Molecular marine biology and biotechnology 

About: Molecular marine biology and biotechnology is an academic journal. The journal publishes majorly in the area(s): Gene & Complementary DNA. It has an ISSN identifier of 1053-6426. Over the lifetime, 249 publications have been published receiving 21456 citations.

DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates.

Abstract: We describe "universal" DNA primers for polymerase chain reaction (PCR) amplification of a 710-bp fragment of the mitochondrial cytochrome c oxidase subunit I gene (COI) from 11 invertebrate phyla: Echinodermata, Mollusca, Annelida, Pogonophora, Arthropoda, Nemertinea, Echiura, Sipuncula, Platyhelminthes, Tardigrada, and Coelenterata, as well as the putative phylum Vestimentifera. Preliminary comparisons revealed that these COI primers generate informative sequences for phylogenetic analyses at the species and higher taxonomic levels.

Genetic immunization of rainbow trout (Oncorhynchus mykiss) against infectious hematopoietic necrosis virus.

Abstract: Plasmid vectors encoding the infectious hematopoietic necrosis virus (IHNV) nucleoprotein or glycoprotein gene under the control of a cytomegalovirus promoter were used to immunize rainbow trout (Oncorhynchus mykiss) against IHNV. The plasmid DNA was injected into the skeletal muscle of rainbow trout fry, and immunization was determined by the detection of virus-neutralizing and enzyme-linked immunosorbent assay antibody activity, and by protection against live virus challenge. Fish injected with the glycoprotein-encoding plasmid pCMV4-G, either alone or in combination with the nucleoprotein-encoding plasmid pCMV4-N, generated glycoprotein-specific and virus-neutralizing antibody responses. The vaccinated fish were also protected from subsequent IHNV challenge. Fish receiving pCMV4-N alone did not produce measurable virus-specific antibody and were killed by IHNV infection. These studies show that DNA vaccination will protect rainbow trout against the lethal effects of IHNV infection.

Large mitochondrial DNA differences between morphologically similar Penaeid shrimp.

Abstract: Sequencing of mtDNA fragments amplified with the polymerase chain reaction is used to examine genetic differences between species of Penaeid shrimp. The data show surprisingly high genetic differences among morphologically and ecologically similar species. Differences at silent sites between subgenera of shrimp are larger than between families of mammals. Between genera, Penaeid shrimp are more divergent than some orders of mammals. Because taxonomic differences are defined on morphological grounds, these results imply that the rates of molecular versus morphological evolution in shrimp are different than in mammals. Perhaps mtDNA evolution is accelerated in shrimp, or perhaps stabilizing selection over a long time period has reduced rates of morphological change. For Penaeid shrimp, morphological similarity can mask large genetic differences.

Field preservation of marine invertebrate tissue for DNA analyses.

Abstract: Successful preservation of tissue samples is a prerequisite for long field studies in remote areas However, there is little published information concerning field preservation of marine invertebrate tissues for DNA analyses This omission is significant because marine biodiversity is centered in the Indo-Pacific, where immediate DNA analysis is often impossible Consequently, we used an assay based on polymerase chain reaction (PCR) to examine the effect of five storage solutions and three temperature regimens on the degradation of DNA from four common classes of marine invertebrates (Anthozoa, Gastropoda, Polychaeta, and Scyphozoa) Control samples were cryopreserved Storage solution and the type of tissue preserved were the best predictors of preservation success The length of time in storage and the storage temperature also affected the preservation of DNA A field test demonstrates that a solution of dimethylsulfoxide and sodium chloride (DMSO-NaCl) preserves a wide range of tissues for DNA analyses and is very simple to use in remote field locations

Universal cytochrome b primers facilitate intraspecific studies in molluscan taxa.

Abstract: We describe the construction of amplification primers designed to target a portion of the mitochondrial cytochrome b locus in a variety of molluscan taxa. Combinations of two sets of primers successfully amplified cytochrome b from several species of gastropods, bivalves, and cephalopods. Sequence analysis of these amplified products revealed nucleotide diversity in small samples within several of these taxa. We discuss the utility of these primer sets for studies of intraspecific phylogeny in mollusks and potentially other invertebrates.