Showing papers in "Protein Expression and Purification in 2017"

TL;DR: The ExpiCHO transient transfection systems allows for facile production of milligrams to grams of protein in CHO cells and de-risks the transition from transient to stable material during drug development.

TL;DR: A thorough characterisation of the genetics, physiology and metabolism of Escherichia coli has led to the availability of a large number of strains and vectors suitable for recombinant protein expression, and a number of factors can be considered and optimised for achieving required levels of amplified expression.

TL;DR: In this review, the research progress on heterologous expression of AMP using Escherichia coli, Bacillus subtilis, Pichia pastoris and Saccharomyces cerevisiae as host cells was mainly summarized, which might guide the expression strategies of AMPs in these cells.

TL;DR: The data showed that rPaDef derived from Mexican avocado fruit can be expressed and secreted efficiently when P. pastoris was used as a cell factory and holds great promise for antibacterial drug development.

TL;DR: It could be confirmed that the selected isolate is a potent strain and it can be able to produce xylanase through fermentation process and also it can able to convert the pretreated agro-wastes into economically important byproduct like bioethanol.

TL;DR: Effective HCP removing strategies at different stages of downstream process for monoclonal antibodies and Fc-fusion proteins are reviewed and when used in combination, these strategies can greatly enhance the chance of meeting the drug substance specifications for residual HCP.

TL;DR: An E. coli expression system of rMan i 1 was established, with the potential to be used in immunotherapy against mango allergy or the development of assays for detecting the residues of mango allergens.

TL;DR: The goal of this work was to demonstrate that a modified version of SA SP might be used in different mammalian cells employing the same expression vector, regardless of the transgene copy number.

TL;DR: This study successfully purified, identified, cloned, expressed and characterized a novel α-l-fucosidase, and meanwhile revealed a new multidomain structure of glycoside hydrolase.

TL;DR: The influence of the choice of host strain, induction temperature, duration of induction, and media supplementation with glucose on tau expression in Escherichia coli is reported on.

TL;DR: The novel purification protocol reported here enables efficient isolation of a recombinant GPCR expressed at low titers in host cells and is suitable for preparation of milligram quantities of stable isotope-labelled receptor for high-resolution NMR studies.

TL;DR: The laccase of the basidiomycete Pleurotus pulmonarius may be useful in decolorizing unwanted polyene pigments, for example from the processing of cheese, bakery, desserts, ice cream or coloured casings.

TL;DR: In this article, the enzyme was characterized by thermal stability, pH tolerance and effect of inhibitors, showing optimal collagenolytic activity at 37 °C and pH 9.0 and proved to be a serine protease.

TL;DR: To understand the molecular mechanisms that govern the receptor-ligand interactions, overexpressed the human OR hOR1A1 in a stable tetracycline-inducible HEK293S cell line and revealed that the detergent-solubilized FLAG-rho1D4-tagged hOR 1A1 bound its cognate odorant, dihydrojasmone, with an affinity in the micromolar range.

TL;DR: It is demonstrated that the Car9 tag is functional and TEV protease-excisable when fused to the N-termini of target proteins, and that it supports affinity purification under denaturing conditions, albeit with reduced yields.

TL;DR: Fungi belonging to the genus Fusarium also showed an increase in the endogenous production of ROS when treated with this fraction, and Antimicrobial peptides were also identified in the fruits from other Capsicum species.

TL;DR: It was demonstrated that expression of the EPL complex in the cytoplasm was readily established and that site-specifically mono-alkynated nanobodies can be produced with the same binding properties as the non-modified NbBcII10 expressed in the periplasm.

TL;DR: Development of the high-throughput production methods for bacterial production of recombinant analogue of cytotoxin I from N. oxiana containing additional N-terminal Met-residue (rCTX1) opened new possibilities for structure-function studies of CTX1 and other related three-finger proteins.

TL;DR: Xyn11-1 is the first reported GH11 xylanase isolated from saline-alkali soil, and has excellent tolerance of high pH, high salt concentrations and ethanol, which indicates its great potential for basic research and industrial applications.

TL;DR: Interaction of the protein-peptide pair enforces proximity of the protease and its minimised cleavage sequence, enhancing both catalysis of a sub-optimal site and overcoming steric hindrance, which facilitates the high yield purification of fully native target proteins without recourse to specialised purification columns.

TL;DR: The immobilised enzyme had a higher optimum temperature and greater thermostability than the free enzyme and in immobilised form the enzyme readily catalysed the synthesis of alkyl glucosides.

TL;DR: Recombinant ABP-dHC-cecropin A was successfully expressed, as the product displayed antibacterial and antifungal activities indistinguishable from those of the synthesized protein.

TL;DR: It is determined that, using the GFP fusion substrate bound to Ni-NTA or Strep-tactin agarose, activity of theTEVp5M variant was higher than that of the other TEVp construct, and about 15% higher than the TEvp3M.

TL;DR: Re recombinant human FGF10 (rhFGF10) was expressed in safflower seeds using oilbody-oleosin technology and MTT assays demonstrated that oil bodies expressed oleosin-FGF 10 had a dose-dependent effect on cellular proliferation.

TL;DR: This work shows the production and isolation of a tetrameric and functional native M2 ion channel that will pave the way to structural and functional characterization ofnative M2, conformational antibody development, small molecules compounds screening towards vaccine treatment.

TL;DR: Buffered extra-YNB Glycerol Methanol auto-induction media is developed which not only simplified the protein expression process, but also optimized protein expression levels in P. pastoris and is successfully used to overexpress the target in both MutS and Mut+ strains.

TL;DR: Results indicated that GLOD protein levels and enzyme activity increased with increasing gox copy number, and superior thermo-stability for the enzyme produced in yeast, which further enhances its potential for industrial applications.

TL;DR: A simple yet efficient purification and refolding process using 8 M urea for the solubilization of PsChO3 achieved a high yield of the enzyme in its active form and revealed a distinct binding mode in both FAD and substrate domain, which may explain the enzyme's unusual catalytic behavior.

TL;DR: The codon-optimized hIL-3 gene was cloned under various signal sequences and solubility enhancer fusion tags for its hyper-expression under a strong T7 promoter for its poor expression yield was improved by addressing various upstream bottlenecks in E. coli heterologous system.

TL;DR: Current and emerging options to accelerate the success of protein production in Escherichia coli are described, including innovative molecular biology and expression vectors, small-scale expression screening strategies and the automation of parallel and multidimensional chromatography.