National Medical Journal of China 

About: National Medical Journal of China is an academic journal published by Chinese Medical Association. The journal publishes majorly in the area(s): Medicine & Transplantation. It has an ISSN identifier of 0376-2491. Over the lifetime, 11300 publications have been published receiving 45482 citations. The journal is also known as: National Medical Journal of China.

[Expression of fusion proteins in beta(2)GP I gene-transfected HEp-2 cells and its clinical application].

Abstract: OBJECTIVE To establish an indirect immunofluorescent test so as to improve the sensitivity and specificity of examination of antibodies to beta(2)-glycoprotein METHODS Full-length beta(2)GP cDNA was obtained from human hepatocellular cancer cell line HepG2 by RT-PCR and cloned into the mammalian expression vector pEGFP-C1 The recombinant plasmid pEGFP-beta(2)GP was transfected into HEp-2 cells RT-PCR, immunoblotting (IBT), confocal fluorescence microscopy, and indirect immunofluorescent test (IIF) were used to confirm the expression, localization, and antigenicity of fusion protein of green fluorescent protein (GFP) Serum specimens from 19 patients suspected as with secondary antiphospholipid syndrome (APS), 1 patient diagnosed as with primary APS, and 10 normal persons were detected with IIF-IgG-beta(2)GP1, ELISA-IgG-ACL, and ELISA-IgG-beta(2)GP I simultaneously RESULTS (1) The HEp-beta(2)GP I cells thus obtained retained their ability of expression of beta(2)GP-I-GFP for more than ten generations This beta(2)GP-I-GFP showed the antigenicity of beta(2)GP-I with a characteristic feature (2) Seven of the 20 serum specimens from APS patients showed characteristic immunofluorescent pattern No serum specimen from normal persons showed immunofluorescent staining The comparison of results of the three methods showed that the concordance between IIF-IgG-beta(2)GP I and ELISA-IgG-beta(2)GP I was the most perfect (Kappa = 0886) (3) HEp-beta(2)GP I retained the immunofluorescent property of HEp-2 cell CONCLUSION As a new kind of substrate of IIF, beta(2)GP I transfectant can be used to detect anti-beta(2)GP-I antibodies Transfeted HEp-2 cells keep the immunofluorescent property of HEp-2 cells in IFANA test and can be used as substrate for routine IFANA detection

[Association of 5-HT(2A) receptor polymorphism and attention deficit hyperactivity disorder in children].

Abstract: OBJECTIVE To stude the association of 5-HT(2A) receptor polymorphism and attention deficit hyperactivity disorder (ADHD) in children. METHODS Blood samples were taken from 323 6 approximately 17.5-year-old children with ADHD disgnosed based on the DSM-IV criteria, 182 healthy 18 approximately 49-year-old controls, and 195 pairs of parents of affected children, all of Han nationality. DNA was extracted. PCR was performed to examine the 5-hydroxytryptamine (5-HT)(2A) receptor T102C polymorphism. Transmission disequilibrium test (TDT) was used to test the association of alleles of 5-HT(2A) T102C polymorphic sites and AFDHD. RESULTS The frequency of T102T in patients with ADHD combined subtype was lower than that in the controls (22.35 vs 33.5%, OR = 0.569, P = 0.028, 95% CI 0.344 - 0.943), and the frequency of T102C in patients with ADHD combined subtype was higher than that in the controls (64.0% vs 47.3%, OR = 1.987, P = 0.003, 95% CI 1.264 approximately 3.124). TDT showed biased transmission of the alleles of T102C polymorphism among families of girl patients with ADHD combined subtype (P = 0.031). CONCLUSION For the ADHD combined subtype, the T102T genotype is a protective factor and the T102C genotype is a risk factor. For the girl with ADHD combined subtype, the allele C102 is a disease-predisposing gene.

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Abstract: Poly (ionic liquid)s attracted enormous attention as adsorbents for the CO2 separation from natural gas. Hence, in this work poly (1-butyl-3-vinylimidazolium bromide), P[VBIm][Br], poly (1-butyl-3-vinylimidazolium thiocyanate), P[VBIm][SCN], and poly (1-butyl-3-vinylimidazolium tetrafluoroborate), P[VBIm][BF4] were synthesized and evaluated their CO2 adsorption performance. The synthesized poly(ionic liquid)s were characterized by 1H NMR, FT-IR spectroscopy, and differential scanning calorimetery (DSC) analysis. The CO2 adsorption was studied at various temperatures and pressures by quartz crystal microbalance (QCM) and experimental data were correlated by a model of dual-mode. The Henry and Langmuir contributions in CO2 adsorption were evaluated. The obtained thermodynamics and kinetics parameters of CO2 adsorption reveal that CO2 adsorption has an exothermic and physisorption nature. Also, density functional theory (DFT) computations were done in order to assess interactions between poly(ionic liquid)s with CO2 gas. DFT computations corroborated that interaction of P[VBIm][SCN] with CO2 is stronger than those of other poly(ionic liquid)s.