Showing papers in "Journal of Molecular Biology in 1976"

TL;DR: Electrostatic stabilization is an important factor in increasing the rate of the reaction step that leads to the formation of the carbonium ion intermediate, found in the cleavage of a glycosidic bond by lysozyme.

TL;DR: Fusions of lac genes to selected locations on the Escherichia coli chromosome are useful in discovering new types of regulation of gene expression, as was found in the case of the araC gene.

TL;DR: The accessible surface areas have been calculated for the individual residues in 12 proteins, and for the extended chains, the secondary structures and tertiary structure of six proteins and it is shown that the accessible surface area of folded proteins is simply proportional to the two-thirds power of their molecular weight.

TL;DR: This work shows how the concept of time-averaged forces can be used to simplify conformafional energy calculations on globular proteins and shows that the simplifications work well in representing the stable native conformation of this globular protein.

TL;DR: The dominant factor driving complex formation between these charged ligands and the nucleic acid is the entropic contribution from the release of counterions, which appears to drive the non-specific interactions of proteins with nucleic acids.

TL;DR: It is found that the net result of four association and four dissociation steps is a lengthening of the filament by one protomer at one end and a corresponding shortening at the other.

TL;DR: The crystal structure analysis of horse liver alcohol dehydrogenase has been extended to 2.4 A resolution and the positions of the 374 amino acids in the polypeptide chain of each subunit are determined.

TL;DR: Lamellar X-ray diffraction from hydrated multilayers of a 3:2 molar mixture of egg lecithin and cholesterol has been analysed and molecular models of the bilayer are presented.

TL;DR: The properties of high reactivity under mild conditions, long solution half-life, and the radioactive label make [35S]dithiobis(succinimidyl propionate) a particularly useful and versatile probe of extended structures in a variety of biological systems.

TL;DR: A model for replication repair based on the process of branch migration explains the transient production of doubly substituted DNA within the first generation of incubation in bromodeoxyuridine and the appearance of four-pronged replication forks.

TL;DR: The structure of carp muscle calcium-binding parvalbumin has been refined to an overall residual of 0.25 by a combination of model building and difference Fourier analyses, and these procedures and results are applicable to other protein refinement problems.

TL;DR: It is proposed that the seven α and seven β bands are alternative sets of sites which bind equivalently to complementary groups of sites on seven actins in the “relaxed” and “active” states of muscle, respectively.

TL;DR: A non-integrated form of Epstein-Barr virus DNA was purified from the Burkitt lymphoma-derived human lymphoid cell line Raji by CsCl density gradient centrifugation and neutral glycerol gradient centrifugalation, confirming the covalently closed circular duplex structure of part of the intracellular viral DNA.

TL;DR: Turbidometric measurements of the kinetics of filament assembly revealed a biphasic mechanism, involving the initial rate-limiting formation of a hexamer nuclear particle followed by a more rapid rate of polymerization to filaments.

TL;DR: The crystal structure of sodium guanylyl-3′,5′-cytidine (GpC) nonahydrate has been determined by X-ray diffraction procedures and refined to an R value of 0.054 and exhibits face-sharing octahedral co-ordination.

TL;DR: An hypothetical structural complex of cytochromes c and b5 which optimizes intermolecular complementary charge and steric interactions was generated by a least-squares fitting process which constrain the two heme prosthetic groups to be nearly coplanar at a closest approach distance.

TL;DR: The detailed results show that the displacement of the iron atom from the plane of the porphyrin ring increases from 0·40 to 0·55 A on going from met to deoxy.

TL;DR: The crystal structure of sodium adenylyl-3′,5′-uridine (ApU) hexahydrate has been determined by X-ray diffraction procedures and refined to an R factor of 0.057.

TL;DR: The method has been shown to be successful in determining the similarity of the NAD binding domains of lactate dehydrogenase and glyceraldehyde-3-phosphate dehydrogensase, and in comparing the heme binding fold of cytochrome b 5 with the globins.

TL;DR: The results suggest that the events of the cell cycle occur in a proscribed order because many of the gene products that mediate these events arc restricted to a prescribed sequence of function.

TL;DR: The crystal structure of yeast tRNA Phe is refined at 2·5A resolution, using real-space refinement and improved electron density maps to confirm almost all the structural details found earlier by fitting a wire model to the map in an optical comparator.

TL;DR: Unique, three-dimensional structures have been determined for Escherichia coli small subunits, large Subunits and monomeric ribosomes by electron microscopy of Ribosomes and subunits and of antibody-labeled ribosome and sub units.

TL;DR: The conformational role of a side chain is shown to be dependent on hydrophobicity and its ability to receive or donate a hydrogen bond and the application of the parameters to making predictions is demonstrated by a worked example for hen egg whito lysozyme.

TL;DR: It is concluded that the transforming gene(s) of all avian sarcoma viruses have closely related or common genetic lineages distinct from the transforming genes in sarcomA viruses of other species.

TL;DR: The main finding of this work is the recognition of phylogenetic differences at the macromolecular level in the organization of eukaryotic genomes.

TL;DR: Results presented here suggest that the acid form is produced as an intermediate in the first stage of total unfolding at neutral pH, which may cause a difference in dynamic character in the native state between the two proteins and thus provide a reasonable interpretation for their known differences in chemical reactivity.

TL;DR: The study of kinetics of polyphenylalanine synthesis and dependence of the synthesis rate on the Mg 2+ concentration in the factor-free, EF-T u -dependent and EF-G-dependent translation systems has demonstrated that the elongation factors with GTP promote ribosomal mechanisms of aminoacyl-tRNA binding and translocation, respectively.

TL;DR: Regulatory light chains which bind to desensitized scallop myofibrils with high affinity and restore calcium control were found in a number of molluscan and vertebrate myosins, including Mercenaria, Spisula, squid, lobster tail, beef heart, chicken gizzard, frog and rabbit.

TL;DR: Production of protein X is part of a feedback loop that limits DNA degradation and allows DNA repair, and is suggested to be an indirect consequence of DNA synthesis inhibition.

TL;DR: DNAs from three mammals, two amphibians, two invertebrates and a unicellular eukaryote, enzymatically degraded to molecular weights in the 1×106 to 2×106 range, were analyzed in their sedimentation coefficients, and modal and mean buoyant density of their CsCl bands.