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Showing papers in "Journal of Molecular Biology in 1973"


Journal ArticleDOI
TL;DR: Pulse-chase experiments in wild-type and mutant phage-infected cells provide evidence that the following particles called prohead I, II and III are successive precursors to the mature heads as discussed by the authors.

3,697 citations


Journal ArticleDOI
TL;DR: The RNAs and proteins specified by five early genes of bacteriophage T7 have been identified by electrophoresis on sodium dodecyl sulfate, polyacrylamide gels using a slab gel system in which 25 samples can be run simultaneously and then dried for autoradiography.

2,127 citations


Journal ArticleDOI
TL;DR: Temperature shift-down experiments, using several different phage carrying temperature-sensitive mutations in gene 21 , indicate that the bulk of the τ-particles cannot be used for normal phage production and strongly suggest that cleavage of the head proteins is required for DNA packaging to occur.

1,260 citations


Journal ArticleDOI
TL;DR: A computer program is described for calculating the environment and the exposure to solvent of atoms of a protein based on the atomic co-ordinates of the protein and on assumptions like those of Lee & Richards (1971).

1,158 citations


Journal ArticleDOI
TL;DR: The structure of lecithin-water phases observed below the melting temperature of the hydrocarbon chains, with special emphasis on the conformation of the chains, is described in this paper.

1,059 citations


Journal ArticleDOI
TL;DR: The occurrence of larger continuous folds (“super-secondary structures”) has been detected in the comparison of lactate dehydrogenase with itself and with other protein structures.

857 citations


Journal ArticleDOI
TL;DR: The C-protein molecule contains a single polypeptide chain of molecular weight 140,000 and the intrinsic viscosity of 13.6 ml/g suggests that the molecule is neither completely globular nor as elongated as molecules like paramyosin or tropomyosin.

582 citations


Journal ArticleDOI
TL;DR: The hypothesis that a RecF pathway of recombination can operate as a partially independent substitute for the RecBC pathway ofcombination is supported and there are partially independent RecF and RecBC pathways for the repair of u.v. damage.

469 citations


Journal ArticleDOI
TL;DR: A possible relationship between the smaller movement of the tropomyosin and the “catch” response of molluscan muscles is suggested and any increase of intensity on the 59 A and 51 A layer-lines is most likely to be associated with some extra mass attaching to the actin molecules.

469 citations


Journal ArticleDOI
TL;DR: In this paper, it was shown that β-pleated sheets with a right-hand twist when viewed along the polypeptide chain direction have a lower free energy than sheets that are straight or which have a left hand twist.

461 citations


Journal ArticleDOI
TL;DR: The kinetic data of inhibitor-enzyme interaction are reconciled with the structural model, and relations between enzyme-inhibitor interaction and productive enzyme-substrate interaction are proposed.

Journal ArticleDOI
TL;DR: In the proposed nomenclature restriction-modification systems are named according to host organism and strain, different R-M systems in a single host are designated by Roman numerals.

Journal ArticleDOI
TL;DR: It was found that interactions between amino acids of opposite charge and between large hydrophobic amino acids in the overlapping region between two chains are maximal when the chains are staggered by 0D, 1D, 2D, 3D and 4D, where D = 234 ± 1 residues.

Journal ArticleDOI
TL;DR: An estimate is made of the effect of imperfectly complementary sequences on the rate of reassociation of DNA, which appears to be reduced by a factor of two for each 10 deg.

Journal ArticleDOI
TL;DR: It is suggested that the proteins encoded by phage genes E, B and C , and the bacterial component defined by groE mutations act together at an early stage in head assembly.

Journal ArticleDOI
TL;DR: Major uncertainties and ambiguities in the search for and identification of true initiation factors, as opposed to structural ribosome proteins needed for the reconstitution of damaged ribosomes, can be reduced by using this system.

Journal ArticleDOI
TL;DR: A high degree of order exists in the arrangement of DNA sequences in the Xenopus genome, and a little more than 50% of the genome consists of closely interspersed repetitive and non-repetitive sequences.

Journal ArticleDOI
TL;DR: There is a small but significant difference in sedimentation distribution of new and old mRNA, the old mRNA sedimenting more slowly than new material, suggesting that the more stable species has a lower average molecular weight.

Journal ArticleDOI
TL;DR: It was found that approximately 44% of the F factor is homologous to several different fi+ R factors and to Col V-K94, and that all of the homology between these plasmids and F is restricted to a region comprising only one-half of theF-factor molecule.

Journal ArticleDOI
TL;DR: A physical map of the Simian virus 40 genome has been constructed on the basis of specific cleavage of Simianirus 40 DNA by bacterial restriction endonucleases and the single site in SV40 DNA cleaved by the Escherichia coli RI restrictions endonuclease has been located.

Journal ArticleDOI
TL;DR: It is concluded that an intact prohead structure is required to mature the virus DNA (i.e. to cut the overlength DNA concatemer to the mature length) and apparently this cutting occurs as part of the encapsulation event.

Journal ArticleDOI
TL;DR: Tables of temperature-dependent free energies that allow one to predict the stability and thermal transition temperature of many simple RNA secondary structures (applicable to ~1 m -Na + concentration).

Journal ArticleDOI
TL;DR: The molecular structure of the B form of DNA has been refined using the 3 A resolution X-ray diffraction intensities and supplementary stereochemical data and has no undesirable stereoehemical features, apparently as a result of having the furanose rings in a standard C3-exo conformation.

Journal ArticleDOI
TL;DR: It is proposed that the difference in regulation of these systems is that the interaction between myosin and actin is prevented by blocking sites on actin in the case of vertebrate muscles, whereas in the cases of molluscan muscles it is the sites on myOSin which are blocked in the absence of calcium.

Journal ArticleDOI
TL;DR: The number of copies of viralDNA in DNA of cells transformed by adenovirus type 2 has been determined by following the kinetics of reassociation of 32P-labeled viral DNA in the presence of unlabeled DNA extracted from transformed and control cells.

Journal ArticleDOI
TL;DR: It is proposed that the first step in the initiation of protein synthesis in the reticuloeyte lysate is the formation of a 40 S/Met-tRNA f complex, which binds mRNA at the correct initiation site and, after joining with a 60 S subunit, an 80 S /mRNA initiation complex is formed.

Journal ArticleDOI
TL;DR: Equatorial X-ray diffraction patterns have been studied from muscles at rest, during contraction and in rigor and it is confirmed that the relative intensity of the two main equatorial reflections depends both on the sarcomere length and on the state of the muscle.

Journal ArticleDOI
TL;DR: The stereo-chemical constraints on the transport of mineral ions into and within collagen fibrils of bone and tendon support the postulate that bone collagen is an in vivo catalyst for mineral deposition and further suggests that its catalytic activity may be partially regulated through its molecular packing.

Journal ArticleDOI
TL;DR: A way to join naturally occurring DNA molecules, independent of their base sequence, is proposed, based upon the presumed ability of the calf thymus enzyme terminal deoxynucleotidyltransferase to add homopolymer blocks to the ends of double-stranded DNA.

Journal ArticleDOI
TL;DR: It is concluded that concatemer formation, via replication or recombination, is an essential step in phage development.