Showing papers in "Micron in 1993"

TL;DR: This review summarizes present knowledge about the molecular structure of ferritin and apoferritin, with emphasis on the research results obtained by electron microscopy, and presents some remaining research questions where electron microscope studies are likely to provide valuable answers.

TL;DR: This review addresses the problem of understanding nucleolar morphology in terms of nucleolar function by reviewing in situ-cytochemical results that have not led to a generally accepted view on the structure-function correlation for nucleoli.

TL;DR: This review provides a summary of the conclusions that have been drawn from studies, and attempts to relate these observations to models for the role of RecA protein in homologous recombination.

TL;DR: There is sufficient evidence to indicate that genetic manipulation of cytoskeletal components is one path toward future direct manipulation of cell expansion characteristic in many plant systems and may lead to improvements in the textile qualities of cotton fibers.

TL;DR: It is shown that the use of SNARF-1, in conjunction with confocal microscopy is a powerful technique for accurately mapping pH within single cells, multicellular tissues and intact organs, as well as for accurately recording dynamic changes in pH.

TL;DR: Electron microscopy and other nonultrastructural morphological techniques have proven valuable tools in studying the pathology of EAU due to the highly organised and heterogeneous nature of the affected cells and tissues within the eye, namely the neural retina, retinak pigment epithelium and choroid.

TL;DR: A variety of data now suggest that TJ are highly dynamic structures, sensitive to envronmental and cellular factors, and able to control the paracellular pathway depending on physiological requirements, presumably through regulatory mechanisms and interactions with the cytoskeletal microfilament system.

TL;DR: Cytochemical and immunocytochemical approaches have shown that elastin fibres contain proteoglycans as well as sites recognized by antibodies to lysyl oxidase and microfibrillar components, and it has been suggested thatmicrofibrils and proteoglyCans might have a role in elastIn fibrogenesis.

TL;DR: Measurements on isolated retinal and pineal photoreceptors of the trout Salmo irideus reveal two different kinds of cells showing two distinct types of pigments, the former having its λmax at 463 nm and the latter with its maximum at 561 nm.

TL;DR: Using the pH-sensitive dual-emission fluorophore, carboxy-seminaphthorhodaflour-1 (SNARF-1) coupled with a laser-scanning confocal microscope system it is now possible to map intracellular pH, indicating that active control of intranuclear pH by the nuclear membrane occured, possibly via the K + /H + exchangers.

TL;DR: It has been found that BT, besides being well preserved when fixed in the presence of tannic acid, are sensitive to factors known to depolymerize cytoplasmic microtubules in a variety of cells such as nocodazole and cold and show a positive immunoreaction against different antitubulin antibodies.

TL;DR: In this paper, the degradation of starch-plastic films in soil was monitored using scanning electron microscopy, where the films were buried in a forest soil where rapid colonization by fungal hyphae could be detected.

TL;DR: Possible factors affecting both axonal NF content and interfilament distance are discussed; these include the ratio of NF subunits, the level of phosphorylation of these subunit, interactions with target cells, and interactions with ensheathing glial cells.

TL;DR: The applications of various cryofixation and cryosubstitution methods in the study of the cell nucleus are summarized and an attempt to critically evaluate their results is made in this review.

TL;DR: In this article, a microscopic method for the determination of bacterial biovolume using epifluorescence microscopy is presented, which combines direct observations of the microscopic picture with a data processing unit.

TL;DR: Intensity-modulated Multiple-beam Scanning (IMS) microfluorometry has the potential to virtually eliminate the cross-talk between fluorophores that often occurs when recording multiple-labelled specimens.

TL;DR: In this paper, the degradation of poly-β-hydroxyalkanoate (PHA) films was monitored using both light and scanning electron microscopy (SEM) for up to three months.

TL;DR: In this paper, the structure of mouse skin collagen fibrils treated with 500 rad gamma irradiation was studied by electron microscopy, and after 1, 4 and 8 weeks of treatment, the results revealed that the fibril sizes were consistently larger in diameter than those from normal specimens with the majority of irradiated fibrILS measuring 110-138 nm, as opposed to 62-84 nm in the control.

TL;DR: In this paper, the authors developed a newly designed ultraviolet laser-scanning confocal microscopy (UV-LSCM) system and applied it for quantitative confocal imaging of intracellular calcium concentration ([Ca2+]i) with the dual-emission wavelength indicator indo-1.

TL;DR: It is shown that whole mount preparations of embryonic chick sterna can be analyzed with confocal laser scanning microscopy (CLSM), and single label in situ hybridization studies show that chondrocytes are positive for type II collagen mRNA.

TL;DR: In this article, the authors summarized ultrastructural and conformational information on LPS, with special attention to electron microscopy and crystallization of LPS and found that R-form LPSs, lacking the O-specific polysaccharide portion, crystallize by treatment with ethanol containing MgCl2.

TL;DR: In this paper, the design, construction and performance of two single fast-freezing devices are described, driven pneumatically at a controlled speed of up to 100msec -1.

TL;DR: NMR microscopy of mature gooseberry fruits using the gradient-echo method clearly delineated the ovary wall from the parenchyma tissues which surround seeds in the locular cavity, and the entire vascular architecture of the fruit was revealed.

TL;DR: In this article, the experimental results on fibre-optical confocal scanning microscopy (FOCSM) are reported, and it is shown convincingly that the FOCSM is a purely coherent imaging system as predicted in our previous theory.

TL;DR: Ulastructural analysis of the absorbent cells in the distal intestine of the rainbow trout Oncorhynchus mykiss revealed the presence in this zone of two types of cells: vacuolated and non-vacuolated, which suggests that these cells are involved in the uptake of dietary protein macromolecules by pinocytosis.

TL;DR: This review compares the available methodologies and presents some applications of light and ultrastructural immunogold labelling, which are effective in indicating the presence, distribution and relative abundance of antigens in cellular systems.

TL;DR: The increasing availability of both polyclonal and monoclonal antibodies has made possible the EM immunolabelling of dystrophin; immunogold on cryosections being the technique of choice.

TL;DR: In this paper, changes in the relative intensity of ionization edges with increasing specimen thickness are shown to arise from the influence of elastic scattering on the energy-loss spectrum, and the resulting error in measured elemental composition can be minimised by using a medium-sized (e.g. 20 mrad) collection aperture, or may be corrected for by applying the Lenz model of elastic scat.

TL;DR: In this paper, the addition of a macro-molecular solute (PVP) facilitated production of thin droplets and reduced crystal size by a very simple method involving slow drying under liquid paraffin oil.