leukocytes derived from glycogen-induced peritoneal exudates.

TL;DR: It is suggested that an increased formation of plasma triglycerides--monitoring very low density lipoprotein synthesis--is linked to an enhanced degradation of cholesterol to bile acids and that there is an integrated regulation of the metabolism of these two parameters.

TL;DR: The characteristics of dietary cholesterol-induced hyperlipoproteinemia were studied in adult Macaca fascicularis to determine if significant male--female differences could be identified and chemical composition differences were also noted.