Showing papers in "Journal of Microbiological Methods in 2007"

TL;DR: The hypervariable sequence-specific dendrograms and the "MEGALIGN" files provided online will be highly useful tools for designing specific probes and primers for molecular assays to detect pathogenic bacteria, including select agents.

TL;DR: A mutagenesis system based on the mobile group II intron from the ltrB gene of Lactococcus lactis to function in clostridial hosts is adapted, which is highly efficient and reproducible, and should revolutionize functional genomic studies inClostridia.

TL;DR: The popular CAS assay for siderophore detection, based on the utilization of chrome azurol S, was redesigned and optimized to produce a new, fast, non-toxic, and easy method to determine a wide variety of microorganisms capable of siderophile production on a solid medium.

TL;DR: A potential application of the novel chemical propidium monoazide (PMA), which results in the selective suppression of DNA detection from dead cells, in combination with quantitative PCR as a diagnostic tool, successfully monitored the disinfection efficacy of hypochlorite, benzalkonium and heat on several model pathogens.

TL;DR: Modifications to Nile Red are communicated that facilitate its use as a high-throughput screening technology, as well as improving its accuracy and versatility.

TL;DR: Bacterial communities of four arable soils were analysed by fingerprinting of 16S rRNA gene fragments amplified from total DNA of four replicate samples for each soil type, resulting in complex ribotype patterns.

TL;DR: The MLVA assay is rapid, highly discriminatory, and reproducible within human Brucella isolates, and can significantly contribute to epidemiological trace-back analysis of BrucellA infections and may advance surveillance and control of human brucellosis.

TL;DR: This study analyzed PCR specificity and efficiency of the ITS primers in a series of single- and mixed-template samples using a combined quantitative PCR-length heterogeneity analysis (LH-qPCR) approach and found variability associated with the individual steps of the LH- qPCR analysis was determined from environmental samples.

TL;DR: This work describes the implementation of a new assay named the BioFilm Ring Test to evaluate the ability of bacteria to form biofilms based on the immobilisation (or not) of magnetic beads embedded by bacterial aggregates or mats.

TL;DR: An automated incubation system for the study of gas kinetics in 15 parallel bacterial cultures is described, demonstrating the system's potential for unraveling contrasting patterns of denitrification gene expression as a function of concentrations of O(2) and NO in the medium.

TL;DR: The use of the multiplex PCR targeting inlA, inlC and inlJ genes facilitates simultaneous confirmation of L. monocytogenes species identity and virulence.

TL;DR: The results show that the efficacy of a disinfectant must be measured by using a laboratory method where biofilm is grown under fluid flow conditions similar to the environment where the disinfectant will be applied.

TL;DR: This study provides transferable staining and ESEM imaging protocols suitable for a wide range of biofilms, plus a novel tool for quantifying biofilm image data.

TL;DR: A multi-faceted approach resulted in substantial improvement in the microbiological detection and isolation of Bartonella when compared to direct inoculation of a blood agar plate, and may assist in the diagnostic confirmation of bartonellosis in dogs and other animals.

TL;DR: The findings indicate that this mLAMP method is a new convenient tool for simultaneous detection of the bovine Babesia parasites.

TL;DR: This study showed that while both XTT and viable count methods are comparable when estimating the overall antimicrobial activity of experimental substances, there is no strong linear correlation between the two methods.

TL;DR: It is shown that Salmonella isolates which have a rough lipopolysaccharide structure could be assigned to the serovar Enteritidis by the real-time PCR, and can contribute to meet the need of fast identification and detection methods for use in monitoring and control measures programmes.

TL;DR: The method is described and reports on the state-of-the-art application of this technique to the identification of microorganisms vehiculated with foods and beverages and the species important for food fermentation or deterioration will be discussed.

TL;DR: The findings suggest that MalariaCount can potentially be used as a tool to provide rapid and accurate determination of Parasitemia in research laboratories where frequent, large-scale, efficient determination of parasitemia is required.

TL;DR: In this paper, spectrophotometry, fluorescence and radioactivity techniques were used to characterize and quantify coaggregation of probiotic strains with pathogens, which were strain-specific and dependent on time and incubation conditions.

TL;DR: Real-time PCR technique is a sensitive and reliable technique, which can replace conventional PCR for clinical specimens with negative bacterial culture, and appears to be essential to perform in case of suspicion of septic arthritis.

TL;DR: It is shown that fungal ITS regions can be detected in RNA pools by RT-PCR amplification of fungal precursor rRNA molecules, which suggests that precursor r RNA molecules reside in the cells of active fungi for sufficient time to allow RT- PCR amplified of ITS regions prior to their removal by post-transcriptional cleavage.

TL;DR: A simple method to assay PO activity directly in bulk samples by spectrophotometric test using 2,2'-azinobis-(-3 ethylbenzothiazoline-6-sulfononic acid) diammonium salt (ABTS) as the substrate showed that PO attain their optimum activities when incubating 0.1 g of soil at 30 degrees C for 5 min.

TL;DR: Investigations suggest a preferential colonization of water rather than soil environments by P. aeruginosa, and a novel PCR screening, targeting the ecfX gene, which was developed and validated on environmental DNA extracts.

TL;DR: The transformed BIOLOG EcoPlates showed the ability of the logarithmic transform to reduce the influence of high leverage or outlying observations on the overall analysis and its robustness in terms of treating data from different ecological systems was illustrated.

TL;DR: Yeast transformation efficiency and expression cassette insertion were increased by constructing a strain containing a zeta docking platform for targeted integration into the genome and the coefficient of variance of the full process was reduced.

TL;DR: Standardized TRFLP is a robust, reproducible, and high-throughput method that will provide a useful biomarker for characterizing gut microbiota in human fecal samples and assessed the methodological and inter-individual variation in gut microbial community fingerprints.

TL;DR: For DGGE profiling of eubacteria within rumen samples, primers F968gc and R1401 proved the most specific and sensitive providing that touchdown PCR is not used.

TL;DR: It was experimentally established that the sensitivity of the magnetoelastic sensors was higher for sensors with smaller physical dimensions, and an increase in sensitivity from 159 Hz/decade for a 2 mm sensor to 770 Hz/century for a 1 mm sensor was observed.

TL;DR: A study of the relationship between the ECOR strains as determined by polymorphisms in seven variable-number of tandem repeats (VNTR) loci and the MLVA method was used to genotype isolates from patients and suspected sources in a recent outbreak of E. coli O103 in Norway.