Thai Journal of Agricultural Science 

About: Thai Journal of Agricultural Science is an academic journal. The journal publishes majorly in the area(s): Crop yield & Germination. It has an ISSN identifier of 0049-3589. Over the lifetime, 306 publications have been published receiving 1181 citations.

Polyploid induction by colchicine treatments and plant regeneration of Dendrobium chrysotoxum.

Abstract: The purpose of this research was to investigate the effect of colchicines concentration and duration time to polyploid induction and plant regeneration of Dendrobium chrysotoxum L. The method was conducted by inclusion of colchicine into semi-solid VW medium. Protocorm like bodies (PLBs) of diploid D. chrysotoxum were treated with 0, 0.01, 0.02, 0.03, 0.04, and 0.05% colchicines (w/v) for 1, 2, 3, 4 and 5 days. The most effective treatment was 0.04% colchicine for 1 day which resulted in about 84% surviving PLBs and with 47 % of tetraploid orchids, as measured by flow cytometry. The treated PLBs were cultured on the same medium supplemented with 0, 0.5 and 1 mg L -1 NAA and 0, 0.5 and 1 mg L -1 BA for plant regeneration. Treated PLBs with 0.01% and 0.02% colchicines, the highest number of proliferated shoot (2.36 per explants and 2.44 per explant respectively) was obtained from 1 mg L -1 NAA and 0.5 mg L -1 BA. In the treatment with 0.03% and 0.04% colchicines, the highest number of proliferated shoot (3.40 per explants and 4.35 per explants respectively) was obtained from the culture media supplemented only with 0.5 mg L -1 BA and 1 mg L -1 BA .

SSR markers linking to seed traits and total oil content in soybean.

Abstract: Soybean is an economic crop used as a main source of vegetable oil. Quality and quantity of oil in soybean seed is important as a raw material determining manufacturing cost. The markers associating with oil content are useful in selection for soybean lines with high oil. The aim of this research was to develop molecular markers linking to genes controlling seed traits and total oil content in soybean. An F2:3 population comprising 186 families was developed from a cross between Pak Chong 2 and Laos 7122. The population was genotyped by 159 polymorphic SSR markers, and seeds were determined for oil content by hexane extraction method. QTL analysis was done by a simple regression method and composite interval mapping. Finally, 138 SSR markers were grouped into 30 linkage groups covering 1,921.1 cM of soybean genome. Twentyone polymorphic markers remained unlinked. There were 10 QTLs located on linkage groups A1, C2, E, and G that found associating with nodes per plant, seed length, seed width, hundred seed weight and total oil content.

Development of a bioassay to study anthracnose infection of Capsicum chinense Jacq. fruit caused by Colletotrichum capsici

Abstract: A fruit inoculation bioassay was developed for chinense chilli by Colletotrichum capsici. Four fruit green, color turning and ripe red; and thr:ee ' ' . were applied to an anthracnose resulted in anthracnose symptoms inoculation, while the drop method after inoculation. The green more mature ripe red fruit symptoms. All the disease area under the disease ; infection of Capsicum immature green, mature .injection and wound/drop; Injection and wound/drop methods stages as early as 3-5 days after -of anthracnose within 9 days , more susceptible to anthracnose than the not affect the development of anthracnose ; lesion length, lesion width, lesion area and correlated indicating that any of the disease 1 for resistance. Key inoculation method sp.) is an economically crop of Thailand with -constraint to preand post-harvested chilli fruits. In Thailand, C. annuum is the major species of commercial chilli grown however, C. chinense is also grown for its high capsicin content and disease resistance. Anthracnose of chilli species is caused by either Co//etotrichum capsici (Syd.) E.J. Butler & Bisby or C. g/oeosporioides (Penz.) Penz. & Sacc. in Penz. C. capsici has also been reported to infect a wide range of legume species (Pring et al., 1995). Chilli important Fruit maturity stage has been shown to be important in the infection and colonisation of C. annuum chilli fruit by C. gloeosporioides with red fruit being more resistant than green fruit (Kim et al., 2001,2002; Oh et al., 1999a, 1999b). However, (Manandhar et al., 1995) reported that both ripe and green chilli fruits reacted the same to either C. capsici or C. gloeosporioides. Three laboratory inoculation methods (injection, drop and wound/drop) for studying anthracnose diseases of C. annuum chilli hav~ been developed. The injection and drop methods were developed at