Showing papers in "Nitric Oxide in 2001"

TL;DR: A method for simultaneous evaluation of nitrate and nitrite concentrations in a microtiter plate format is developed and S-Nitrosothiols and L-arginine derivatives were found to be potential interfering agents.

TL;DR: One target for new drugs should be the preservation or restoration of NO-mediated signaling pathways in arteries, which may include administration of l-arginine/antioxidants and gene-transfer approaches.

TL;DR: It is shown that the growth of three common urinary pathogens is markedly inhibited in mildly acidified urine when nitrite is present, which may help to explain the well-known beneficial effects of urinary acidification with, e.g., vitamin C in treatment and prevention of urinary tract infection.

TL;DR: Support is provided for the hypothesis that a hemodynamic change results in increased shear stress in the liver causing generation of NO, which then triggers the liver regeneration cascade.

TL;DR: Results provide substantial evidence to confirm that the plant alternative pathway is resistant to NO and support the idea that the alternative pathway may lower respiration-dependent production of active oxygens under conditions where NO is overproduced.

TL;DR: The results from this study strongly support the proposal for a separate pool of arginine for nitric oxide production that is defined by the cellular colocalization of enzymes involved in nitricoxide production and the regeneration of arkinine.

TL;DR: Based on a limited number of patients, this work suggests that individuals who are NOS3-CC + ACE-DD are at a higher risk for early CAD, probably as a consequence of increased endothelial dysfunction.

TL;DR: It is shown that either the RBC membrane permeability to NO or the intracellular reaction rate between NO and hemoglobin (Hb) is at least 2000-fold lower than previously thought and may play an important role in NO bioavailability.

TL;DR: It is demonstrated that NAC administration can modulate the massive NO production induced by LPS, and can be attributed mostly to the inhibitory effect of NAC on one of the events leading to iNOS protein expression.

TL;DR: The results suggest the presence of multiple pathways to APAP-mediated hepatic necrosis, one via nitrotyrosine, as in the wild-type mice, and another that is not dependent upon inducible nitric oxide synthase activity, but which may involve increased superoxide.

TL;DR: To address confusion that has arisen in the literature resulting from rather widespread use of differing trivial designations for this class of compounds, a unifying nomenclature system is recommended in which compounds containing the [N(O)NO]- moiety are named as diazeniumdiolates.

TL;DR: H( 2)O(2) production from LPS-stimulated macrophages participates in the upregulation of inducible nitric oxide synthase (iNOS) gene expression via NF-kappa B activation and that NO is a negative feedback inhibitor of iNOS protein expression are suggested.

TL;DR: It is argued that production of ONO2(-) is an important contributor to radiation damage in biological systems and the implications of these findings in relation to possible mechanisms of oxidant-linked apoptosis are briefly considered.

TL;DR: It is shown that, in addition to its antioxidative property, GSPE enhances low-level production of intracellular NO in primary rat astroglial cultures and protects the microglial GSH pool during high output NO production and results in an elevation of the H( 2)O(2) tolerance in astrogLial cells.

TL;DR: This model is associated with typical features of human septic shock, including low systemic vascular resistance, altered myocardial function and limited oxygen extraction capability and may contribute to a better delineation of the involvement of NO in endotoxic shock, and the evaluation of the therapeutic effects of NO inhibitors.

TL;DR: The phagocytic activity of Mytilus galloprovincialis hemocytes is thought to be associated with NADPH-oxidase activity of the plasma membrane, thus producing superoxide anions, and nitric oxide release by these haemocytes is investigated in order to understand its role in the defensive mechanisms of these organisms.

TL;DR: It appears that eNOS activation and subsequent NO release may act as a regulatory system to counter balance the potentially deleterious effects of myocardial ischemia/reperfusion.

TL;DR: It is concluded that the 2-electron redox cycling of the pterin cofactor between BH4 and quinonoid dihydrobiopterin is not essential for NO synthesis.

TL;DR: The results indicate that eNOS and AS are coinduced in the aorta in early stages of STZ-induced diabetic rats and that the induction is mediated by TGF-beta1, and suggest that TGF -beta1 works antiatherogenically atEarly stages of diabetes by increasing NO production, whereas prolonged elevation of T GF- beta1 functions atherogenically by inhibiting endothelial cell growth.

TL;DR: Results imply that there may be a novel nitric oxide synthase in muscle and that NO.

TL;DR: Results indicate that the JAK family of protein kinases and the transcription factor NF-kappa B are involved in the induction of iNOS protein expression in FSDC stimulated with LPS.

TL;DR: Results indicated l-arginine-dependent production of NO to be one of the effector mechanisms contributing to the tumoricidal activity of MCP-1-treated macrophages, and indicated the regulatory role of calcium/CaM in the process of M CP- 1-induced macrophage NO production.

TL;DR: Sponges are the most primitive metazoan group; their evolution dates back 600 million years; the presence of environmental stress-activated NOS activity in these organisms may prove to be the most ancient NO-dependent signaling network in the animal kingdom.

TL;DR: In both dissociated dorsal root ganglion (DRG) cultures and in acutely isolated, but intact, DRG, treatment with capsaicin or bradykinin caused cGMP synthesis, which could be blocked by NOS inhibitors, leading to the suggestion that NO acts in a paracrine fashion when stimulating the synthesis of cG MP and may not be synthesized at all sites containing nNOS.

TL;DR: The results obtained indicate that augmentation of free radical generation from rat PMNs following LPS treatment appears to be regulated by NO and MPO.

TL;DR: It is concluded that acute administration of SNAP in the oral glucose tolerance test releases NO that modulates the parameters of carbohydrate metabolism and results in sustained hyperglycemic effect observed in SNAP-treated dogs.

TL;DR: From a biological point of view, it is important to point out that GSH reacts with NO in the presence of ferrimyoglobin, even at physiological pH, to form nitrosoglutathione.

TL;DR: Both types of enzymes mediating nitric oxide affect inflammatory responses, i.e., vascular hyperpermeability, and their regulation may lead to the development of new therapy for thermal injuries.

TL;DR: There are at least three mechanisms of synergistic cytokine-induced NO production: the biosynthesis of iNOS monomer due to nonlinear interactions by transcription factors, synergistic cytosolic activation of NF-kappaB, and parallel biosynthesisof BH4 in the presence of cytokine combinations that include IL-1beta.

TL;DR: A fluorescence assay for nitric oxide synthase activity in skeletal muscle based on a new indicator, 4,5-diaminofluorescein (DAF-2) allows real-time measurement of NO production and is safer and more convenient than the usual citrulline radioassay.