Showing papers in "Plant and Cell Physiology in 1990"
TL;DR: An Intron-GUS reporter gene which contains a modified intron of the castor bean catalase gene within the N-terminal part of the β-glucuronidase coding sequence was constructed, suggesting splicing of the intron occured in tobacco cells.
Abstract: An Intron-GUS reporter gene which contains a modified intron of the castor bean catalase gene within the N-terminal part of the β-glucuronidase (GUS) coding sequence was constructed. When the Intron-GUS reporter gene placed downstream of the cauliflower mosaic virus (CaMV) 35S promoter (35S-Intron-GUS) was introduced into protoplasts of tobacco suspension-cultured cells by electroporation, expression of GUS activity was observed suggesting splicing of the intron occured in tobacco cells
421 citations
TL;DR: The chemical composition of phloem sap from the uppermost internode of rice plants, one week after anthesis, was compared with that ofphloem Sap from the leaf sheath of a young seedling.
Abstract: The chemical composition of phloem sap from the uppermost internode of rice plants, one week after anthesis, was compared with that of phloem sap from the leaf sheath of a young seedling. The pure phloem sap from rice plants was collected by an insect laser technique
218 citations
TL;DR: In this report, it is provided evidence, that in the endosperm of Ricinus also the H 2 O 2 scavenging pathway involving ascorbate and glutathione plays a significant role in H2 O 2 degradation.
Abstract: In this report we provide evidence, that in the endosperm of Ricinus also the H 2 O 2 scavenging pathway involving ascorbate and glutathione plays a significant role in H 2 O 2 degradation
210 citations
TL;DR: The cloned cDNA contained an open reading frame of 1479 base pairs encoding a sequence of 493 amino acids and a putative pyridoxal phosphate binding site of the enzyme is suggested.
Abstract: (...) The cloned cDNA contained an open reading frame of 1479 base pairs encoding a sequence of 493 amino acids. Identification of the cDNA was accomplished by expression of active enzyme in Escherichia coli harboring the cDNA and by the presence of a partial amino acid sequence identical to that found in the purified enzyme. A putative pyridoxal phosphate binding site of the enzyme is suggested. (...)
159 citations
103 citations
TL;DR: The present investigation was undertaken in order to examine the influence on nitrogen fixation of the accumulation of nitrite, as result of the supply of exogenous nitrate, in soybean nodules, and to clarify the relationship between the inhibition of nitrogen fixation, the formation of Nitrite in the nodule cytosol, and the decrease in leghemoglobin function due to the Formation of LbNO under such conditions.
Abstract: The present investigation was undertaken in order to examine the influence on nitrogen fixation of the accumulation of nitrite, as result of the supply of exogenous nitrate, in soybean nodules, and to clarify the relationship between the inhibition of nitrogen fixation, the formation of nitrite in the nodule cytosol, and the decrease in leghemoglobin function due to the formation of LbNO under such conditions
100 citations
TL;DR: The accumulation of betaine and the induction of BADH activity in barley leaves during salt stress is investigated and levels of badh activity in etiolated leaves and roots of barley are studied.
Abstract: In this study, we investigated the accumulation of betaine and the induction of BADH activity in barley leaves during salt stress. We also studied the accumulation of betaine and levels of BADH activity in etiolated leaves and roots of barley
89 citations
TL;DR: The effect of Bx-112 on shoot elongation caused by exogenously applied GAs was investigated in rice seedlings and the inhibitory sites of GA biosynthesis by BX-112 were discussed.
Abstract: The effect of BX-112 on shoot elongation caused by exogenously applied GAs was investigated in rice seedlings. The inhibitory sites of GA biosynthesis by BX-112 are also discussed
86 citations
TL;DR: Pure phloem sap was collected from leaf sheaths of Zea mays L. by the insect laser technique, and its chemical composition was analyzed, and Sucrose was the only sugar detected.
Abstract: Pure phloem sap was collected from leaf sheaths of Zea mays L. by the insect laser technique, and its chemical composition was analyzed. Sucrose was the only sugar detected. The predominant inorganic ions were K + and Cl − . The adenylate energy charge of phloem sap was between 0.72 and 0.86
74 citations
TL;DR: Un systeme acellulaire obtenu a partir de graines immatures de potiron, de haricot et de pois, est utilise pour l'etude d'un nouveau regulateur de croissance, l'acide 3,5-dioxo-4-propionylcyclohexanecarboxylique, sur la synthese des gibberellines.
Abstract: Un systeme acellulaire obtenu a partir de graines immatures de potiron, de haricot et de pois, est utilise pour l'etude d'un nouveau regulateur de croissance, l'acide 3,5-dioxo-4-propionylcyclohexanecarboxylique, sur la synthese des gibberellines. Les sites d'inhibition dans la chaine de biosynthese sont recherches
70 citations
TL;DR: The results suggest that the genotypes I and II are two highly divergent evolutionary lines that define a marked division of various phenotypes, such as production of rhizobitoxine, EPS composition and hydrogen uptake.
Abstract: Genomic digests from 25 strains of Bradyrhizobium japonicum, for which the phenotypes have been determined with respect to production of rhizobitoxine, hydrogen uptake (Hup) and composition of extracellular polysaccharide (EPS), were hybridized with probe DNAs of the nifDK and nifE genes of B japonicum USDA 110 The degree of the estimated base substitution in and around nifDKE clearly divided the strains of B japonicum into two markedly divergent groups, which were designated as genotype I and II Moreover, a strict correlation was observed between these genotypes, production of rhizobitoxine and EPS composition The genotype I strains produced no rhizobitoxine and an EPS composed of glucose, mannose, galactose, 4-Omethyl galactose and galacturonic acid, whereas the genotype II strains produced rhizobitoxine and an EPS composed of rhamnose and 4-O-methyl glucuronic acid Hup strains were confined exclusively to the genotype I Hind III digests of genomic DNAs from the 25 strains were hybridized with probe DNA of structural genes for the uptake hydrogenase from B japonicum In 23 wild-type strains, Hup strains generated a 59-kb band that hybridized to the probe under high-stringency conditions, while Hup" strains did not generate the band These results suggest that the genotypes I and II are two highly divergent evolutionary lines that define a marked division of various phenotypes, such as production of rhizobitoxine, EPS composition and hydrogen uptake
TL;DR: The investigation into the effets of external Ca 2+ on elongation and the intracellular concentration of K + in intact mung bean roots under saline conditions is described.
Abstract: In this report, we describe our investigation into the effets of external Ca 2+ on elongation and the intracellular concentration of K + in intact mung bean roots under saline conditions. We also discuss the mechanism of salt stress in mung bean roots and the role of the intracellular concentration of K + in root elongation under salt stress
TL;DR: The activity of RuBPcase in vivo was estimated by measuring the «initial» activity immediately after extraction from standard leaves and from sink-limited leaves.
Abstract: (...) In the present study, the activity of RuBPcase in vivo was estimated by measuring the «initial» activity immediately after extraction from standard leaves (grown under a regime of 10 h of light and 14 h of darkness) and from sink-limited leaves (exposed for 6 or 7 d to continuous light to alter the source/sink balance). (...)
TL;DR: The purpose of the present study was to investigate in greater detail the characteristics of photosynthesis in the rice panicle and to measure the enzymatic activities associated with the photosynthetic carbon metabolism in the Rice panicle in a comparison with those in the flag leaf.
Abstract: The purpose of the present study was to investigate in greater detail the characteristics of photosynthesis in the rice panicle and to measure the enzymatic activities associated with the photosynthetic carbon metabolism in the rice panicle in a comparison with those in the flag leaf
TL;DR: It is shown by measurements of chlorophyll fluorescence that in intact or broken, uncoupled chloroplasts the intersystem electron flow is severely blocked in the absence of oxygen, even when the Hill acceptor methyl viologen is present at millimolar concentration.
Abstract: In the present communication, we show by measurements of chlorophyll fluorescence that in intact or broken, uncoupled chloroplasts the intersystem electron flow is severely blocked in the absence of oxygen, even when the Hill acceptor methyl viologen is present at millimolar concentration
TL;DR: Gravity-induced changes in intracellular potentials in primary roots of 2-day-old mung bean seedlings indicates that they may result from stimulus-perception and transduction within the elongation zone rather than from transmission of a signal from the root cap.
Abstract: Gravity-induced changes in intracellular potentials in primary roots of 2-day-old mung bean (Vigna mungo L. cv. black matpe) seedlings were investigated using glass microelectrodes held by 3-dimensional hydraulic micro-drives. The electrodes were inserted into outer cortical cells within the elongation zone. Intracellular potentials, angle of root orientation with respect to gravity, and position within the root of the impaled cortical cell were measured simultaneously. Gravistimulation caused intracellular potential changes in cortical cells of the elongation zone. When the roots were oriented vertically, the intracellular potentials of the outer cortical cells (2 mm behind the root apex) were approximately - 115 mV. When the roots were placed horizontally cortical cells on the upper side hyperpolarized to - 154 mV within 30 s while cortical cells on the lower side depolarized to about - 62 mV. This electrical asymmetry did not occur in cells of the maturation zone. Because attempts to insert the electrode into cells of the root cap were unsuccessful, these cells were not measured. The hyperpolarization of cortical cells on the upper side was greatly reduced upon application of N,N'-dicyclohexylcarbodiimide (DCCD), an inhibitor of respiratory energy coupling. When stimulated roots were returned to the vertical, the degree of hyperpolarization of cortical cells on the previous upper side decreased within 30 s and approached that of cortical cells in non-stimulated roots. This cycle of hyperpolarization/loss of hyperpolarization was repeatable at least ten times by alternately turning the root from the vertical to the horizontal and back again. The very short (<30 s) lag period of these electrical changes indicates that they may result from stimulus-perception and transduction within the elongation zone rather than from transmission of a signal from the root cap.
TL;DR: Thirteen cDNA clones for the light-harvesting chlorophyll a/b binding protein of photosystem II (LHCPII) from rice were constructed; they were grouped into two classes that were based on restriction enzyme maps.
Abstract: Thirteen cDNA clones for the light-harvesting chlorophyll a/b binding protein of photosystem II (LHCPII) from rice were constructed; they were grouped into two classes that were based on restriction enzyme maps. The nucleotide sequences from two clones that belonged to different classes were determined and compared. The clones contained a 798-bp and 792-bp open reading frame capable of coding for polypeptides with 265 and 263 amino acids, respectively (...)
TL;DR: The polypeptides that accumulate in photoautotrophically cultured green tobacco cells, and in mesophyll tobacco cells as well as other cultured cells grown under different culture conditions are characterized to determine what mechanism controls gene expression in cultured cells.
Abstract: We characterized the polypeptides that accumulate in photoautotrophically cultured green tobacco cells, and in mesophyll tobacco cells as well as other cultured cells grown under different culture conditions to determine what mechanism controls gene expression in cultured cells
TL;DR: An assay system was developed in which the effects of inhibitors of β-carotene biosynthesis in Dunaliella bardawil could be tested, and it was found that D. bardawsil can be induced to accumulate over 10% of its dry weight as β- carotene.
Abstract: An assay system was developed in which the effects of inhibitors of β-carotene biosynthesis in Dunaliella bardawil could be tested. Since D. bardawil can be induced to accumulate over 10% of its dry weight as β-carotene, it is particularly suitable for such studies. (...)
TL;DR: The relationship between the accumulation of Chl and the apoproteins of the light-harvesting Chl a/b-protein complex of PS II (LHCII) during the greening of cucumber cotyledons was studied.
Abstract: The relationship between the accumulation of Chl and the apoproteins of the light-harvesting Chl a/b-protein complex of PS II (LHCII) during the greening of cucumber cotyledons was studied. LHCII apoproteins were not detected in etiolated cotyledons. Upon illumination, Chl a was formed as a result of photoconversion of protochlorophyllide (Pchlide) which had accumulated in the dark. (...)
TL;DR: A pea pathogen, Mycosphaerella pinodes, secretes both an elicitor and a suppressor for the accumulation of pisatin, a major phyotalexine of pea, into the Spore germination fluid.
Abstract: A pea pathogen, Mycosphaerella pinodes, secretes both an elicitor and a suppressor for the accumulation of pisatin, a major phyotalexine of pea, into the Spore germination fluid. The effects of the elicitor and the suppressor on the ATPase activity in pea plasma membranes was examined
TL;DR: Dans le cadre de l'elucidation du role physiologique des linoleate-oxygene oxydoreductase, la decomposition des hydroperoxydes de lipides est etudiee dans les semences de riz (Oryza sativa).
Abstract: Dans le cadre de l'elucidation du role physiologique des linoleate-oxygene oxydoreductase, la decomposition des hydroperoxydes de lipides, produits de l'activite de ces enzymes, est etudiee dans les semences de riz (Oryza sativa). L'activite responsable de cette decomposition est recherchee et les proprietes antifongiques des produits de la reaction sont analysees
TL;DR: The accumulation of putrescine under anoxia was studied in seedlings of 6 Gramineae species showing tolerance to the stress in the following order: rice, barnyard grass > maize > rye, barley and wheat.
Abstract: The accumulation of putrescine under anoxia was studied in seedlings of 6 Gramineae species showing tolerance to the stress in the following order: rice, barnyard grass > maize > rye, barley and wheat. The accumulation of putrescine in shoots and roots during 6 h of oxygen deprivation was different among the species and correlated with their tolerance to anaerobic conditions. In both tissues, rice and barnyard grass accumulated more than 0.6 μmol/g fresh weight. (...)
TL;DR: In this article, les variations de production of ribulosebisphosphate carboxylase and chlorophylle en reponse a la salinite sont mesurees dans les cellules de luzerne.
Abstract: Afin de relier l'expression des genes de la photosynthese avec le phenomene de tolerance a la salinite, les variations de production de ribulosebisphosphate carboxylase et de chlorophylle en reponse a la salinite sont mesurees dans les cellules de luzerne. L'importance d'un appareil photosynthetique fonctionnel dans la tolerance au sel est examinee
TL;DR: Dans le but d'etudier l'effet de la source d'azote sur le niveau des isoformes de la glutamine synthetase et de the glutamate synthase, des cultures cellulaires de riz (Oryza sativa) sont realisees.
Abstract: Dans le but d'etudier l'effet de la source d'azote sur le niveau des isoformes de la glutamine synthetase et de la glutamate synthase, des cultures cellulaires de riz (Oryza sativa) sont realisees. Les isoformes des deux enzymes sont analyses par immunoblotting et leurs activites sont mesurees
TL;DR: Using aDNA-binding assay, DNA-binding proteins were identified in the cp-nuclei from the mesophyll protoplasts of N. tabacum cv.
Abstract: In the present study, we have isolated cp-nuclei from the mesophyll protoplasts of N. tabacum cv. Bright Yellow-2, which is the mother plant of the line BY-2. Using a DNA-binding assay, DNA-binding proteins were identified in the cp-nuclei
TL;DR: Effects of cytG, d 1 d 2 , Gd 1 d 1 and Y 3 in a near isogenic background on photosynthetic rate plus some related parameters are studied to analyze the nature of these genetic changes and to dissect the process of monocarpic senescence.
Abstract: Effects of cytG, d 1 d 2 , Gd 1 d 2 and Y 3 in a near isogenic background on photosynthetic rate plus some related parameters and use of this information to analyze the nature of these genetic changes and to dissect the process of monocarpic senescence
TL;DR: In soybean (Glycine max [L.] Merr.) axis tissue and light- and dark-grown cotyledons 3 to 9 days of age, increasing amount of alternative oxidase corresponded with the increasing alternative pathway capacity of isolated mitochondria from these tissues, but from 9 to 12 days ofAge, little or no change in amount ofAlternative oxidase occurred in cotYledons even though there were substantial changes in capacity.
Abstract: In soybean (Glycine max [L.] Merr.) axis tissue and light- and dark-grown cotyledons 3 to 9 days of age, increasing amount of alternative oxidase corresponded with the increasing alternative pathway capacity of isolated mitochondria from these tissues. From 9 to 12 days of age, however, little or no change in amount of alternative oxidase occurred in cotyledons even though there were substantial changes in capacity. This suggests that, whereas alternative oxidase content may be important in determining the capacity of the alternative pathway in young cotyledons and in axis tissue, other factors regulate capacity in older cotyledons
TL;DR: This is the first report of the regeneration of plants from isolated microscope culture of indicas, considered to be recalcitrant cultivars.
Abstract: This is fhe first report of the regeneration of plants from isolated microscope culture of indicas, considered to be recalcitrant cultivars