Showing papers in "Journal of Structural Biology in 2003"

TL;DR: Two computer programs are presented, CTFFIND3 and CTFTILT, which determine defocus parameters from images of untilted specimens, as well as defocus and tilt parameters from image of tilted specimens, respectively, using a simple algorithm.

TL;DR: This work shows how its unique triple-helix structure and extremely high level of crystallinity make it exceptionally efficient in generating the second harmonic of incident light leads to a novel mode of microscopy of immediate practical significance in medicine and biology.

TL;DR: The cubic-phase method is described from the following perspectives: how it is done in practice, its general applicability and successes to date, and the nature of the mesophases integral to the process.

TL;DR: The enzymatic decellularization process of heart valves revealed a time-dependent loss of cells, ECM components and biomechanical strength, suggesting ECM-integrity may be compromised with prolonged incubation.

TL;DR: Results and guidelines for the initial screening of crystallization conditions, applicable to both manual and robotic setups, are discussed and a facility that executes the rapid survey of crystallized lead conditions is described in detail.

TL;DR: X-ray diffraction, energy-dispersive X-ray analysis, and infrared spectroscopy indicate that the mineral phase in the fish scale is calcium-deficient hydroxyapatite containing a small amount of sodium and magnesium ions, as well as carbonate anions in phosphate sites of the apatite lattice.

TL;DR: Applications and recommendations for seeding are presented based on examples from the laboratory and other groups and the implementation of seeding in high-throughput crystallization, robotics, and other emerging technologies is discussed.

TL;DR: Measured dimensions and polymer statistical analyses were used in conjunction with the results of Western analyses, chromatographic, and carbohydrate electrophoresis measurements to better understand the dependence of aggrecan structure and properties on its constituent GAG chains.

TL;DR: The polymerisation of monolignols is affected by the arrangement of the polysaccharides which constitute the cell wall, and it is concluded that lignin closely follows the cellulose microfibril orientation in the secondary cell wall.

TL;DR: The possibility of altering the intrinsic characteristics of a protein for crystallization has become a feasible strategy and historical perspectives and advances in this area will be reviewed.

TL;DR: The crystal structure of murine p97 in which D2 domain has been modelled as poly-alanine and the remaining approximately 100 residues are absent illustrates a head-to-tail packing arrangement of the two p97 AAA domains in a natural hexameric state with D1 ADP bound and D2 nucleotide free.

TL;DR: The results are surprising in that they show that classification can be done with less than one photon per pixel in the limiting resolution shell, assuming Poisson-type photon noise in the image.

TL;DR: A software tool based on anisotropic nonlinear diffusion is described for filtering cryotomograms, which reduces local noise and meanwhile enhances both curvilinear and planar structures.

TL;DR: The molecular deposition of starch extracted from normal plants and transgenically modified potato lines was investigated using a combination of light microscopy, environmental scanning electron microscope (ESEM) and confocal laser scanning microscopy (CLSM).

TL;DR: High-throughput crystallography offers the hope of correcting the imbalance in membrane protein structure, with significant technical challenges being facile protein overexpression and reliable methods for crystal growth.

TL;DR: The tight bonding of mineralized cartilage to bone suggests a mechanical role for the interface of the two elastically different tissues, bone and cartilage.

TL;DR: In this paper, it is shown that the classical approach explains the available nucleation data under most conditions used for growing protein crystals for structural studies and for industrial crystallization, but it fails to explain most experimental data on the structure of the critical clusters.

TL;DR: The implementation of this technique linked to the advancement of current crystallography software leads to a powerful structure determination method consolidating crystal growth, X-ray data collection, and ab initio phase determination into one without crystal manipulation.

TL;DR: It is argued that variability of mixing in batch crystallization may be a source of the variation in the number of crystals ultimately appearing in the sample.

TL;DR: This work introduces a topology representing network (TRN) method, which is clearly superior to both the multi-variate statistical analysis (MSA) and the self-organizing map (SOM) as a classification method and provides a first reliable classification method which can be used without masking for very noisy images.

TL;DR: Static and dynamic light scattering are discussed as particularly useful tools for studying various aspects of protein crystal growth and protein-protein interactions determined by light scattering serve as a predictor for favorable crystallization conditions as well as for protein solubility behavior.

TL;DR: This paper investigates the effectiveness of a bilateral denoising filter in various biological electron microscopy applications and finds that bilateral filter holds a distinct advantage in being capable of effectively suppressing noise without blurring the high resolution details.

TL;DR: A multidisciplinary approach that must invoke techniques from molecular biology, protein biochemistry, biophysics, artificial intelligence, and automation is needed to increase the success rate of high-throughput protein crystallization.

TL;DR: The three-dimensional structure of a single cell in conjunction with two extracellular cannulae, as determined by cryo-electron microscopy, indicates that the Cannulae interconnect individual cells with each other on the level of their periplasmic space; the authors do not, however, have evidence that they enter the cytoplasm of the cells.

TL;DR: High-spatial-resolution XANES maps for the different S species across adjacent prisms show that sulfate is the principal component in both the intraprismatic organic matrices and the outer membranes and confirm that the inner structures of the prismatic units are different for both genera.

TL;DR: Analysis of the annular substructure of demosponge biosilica spicules reveals that the deposited material is nanoparticulate, with a mean particle diameter of 74+/-13 nm, and the heirarchical organization of these nanoparticles is biologically unique.

TL;DR: This work focuses on a regulatory DNA-binding protein in the Gram-negative soil bacterium Sinorhizobium meliloti 2011, which is capable of fixing molecular nitrogen in a symbiotic interaction with alfalfa plants.

TL;DR: The alternate osteon structure is described by a rather continuous multidirectional pattern, in which dark and bright lamellae display different mechanical and possibly biological functions.

TL;DR: How the more-complex structures can be understood by extending Crick's principles for classic coiled coils, including coiled-coil oligomer state, is described.

TL;DR: Results indicated that stress proteins are involved in the process of microspore embryogenesis induction, and their association at specific stages have been determined between the two systems coexisting in the same culture.