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Showing papers in "Systematic and Applied Microbiology in 1995"


Journal ArticleDOI
TL;DR: A 16S rRNA-targeted oligonucleotide probe (NEU) specific for some representatives of the lithoautotrophic ammonia-oxidizing bacteria was developed based on comparative sequence analysis and showed promise in detecting dense cell clusters exclusively in those samples originating from sewage treatment plants with stable nitrification.

541 citations




Journal ArticleDOI
TL;DR: The transformation of sucrose into glucose, fructose, gluconic acid, ethanol, and acetic acid was determined during a 60 day tea fungus fermentation, implying that all major fermentation products have been accounted for.

137 citations


Journal ArticleDOI
TL;DR: In this paper, a coccoid thermoacidophilic metal-mobilizing archaeum has been isolated from a smoldering slag heap of a uranium mine in Thuringen, Germany.

125 citations



Journal ArticleDOI
TL;DR: It was found that the gallate degrading strains of S. bovis should be re-assigned to a new species, for which the name Streptococcus gallolyticus sp.

115 citations


Journal ArticleDOI
TL;DR: In this paper, the 16S / 23S rRNA spacer region was amplified with three major products: 270, 400, and 430 nucleotides, with the corresponding bands in the B. cereus group at 250, 430, and 480.

110 citations


Journal ArticleDOI
TL;DR: The approximate time of divergence between the various species of Rickettsia, estimated from the bacterial 16S rRNA molecular clock, coincides with the approximate divergence time of the hard body ticks which are the arthropod hosts of manyRickettsia .

105 citations


Journal ArticleDOI
TL;DR: The results of this phylogenetic analysis confirm the previously proposed emendation of the genus classification within these bacteria, and suggest that the morphological traits have evolved recently, and that 16S ribosomal RNA analysis alone is not sufficient to determine the evolutionary relationship between them.

103 citations


Journal ArticleDOI
TL;DR: The new methods based on PCR amplification and sequencing of 16S rRNA genes from DNA samples extracted directly from the environment allow the description of microbial diversity in natural ecosystems without the need for cultivation to be applied to an extreme environment presumed to have very low diversity.

Journal ArticleDOI
TL;DR: On the basis of chemotaxonomic data, biochemical and physiological properties, and whole cell protein profiles, the four pentachlorophenol-degrading strains Arthrobacter sp.

Journal ArticleDOI
TL;DR: The majority (91%) of 260 isolates initially identified as Vibrio anguillarum, that were obtained from a wide range of hosts, habitats and geographical locations, were recovered in a single cluster based on the ribotype and were pathogenic to Atlantic salmon.

Journal ArticleDOI
TL;DR: Gram-positive, straight filamentous bacterium “ Microthrix parvicella” is isolated from an activated sludge sewage treatment plant in Australia by using micromanipulation techniques and phylogenetically placed as a deep branching member of the actinomycetes subphylum.

Journal ArticleDOI
TL;DR: In this article, the phylogeny of bacteria that degrade a tannin-protein complex (T-PC) isolated from the fecal biota of koala was investigated.

Journal ArticleDOI
TL;DR: The isolation of a halotolerant, Fe(III)-reducing, Gram-negative bacterium from surface sediments of the Sippewisset marsh in Woodshole (USA) revealed that the newly isolated strain is closely related to the previously isolated strain BrY.

Journal ArticleDOI
TL;DR: In this paper, a genus-specific PCR assay was created for the detection of all Arcobacter strains and three speciesspecific PCR assays were developed for the discrimination among A. cryaerophilus, A. butzleri and A. skirrowii.

Journal ArticleDOI
TL;DR: A phylogenetic tree reflecting the relationships among proteobacteria was reconstructed based on 23S rRNA sequence comparison, similar to that of a tree based on an equivalent 16S r RNA sequence data set.

Journal ArticleDOI
TL;DR: Experiments showed that the obtained sequences were derived from the dominant propionate-oxidizing bacteria in the enrichment cultures, which are closely related to Syntrophobacter wolinii.

Journal ArticleDOI
TL;DR: In this article, four pentachlorophenol (PCP)-degrading bacterial strains: Arthrobacter sp. ATCC 33790, Pseudomonas sp. SR3, Flavobacterium sp. AtCC 39723, and Pseudomanas s. RA2 were analyzed by 16S rRNA gene sequence comparisons, REP/ERIC PCR fingerprinting of genomic DNA and serological typing by reaction to antisera prepared against each strain.

Journal ArticleDOI
TL;DR: Principal coordinate analysis of fatty acid data have shown that the majority of the type strains and marine species, identified as Alteromonas sp.

Journal ArticleDOI
TL;DR: In comparison with nuclear DNA/DNA hybridization or electrophoretic karyotyping, RAPD-PCR anaylsis turned out to be a simple and reliable method to separate Saccharomyces species at the genotypic level.

Journal ArticleDOI
TL;DR: A. Facilis-A. aminolytica and A. facilis was transferred to a new genus, Acidocella gen. nov as mentioned in this paper.

Journal ArticleDOI
TL;DR: The phylogenetic diversity of magnetic cocci was found to exceed the variety of distinguishable morphotypes and sequence differences between 16S rDNAs of closely related magnetotactic cocci were indeed present in the 15S rRNA and not due to sequencing or PCR artifacts.

Journal ArticleDOI
TL;DR: Thioploca and Beggiatoa constitute a new phylogenetic lineage within the gamma-sub division of the proteobacteria, showing similar genetic diversity as other major groups of gamma- or beta-subdivision sulfur oxidizers.

Journal ArticleDOI
TL;DR: The new isolates appear to be members of the genus Bacillus but 16s rDNA sequence analysis and sodium dodecyl sulfate-polyacrylamide gel electrophoresis comparisons of whole cell protein profiles, as well as nutritional, biochemical and morphological traits indicated the new isolate are not members of any validly published Bacillus species.

Journal ArticleDOI
TL;DR: The two Ophiostoma species separated harbour isolates predominantly grown on spruce on the one hand and gathered isolates from oak and probably other hardwoods on the other hand and the name O. quercus must be reinstated.

Journal ArticleDOI
TL;DR: In this paper, restriction fragment length polymorphisms (RFLPs) were distinguished, forming two main clusters: one contained all Xanthomonas species, the other contained strains of Stenotrophomonas spp.

Journal ArticleDOI
TL;DR: In this paper, the syntrophically benzoate-oxidizing anaerobic bacterium Syntrophus bustvellii was grown in pure culture in a defined mineral medium with crotonate as sole substrate, which was fermented stoichiometrically to acetate and butyrate.

Journal ArticleDOI
TL;DR: Although highly atypical strains proved difficult to classify, the value of numerical analysis of phenotypic characters for the classification and identification of campylobacteria has considerable potential for accurately differentiating members of this taxonomically complex and economically important group of bacteria.