Journal•ISSN: 1046-2023
Methods
Elsevier BV
About: Methods is an academic journal published by Elsevier BV. The journal publishes majorly in the area(s): RNA & Medicine. It has an ISSN identifier of 1046-2023. Over the lifetime, 5011 publications have been published receiving 373804 citations.
Topics: RNA, Medicine, Computer science, DNA, Chromatin
Papers published on a yearly basis
Papers
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TL;DR: The 2-Delta Delta C(T) method as mentioned in this paper was proposed to analyze the relative changes in gene expression from real-time quantitative PCR experiments, and it has been shown to be useful in the analysis of realtime, quantitative PCR data.
139,407 citations
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TL;DR: TrackMate is an extensible platform where developers can easily write their own detection, particle linking, visualization or analysis algorithms within the TrackMate environment and is validated for quantitative lifetime analysis of clathrin-mediated endocytosis in plant cells.
2,356 citations
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TL;DR: The print-tip loess normalization as mentioned in this paper is a well-tested general purpose normalization method which has given good results on a wide range of arrays and can be refined by using quality weights for individual spots.
2,084 citations
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TL;DR: Benefits of real-time PCR include enhanced sensitivity, high throughput, use of a closed-tube system, reduced variation, the ability to simultaneously multiplex reactions, and lack of post-PCR manipulations.
2,021 citations
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TL;DR: A collection of protocols to culture MCF-10A cells, to establish stable pools expressing a gene of interest via retroviral infection, as well as to grow and analyzeMCF- 10A cells in three-dimensional basement membrane culture are provided.
1,957 citations