Showing papers in "Journal of Lipid Research in 1986"

TL;DR: This one-step direct transesterification procedure carried out in methanol-benzene 4:1 with acetyl chloride is superior to currently used methods not onlyBecause of its simplicity and speed, but also because of its added precision.

TL;DR: The data suggest that RBP is synthesized in many extrahepatic tissues and it is possible that this extra-hepatically synthesized RBP may function in the recycling of retinol from these tissues back to the liver or to other target organs.

TL;DR: Results indicate that apo[a] is not a superglycosylated form of apoB but is distinctly different in its composition and structure.

TL;DR: The timing and magnitude of metabolic adaptations in adipose tissue during lactogenesis and lactation were determined in first lactation bovines using in vitro rates of lipogenesis and palmitate esterification to estimate in vivo synthesis.

TL;DR: An accurate assay of the rate-limiting enzyme in bile acid synthesis, the cholesterol 7 alpha-hydroxylase, in human liver is described, based on isotope dilution-mass spectrometry, and endogenous microsomal cholesterol is used as the only substrate for the enzyme.

TL;DR: Large triglyceride-rich very low density lipoproteins from hypertriglyceridemic patients, but not VLDL from normal subjects, bind to the LDL receptor of human skin fibroblasts because they contain apolipoprotein E (apoE) of the correct conformation, accessible both to the HDL receptor and to specific proteolysis by alpha-thrombin.

TL;DR: It was found that the 3 alpha epimers of the five bile acids listed above enzymatically formed 3-O-glucuronides, C24 being the best substrate, followed by C21 and C20; C22 and C23 gave rise to only small amounts of this product.

TL;DR: In the normal animal, the primary function of receptor-dependent LDL transport is to promote the rapid uptake and disposal of plasma LDL by the liver, so substantial and predictable elevations in the circulating plasma LDL-cholesterol levels are required to maintain this balance.

TL;DR: The ethinyl estradiol-induced lipoprotein receptor of rat liver is biochemically and immunologically similar to the LDL receptor of other species and could account for LDL as well as beta VLDL and HDLc removal.

TL;DR: Lipid analysis of hepatic and intestinal microsomes demonstrated an enrichment of n-3 polyunsaturated fatty acids in membranes from rabbits ingesting the menhaden oil.

TL;DR: The utility of HPLC for analysis of molecular species of phospholipids is illustrated in studies in which it has been demonstrated that molecular species are selectively synthesized, selectively transported, and selectively participate in enzymatic reactions.

TL;DR: A theoretical analysis based on the experimental data suggests that very large lipophorin particles result from intermolecular fusion of the lipoph orin molecules that are activated by AKH.

TL;DR: With [3H-24,25]-dihydrolanosterol as substrate, large-scale metabolic formation of intermediates of lanosterol demethylation was carried out to identify all compounds in the metabolic process and characterized by ultraviolet, 1H-NMR, and infrared spectroscopy of the isolated sterols.

TL;DR: The application of the HPLC technique to environmental samples may provide an accurate assessment of archaebacterial biomass in various microbial communities.

TL;DR: It was found that human neutrophils contain approximately 300 pg/10(6) cells which increased 2-3-fold during the 5-min period following challenge with 1.9 microM calcium ionophore, A23187.

TL;DR: The present studies suggest that changes in the composition of mesenteric lymph triglyceride-rich lipoproteins may be of physiologic significance, perhaps relating to the potential atherogenicity of saturated lipids.

TL;DR: Measurement of acyltransferase activities in microsomes using 1- and 2-monoacyl- and monoalkylglycerols as substrates indicated that the monoacylglycerol acyl transferases from suckling liver and intestinal mucosa have different substrate specificities.

TL;DR: The effect of alloxan-induced insulin deficiency on high density lipoprotein (HDL) metabolism was studied in rabbits and data support the view that insulin plays an important role in regulation of HDL metabolism.

TL;DR: This chapter discusses several examples describing the use of continuous development (CD-HPTLC) for increasing the separation of several types of glycosphingolipids.

TL;DR: A novel approach to the degradation of lithocholic Acid acetate to 24-norlithocholic acid is described, which involves the photochemical modification of a Hunsdiecker reaction and Kornblum oxidation of the intermediate 23-bromide.

TL;DR: In this paper, factors regulating the concentrations of plasma low density lipoproteins (LDL), apolipoprotein B metabolism was studied in nine Pima Indians (25 +/- 2 yr, 191 +/- 20% ideal wt) with low LDL cholesterol and apoB (60 +/- 4 mg/dl) and in eight age-and weight-matched Caucasians with similar very lowdensity lipoprotein (VLDL) concentrations, but higher LDL (cholesterol = 104 +/- 18; apoprotein B = 82 +/- 10; P less than 0.05).

TL;DR: DG and other synthetic glycosides with similar activities may be of use in the management of hypercholesterolemia and atherosclerosis when long-term studies demonstrate that the glucoside is well tolerated.

TL;DR: The syntheses of several analogues of stearic acid in which oxygen or sulfur atoms or sulfoxide groups have replaced the methylene groups at either position 9 or position 10 of the polymethylene chains are reported.

TL;DR: It is concluded that cholesteryl ester storage in Tangier macrophages results from an imbalance of cholesterol influx and efflux, and the net increase of cholesterol caused by abnormal lipoproteins in certain postprandial states cannot be fully compensated by effective efflux and ultimately leads to macrophage cholesterel ester accumulation.

TL;DR: It is, therefore, now possible to analyze glycine-conjugated and unconjugated bile acids together on a single GLC column without the necessity for a hydrolytic step.

TL;DR: Vulpecholic acid was found in the bile in the unconjugated form; it accounted for more than 60% of the solid bile material and is the first example of a mammal secreting a 1 alpha-hydroxylated bile acid in a free form.

TL;DR: The extent of oxidoreduction of the 12 alpha-hydroxyl group in [12 beta-3H]-labeled deoxycholic acid given to two patients operated with subtotal colectomy and ileostomy was markedly reduced (less than 20% of normal).

TL;DR: The presence of a potent neutral-active sphingomyelinase in a plasma membrane-microsomal fraction of bovine adrenal medulla is demonstrated and may be involved in membrane fusion and lysis during catecholamine secretion through its ability to alter membrane composition.

TL;DR: A selected ion monitoring, gas-liquid chromatography-mass spectrometry micromethod which measures [2H3] tracer enrichment in 3-hydroxybutyrate and acetoacetate from 300-microliters blood samples is described.

TL;DR: It is suggested that this method can be used to detect the presence of LDL receptors on minute amounts of membrane Protein down to 3 micrograms of membrane protein.