Showing papers in "Journal of Microbiological Methods in 1991"

TL;DR: The results suggest the possibility that 16S rRNA genes of culturable and nonculturable Mollsicutes can be amplified for detection and for a phylogenetic study using crude Mollicutes DNA preparations under appropriately controlled thermocycling conditions.

TL;DR: In this article, the use of total lipid phosphate as a measure of biomass was evaluated in soils with different organic matter content, and the two digestion methods showed a good linear correlation (r2 = 0.991).

TL;DR: A sensitive rapid method was devised for screening bacterial colonies that produce surfactants by evaluating the stability of drops dependent on biosurfactant concentration and it correlated with surface tension but not with emulsifying activity.

TL;DR: In this article, the authors developed a computer-aided, fast-working procedure suitable for the identification of bacteria, which is based on spectral dissimilarity between spectra calculated as a suitably transformed and normalized Pearson's product moment correlation coefficient.

TL;DR: The multidish system proved to be far more suitable than the commercial kits in characterization of marine bacteria, both fish pathogenic and nonpathogen, and has applications both for numerical taxonomy and for diversity studies of bacterial communities.

TL;DR: Lactobacillus sake ATTC 15521 was sensitive to all of the bacteriocins and was chosen as the best indicator strain for further screening studies and gave a large number of false-negative results compared to the spot-on-the-lawn method.

TL;DR: In this paper, the biomass of Actinomadura madurae, Microtetraspora glauca and Planobispora rosea was hydrolyzed, the released sugars were reduced, acetylated and the resultant alditol acetates analysed by gas chromatography (GC) and GC-mass spectrometry.

TL;DR: Pre-adapting heterotrophic bacteria to the environment of the solid medium was found to improve both the plate efficiencies and the growth rates of iron-oxidizing acidophiles.

TL;DR: Findings indicate that the AO staining reaction may be suggestive of physiological activity under defined conditions, however, variables in staining and fixation procedures as well as uncertainties associated with mixed bacterial populations in environmental samples may produce results that are not consistent with the classical interpretation of this reaction.

TL;DR: The results obtained in this study showed that a kinetic method based on automated turbidometry provides a sophisticated tool for the determination of bacteriocin activity.

TL;DR: The most active and abundant phytotoxin formed by the plant parasitic fungus Bipolaris sorokiniana was prehelminthosporol (C15H22O2).

TL;DR: A new method using the chromogenic substrate 5-bromo-4-chloro-3-indolyl-α-d-galactoside was developed for the differential enumeration of Bifidobacterium spp.

TL;DR: Method ii proved to be the most effective procedure resulting in the highest isolation and lowest contamination rate and may be useful when testing water samples for Mycobacteria chelonae and M. fortuitum and if high concentrations of red pigmented contaminants resistant to CPC are to be expeected.

TL;DR: In this paper, the applicability of microbiological soil analyses for quantification of soil activity was investigated and the results showed that the Int method indicates a higher activity, ≈ 1.458-fold.

TL;DR: The results suggest that the 4-MUF assay is a sensitive, reliable, easily applied method for measuring chitinase activity in aquatic environments.

TL;DR: A rapid method has been developed to distinguish pure cultures of archaebacteria from eubacteria, and the height of the ester peak, expressed as a percent of the methyl peak, was chosen to distinguish the two kingdoms.

TL;DR: Investigations showed that 99% of C. albicans and all strains ofC.

TL;DR: The ability to analyse mRNA by this method has implications for the study of gene expression and the molecular mechanisms of pathogenicity in mycobacteria.

TL;DR: Water testing procedure described is based on the membrane filter technique and uses a non-selective enrichments period of 10 h at 37°C prior to the membranes being hybridised with the uidA gene probe, after which up to 500 faecal coliforms could be counted per membrane.

TL;DR: A method, which is generally applicable to a wide variety of methanogenic bacteria, for the extraction of restrictable genomic DNA by treatment of frozen-thawed cells with pronase, dithiothreitol and sodium dodecyl sulphate, followed by recovery of the DNA by ethanol precipitation.

TL;DR: In this paper, a modification of the most probable number (MPN) method for the determination of total viable microbial cells was investigated, which applied MPN statistics to microorganisms growing on solid instead of liquid medium which is used in the conventional method.

TL;DR: A screening method based in depth of clearing measurement of tributyrin medium opaque column is improved, which can be used as a presumptive screening of lipolytic fungi but can also give quantifiable results.

TL;DR: In this article, the potential for ion mobility spectrometry (IMS) for the detection and determination of living microorganisms was explored where a hand-held IMS instrument embodies the analytical concept and is an atmospheric pressure vapor detector.

TL;DR: In this article, a simple autoradiographic technique for the selective isolation of Cs-137-sorbing microorganisms from primary isolation plates was described. But the technique was not applied to the case of the Chernobyl nuclear accident.

TL;DR: T48 and T54 mutants were found to require cellobiose as an energy source for growth on minimal agar and exhibition of parental auxotrophic genotypes in the thermal environment, suggesting two stepwise mutational events can take place during a very prolonged exposure to thermal stress.

TL;DR: Data show that 31P NMR is a powerful tool for identifying and quantitating bacterial membrane lipids and provide a foundation for understanding the molecular interactions of these lipids with cationic antimicrobial peptides, such as defensins, magainins and polymyxin B.

TL;DR: This test is simple, inexpensive and rapid with the results being obtainable within ≈ 45–60 min after the receipt of the sera and has a potential for wider application in the serodiagnosis of hydatid disease in poorly equipped laboratories of developing countries.

TL;DR: Due to its high sensitivity with a lower limit of detectability of 1 ng·ml −1 of CPS, the test system may prove superior to standard chemical methods of CPS quantification.

TL;DR: Adhesin-coated FMPs are rapid and stable reagents for functional study of bacterial adhesion proteins and Binding tests on frozen sections of human kidney and with immobilized target proteins showed that the tissue- and molecular-binding specificities of the O75X-FMPs were closely similar to those of the intact O 75X adhesin.

TL;DR: Rates of acetylene reduction, determined with the third method, were lower in the deep sediment where few roots penetrated; over a 12-cm deep core, total rates were not significantly different from those obtained by the other methods.