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JournalISSN: 1841-6624

Revista Romana De Medicina De Laborator 

De Gruyter Open
About: Revista Romana De Medicina De Laborator is an academic journal published by De Gruyter Open. The journal publishes majorly in the area(s): Myeloid leukemia & Cancer. It has an ISSN identifier of 1841-6624. It is also open access. Over the lifetime, 370 publications have been published receiving 1032 citations. The journal is also known as: Romanian Journal of Laboratory Medicine.

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Journal ArticleDOI
TL;DR: In this article, the authors report steady sex differences in HIV RNA levels and absolute CD4 cell count in ART-treated HIV-infected patients, a fact that may suggest a reevaluation of current treatment strategies according to sex.
Abstract: Abstract Introduction: Prior studies assessing sex differences correlated with the levels of human immunodeficiency virus (HIV) RNA and absolute CD4 cell count in adults and children, treated or untreated with antiretroviral (ARV) therapy presented conflicting results. Objective: To assess comparative HIV RNA levels and absolute CD4 cell count in men and women from a large cohort of HIV-infected long term survivors patients. Methods: 462 HIV infected patients were analyzed cross-sectionally and longitudinally after being split into three groups: 156 naïve deceased patients, median age at death 10 years, 197 ARV treated patients, median age 17 years and 109 ARV treated patients, median age 23 years followed up until 2011. HIV RNA and absolute CD4 cell count were measured in all patients enrolled in the study. Results: In cross-sectional analysis of 156 naïve patients HIV RNA median levels were lower in females comparing to males, 4.95 vs. 5.73 HIV RNA log10 (copies/ml). Female absolute CD4 cell count was slightly higher, (median 97 vs. 65.5 cells/μL; P = 0.0001). Cross-sectional analysis of 197 ARV treated patients showed a lower log10 HIV RNA level in females compared to males, (P=0.0001), and also lower median CD4 count values in women, 336 cells/μL vs 456 cells/μL in men, P=.0001. Longitudinal analysis revealed statistically significant results: mean log viral loads were lower in females (F=13.90, P= 0.0009) and absolute CD4 cell count was lower in malse (F=16.72, P<0.0001), almost across all tested ages. Conclusion: We report steady sex differences in HIV RNA levels and absolute CD4 cell count in ART-treated HIV-infected patients, a fact that may suggest a reevaluation of our current treatment strategies according to sex. Rezumat Introducere. Studiile anterioare care evaluează diferenţele în funcţie de sex ale încărcăturii virale HIV şi ale numărului absolut de celule CD4 la adulţi şi copii trataţi sau netrataţi cu terapie antiretrovirală au prezentat rezultate contradictorii. Obiectiv: Evaluarea comparativă a valorilor incărcăturii virale ARN HIV şi a numărului absolut de celule CD4 la bărbaţi şi femei, într-un lot de pacienţi infectaţi cu HIV, supravieţuitori de lungă durată. Metode: 462 pacienţi infectaţi cu HIV au fost analizaţi prin studii transversale şi longitudinale, după împărţirea în trei grupe: 156 pacienţi netrataţi anterior şi decedaţi, cu vârsta medie la deces 10 ani; 197 pacienţi trataţi cu antiretrovirale, de vârstă medie 17 ani şi 109 pacienţi trataţi cu antiretrovirale, de vârstă medie 23 ani, care au fost monitorizaţi până în anul 2011. Incărcătura virală ARN HIV şi numărul absolut de celule CD4 au fost determinate la toţi pacienţii înrolaţi în studiu. Rezultate: Analiza transversală a celor 156 pacienţi netrataţi anterior a arătat că nivelurile medii ale încărcăturii virale ARN HIV au fost mai mici la femei comparativ cu bărbaţii, 4.95 vs 5.73 log10 copii/ml. Valoarea numărului absolut de celule CD4 a fost mai mare la femei, (valoare mediană: 97 vs 65.5 celule/μL, P = 0.0001). Analiza transversală a celor 197 pacienţi trataţi cu aniretrovirale a arătat o diferenţă de 1 log10 ARN HIV între femei şi bărbaţi, cu un nivel mai scăzut al încărcăturii virale la femei, (P = 0.0001), de asemenea valoarea mediană a numărului de celule CD4 a fost mai scăzută la femei, 336 celule/μL vs 456 celule/μL la bărbaţi, P = 0.0001. Analiza longitudinală a arătat rezultate semnificative statistic: valorile mediane ale încărcăturii virale erau mai scăzute la femei (F = 13.90, P = 0.0009) şi valorile numărului absolut de celule CD4 erau mai scăzute la bărbaţi (F = 16.72, P<.0001), aproape la toate categoriile de vârstă testate. Concluzie: Raportăm diferenţe constante în funcţie de sex, atât pentru nivelurile încărcăturii virale ARN HIV, cât şi pentru valorile numărului absolut de celule CD4, la pacienţii infecţati cu HIV şi trataţi cu terapie antiretrovirală, fapt care ar putea sugera o reevaluare a strategiilor actuale de tratament în funcţie de sex.

20 citations

Journal ArticleDOI
TL;DR: There is no evidence of a direct role of IL-6 -174 G/C gene polymorphism in sepsis risk and outcome, and circulating IL- 6 levels were significantly higher in the septic shock subgroup and among patients with GG genotypes of both studied polymorphisms.
Abstract: Abstract Objectives: The goal of the study was to investigate the correlations between the interleukin-6 IL-6 -174 G/C and IL-6 -572 G/C gene polymorphisms and sepsis risk and severity in adult ICU patients. Materials and Methods: We prospectively assessed 107 septic patients and divided them into two subgroups: organ dysfunction-free sepsis subgroup S (n=60) and septic shock subgroup SS (n=47). A control group of 96 healthy individuals was included. Both patients and controls underwent IL-6 -174 G/C and -572 G/C genotyping and circulating IL-6 in the study group which were measured from samples taken in the first day of sepsis diagnosis. Results: No differences in the genotype frequencies of the two polymorphisms between study and control groups were identified. The GC genotype and C allele of IL-6 -572 G/C gene polymorphism was statistically significant more frequent in the organ dysfunction-free subgroup (p=0.01, p=0.004 respectively). No statistically significant differences for the IL-6 -174 G/C gene polymorphism were found between the two sepsis subgroups. Circulating IL-6 levels were significantly higher in the septic shock subgroup and among patients with GG genotypes of both studied polymorphisms. Conclusion: We underline the possible role of IL-6 -572 G/C as a marker of severe evolution. There is no evidence of a direct role of IL-6 -174 G/C gene polymorphism in sepsis risk and outcome. Il-6 levels are correlated with sepsis severity but not with variant genotype of investigated IL-6 gene polymorphisms.

16 citations

Journal ArticleDOI
TL;DR: There is a connection between obesity and kidney disease and several cardiometabolic factors, and high-fat calorie consumption as a result of modern diet leads to increased concentration of polyunsaturated fatty acids with higher omega-6:omega-3 ratio.
Abstract: Obesity represents a major social problem, especially in the developed countries, with growing incidence in adults and children, and it is associated with low-grade inflammation in the white adipose tissue (1). According to recent data, there are around 500 million adult obese individuals worldwide. The definition of obesity is based on the body mass index value (BMI), exceeding 30 kg/m2 (2). In the epidemiological study PREDATORR performed on 2,681 Romanian subjects, Popa and his collaborators found that obesity was present in 34.70% of the people included in the study group aged 20 to 79 years, while the metabolic syndrome was 38.50%. The abdominal obesity represented 73.90 % whereas the obesity rate adjusted for age and sex was 31.90 %. They concluded that there is a connection between obesity and kidney disease and several cardiometabolic factors (3). In the same study, glucose regulation disorders were detected in 28.1% of the subjects, leading to prediabetes, diagnosed or undiagnosed diabetes mellitus (4). In contrast with our ancestors’ protein-rich diet, nowadays the majority of people have a diet rich in carbohydrates and lipids. These individuals are very likely to gain weight, especially in the case of a sedentary lifestyle, which is also very common in countries with averageand high income (5). This mechanism may contribute to the development of insulin-resistance (6), another population-level problem regarding the increasing incidence of type 2 diabetes. The high-fat calorie consumption as a result of modern diet leads to increased concentration of polyunsaturated fatty acids (PUFA) with higher omega-6:omega-3 ratio, which can be precursors for several mediators, maintaining a chronic inflammatory state (7). This low-grade inflammation can trigger several chronic diseases such as obesity and osteoporosis (8). Recent research data revealed changes in the human brain due to obesity-related

16 citations

Journal ArticleDOI
TL;DR: Bacterial phenotype (highly adaptable bacteria, biofilm formation) and structure may drastically affect the responsiveness to the antimicrobial activity of alcohols, leading to higher bactericidal than inhibitory concentrations.
Abstract: Abstract Alcohol based solutions are among the most convenient and wide spread aid in the prevention of nosocomial infections. The current study followed the efficacy of several types and isomers of alcohols on different bacterial species. Seven alcohols (ethyl, n-propyl, iso-propyl, n-butyl, iso-butyl, tert-butyl alcohol, and ethylene glycol) were used to evaluate their minimal inhibitory and bactericidal effects by microdilution method on bacteria that express many phenotypical characteristics: different cell-wall structure (Gram positive/negative bacteria), capsule production (Klebsiella pneumoniae), antibiotic resistance (MRSA vs MSSA) or high environmental adaptability (Pseudomonas aeruginosa). Results: The best inhibitory effect was noticed for n-propyl, followed by iso-propyl, n-butyl, and iso-butyl alcohols with equal values. Ethylene glycol was the most inefficient alcohol on all bacteria. In K. pneumoniae and P. aeruginosa, the bactericidal concentrations were higher than the inhibitory one, and to a level similar to that encountered for most of the Gram-positive bacteria. Among Gram-positive cocci, E. faecalis presented the lowest susceptibility to alcohols. Conclusions: All alcohols presented good effect on bacteria, even in low concentrations. Compared to ethanol as standard, there are better alternatives that can be used as antimicrobials, namely longer-chain alcohols such as propyl or butyric alcohols and their iso- isomers. Ethylene glycol should be avoided, due to its toxicity hazard and low antimicrobial efficacy. Bacterial phenotype (highly adaptable bacteria, biofilm formation) and structure (cell wall structure, presence of capsule) may drastically affect the responsiveness to the antimicrobial activity of alcohols, leading to higher bactericidal than inhibitory concentrations.

16 citations

Journal ArticleDOI
TL;DR: In this paper, the antioxidant potential of crude extract and its fractions from Brassica rapa L. fruit part was tested for glutathione peroxidase (GPx), superoxide dismutase (SOD) enzymes and total antioxidant status (TAS) in blood samples.
Abstract: Abstract Research on antioxidant potential from vegetables is increasingly focused on their effects on human health. However, relatively little work has been done to investigate the antioxidant effect of crude extract and/or different fractions from Brassica rapa L. In the present study, the antioxidant potential of crude extract and its fractions from Brassica rapa L. fruit part was tested for glutathione peroxidase (GPx), superoxide dismutase (SOD) enzymes and total antioxidant status (TAS) in blood samples. Our results reveal the fact that crude extract and each analyzed fraction (i.e. aqueous, ethyl acetate and chloroform) showed a concentration dependent effects on GPx, SOD and TAS in respect with saline solution (0.9% NaCl) used as negative control and vitamin C, as positive control. Therefore, GPx levels showed a highest value in crude extract and chloroform fraction (6981 U/L both at 10 mg/ml), SOD levels showed the same results in aqueous and ethyl acetate fractions (220 U/ml both at 10 mg/ml) and TAS in crude extract and all three fractions (i.e. aqueous, ethyl acetate and chloroform, 1.68 mmol/L at 10 mg/ml for all three fractions) in respect with saline solution (p<0.05). Furthermore, vitamin C showed the highest values on all three analyzed enzymes (8769 U/L for GPx, 223 U/ml for SOD and 1.8 mmol/L for TAS at 100 μg/ml). Our investigations have been proved to be promising in terms of future potential applications of crude extract and its fractions as components in a range of phytochemicals composition and/or different pharmaceutical usage, owing to their antioxidant potential. Rezumat Cercetările asupra potenţialului antioxidant din legume prezintă o importanţă crescută a efectelor sale asupra sănătaţii umane. Cu toate acestea, puţine studii au fost realizate pentru a investiga efectul antioxidant al extractului crud şi/sau a diferitelor fracţiuni din Brassica rapa L. În studiul de faţă, potenţialul antioxidant al extractului crud şi a fracţiunilor acestuia din fructul Brassica rapa L. a fost testat pentru enzimele glutation peroxidaza (GPx), superoxid dismutaza (SOD) şi statusul total antioxidant (STA) în probe de sânge. Rezultatele noastre au arătat faptul că atât extractul crud cât şi fiecare fracţiune analizată (apoasă, de acetat de etil şi cloroformică) au arătat efecte dependente de concentraţie asupra GPx, SOD şi STA în comparaţie cu soluţia salină (0,9% NaCl) folosită ca şi control negativ şi vitamina C, ca şi control pozitiv. Prin urmare, nivelurile GPx au prezentat valori crescute în extractul crud şi fracţiunea cloroformică (6981 U/L ambele la 10 mg/ml), nivelurile SOD au prezentat aceleaşi rezultate în fracţiunea apoasă şi cea de acetat de etil (220 U/ml ambele la 10 mg/ml) şi STA în extractul crud şi toate cele trei fracţiuni (apoasă, de acetat de etil şi cloroformică; 1,68 mmol/L la 10 mg/ml pentru toate fracţiunile) în comparaţie cu soluţia salină (p<0,05). Mai mult, vitamina C a prezentat cele mai mari valori asupra celor 3 enzime analizate (8769 U/L pentru PGx, 223U/ml pentru SOD şi 1,8 mmol/L pentru STA la 100 μg/ml). Investigaţiile noastre se dovedesc a fi promiţătoare în ceea ce privesc viitoarele aplicabilităţi ale extractului crud şi fracţiunile acestuia ca şi componenţi în diferite preparate fitochimice şi/sau de uz farmaceutic datorită potenţialului lor antioxidant.

15 citations

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No. of papers from the Journal in previous years
YearPapers
20231
20224
202138
202037
201941
201847