Showing papers in "The Korean Journal of Thoracic and Cardiovascular Surgery in 1997"

New Treatment Method for Reexpansion Pulmonary Edema: Differential Lung Ventilation.

Abstract: Reexpansion pulmonary edema (REPE) is an iatrogenic complication that develops in a lung rapidly reinflated after varied periods of collapse. Though infrequent, it is often lethal. Measures to prevent REPE are well known and mild cases may be reversed with conventional ventilator therapy and medication; however, there is no definitive treatment for this condition in its severest forms. Asynchronous differential lung ventilation is a relatively new therapeutic modality for preoperative, intraoperative, or postoperative treatment of respiratory failure secondary to ventilation-perfusion mismatch. Successful treatment of severe REPE in a 29-year-old male patient is described, using asynchronous differential lung ventilation.

기관지내 지방종 -체험 1례-

Abstract: 기관지내 지방종은 주로 기관지 근위부에서 발병하면서, 그 원위부의 폐실질에 비가역적 손상을 입히는 아주 드문 질환이다. 이는 조직학적으로는 양성종양이다. 그러나 진단 및 치료가 늦을 경우에는 비가역적 폐실질 손상 및 기관지확장증 등을 유발시킬 수 있다. 가능하다면 가장 좋은 치료방법은 기관지 내시경을 이용한 종양 제거술이다. 그러나 내시 경을 이용하여 종양 제거가 불가능하거나, 진단이 불명확한 경우에는 폐엽절제술 또는 전폐적출술 등을 포함한 개흉술이 필요한 경우가 많은데, 이는 이들 대부분이 폭넓은 폐실질 손상을 동반하고 있기 때문이다. 우리는 기관지 내경을 완전히 막으면서. 그 원위부에 비가역적 손상을 일으킨 기관지내 지방종 1례를 경험하였기에 이를 일반적인 기관지내 지방종의 임상증상, 진단 방법, 그리고 치료방법 등과 함께 보고하는 바이다. 【Endobronchial lipomas Are rare lesions that usually obstruct a major bronchus and cause irreversible pulmonAry damage distally. They are histologically benign tumors. But they can produce pulmonary damage or irreversible bronchiectasis if dignoses or tr atments are delayed. Whenever possible, the treatment of choice is resection by means of bronchoscopy. If endoscopic removal is not possible or lf the nature of the tumor is unclear, surgery is necessary. with lobectomy or pneumonectomy being required in most cases due to the extensively damaged pulmonary parenchyma . We present a case of endobronchial lipoma causing bronchial obstruction and peripheral organizing pneumonia with its clinical features. diagnosis find treatment methods.】

폐암환자의 암조직내 CYFRA 21-1과 Epidermal Growth Factor Receptor의 측정치에 대한 연구

Abstract: CYFRA 21-1은 폐암중에서 편평상피성 암세포의 세포질에 존재하는 세포각질 분절 19의 분절들로서 암세 포가 파괴되거나 분해시 혈중내로 유리되는 것으로 특징적인 2개의 단일클론성의 항체인 KS 19-1과 BM 19-21로서 면역 방사계수검사를 이용하면 혈청내에 용해된 량을 정량할 수 있다. 암세포의 세포벽에 존재하는 EGF-R과 EGF에 대하여 관심이 집중되고 있다. EGF-R의 존재는 클론성 비소세포암 세포계열을 조사한 결과 4종의 비소세포암들은 EGF-R을 발현한다고 밝혀졌다. 그러나 현재의 검사법으로는 EGF 검출이 어려워서 EGF보다 TCF-Q의 역할에 초점이 모여지고 있다. 폐암세포에 EGF-R의 존재는 자가분비나 부분비성 성장기전이 작용된다는 것을 시사한다. 아울러 정상인 의 혈청과 소변에서 검출이 되며, 이러한 사실을 종합해 본 결과 EGF-R은 폐암의 발달과 진행에 중요한 역 할을 할 것으로 추정된다. 폐암으로 확진된 30례의 환자를 연구 대상으로 하고, 개흉수술로 적출한 표본을 주병소와 이행부위 그리 고 대조부위로 구분하여 조직 절편을 약 5 m3크기로각각 잘라서 액화 질소에 급속 냉동 보관을 하였다. 냉 동 보관한 조직 절편을 조직마쇄 藪 $[$ \ulcorner마쇄시킨후 원심분리기에서 상층액을 일정량 채취하여 방사선면역 분석법으로 CYFRA 21-1과 EGF-R 정량검사를 시행하였으며, 그 결과를 조직학적 분류와 병기에 따른 분류 로 상호 비교 분석하였다. 이상과 같은 연구결과로 아래와 같은 요점들을 발견하였다. 1. 암 이행부위에서는 악성화를 나타내는 경향이 더욱 활발하여 세포질성분의 부족으로 CYFRA 21-1의 농도 는 낮게 나타났다. CYFRA 21-1의 농도는 암이행부위에서 가장 낮았고, 병기가 증가할수록 증가하였다. 대 조조직에서는 세포질 성분이 풍부하여 주병변부위보다도 CYFRA 21-1의 농도가 높게 나왔다. 2. EGF-R의 농도는 주병변부위에서 가장 높게 나왔고, 편평세포암에서 보다는 선암에서 높았고, 병기별로는 1, 1띠에서는 이행부위가 111, IV기에섞는 주병변부위가 높게 나왔다. EGF-R의 농도는 대조조직보다는 암 주병변부위로 갈수록 증가하였다. 3. CYFBLt 21-1은 세포질성분이며, EGF-R은 세포벽 성분으로서 두 물질사이에 상관관계가 없었다. 결론적으로 현재까지 CYFRA 21-1은 혈청 내에서만 주로 연구되어져 왔으며 비소세포암 중에서 특히 편 평세포암종에서 의미있게 증가한다고 하였다. 그러나,. CYFRA 21-1의 조직과 혈청 내의 정량치가 뜻하는 의 미는 서로 달랐으며, 암조직내에서 대조조직내보다 CYFRA 21-1 치가 더 낮게 나온 것은 암세포내 에서는 세 포질 성분의 고갈로 인한 것으로 추정되며 암세포의 활동성과는 무관한 것으로 판단된다. EGF-R은 세포벽내에 존재하는 수용체로서 암세포의 증식에 따라 증가하는 양상을 보이며 대조조직보다는 암세포에서 유의한 증가를 보이는 것은 종양 증식과 암표지자로서 의의가 있는 것으로 판단된다. 【CYPRA 21-1 is known to be a cytokeratin 19 fragment, and it can be detected by using two specific monoclonal antibodies (KS 19-1 and BM 19-21) and can be clinically applied as a useful circulating tumor marker The epidermal growth factor receptor (EGF-R) expression was evaluated and characterized by its tyrosine protein kinase activity and by its ligand-stimulated autophosphorylation, a property shared with other peptide growth factor receptors. Autocrine or para'urine action was initiated by a growth factor, or by a transforming growth factor o, which had an extensive homology with EGP and which also stimulated tyrosine kinase activity on the EGF-R. The CYFRA 21-1 and the EGF-R levels in 30 patients with primary lung tumors were investigated. There were 24 patients with squamous cell carcinomas and 6 patients with adenocarcinomas. Specimen 5 mm3 in size were sampled at three different locations ; the main lesion, the boundary between the lesion and the unaffected tissue, and the unaffected tissue of the patients. The results were as follows 1. The CYPRA 21-1 concentration in the cancer boundary, the most malignant region,(348.6 : 89.9 ng/ml) was the lowest value. The CYFRA 21-1 concentration in unaffected tissue,(718.4 $\pm$ 77.8 ng/ml) was higher than that in the main lesion. which had intact cellularity. 2. The EGF-R concentration in the main lesion was higher than that in the unaffected tissue, and the EGF-R concentration in a squamous cell cacinoma was higher than that in an adenocarcinoma. also, the EGF-R concentration in the cancer b undary was highest at stage 1, ll. The EGF-R concentration was higher in the main cancer lesion that in the unaffected tissue at stage 111, IV. 3. The CYFRA 21-1 was a cytoplasmic skeleton and the EGF-R was a cell-wall component; there was no correlation. In conclusion, CYFRA 21-1 was abundant in the cytoplasm but had a higher concentration in the unaffected tissue than in the main cancer lesion. The CYFRA 21-1 concentration of the tissue did not reflect the amount of cancer activity, the EGP-R was located in the cell membrane, the level of tissue that reflects cancer activity, so the main cancer lesion had a higher concentration than the unaffected tissue. CYFRA 21-1 is not a useful tumor maker at the tissue level. Because the EGF-R concentration re(looted the cancer activity, its a useful tumor marker for lung cancer.】

Flow Cytometric Analysis of Endothelial Cell Viability in Arterial Allograft

Abstract: Arterial allografts have known advantages over prosthetic vascular conduit for treatment of heart valvular disease, congenital heart disease, and aortic disease. Cell viability may play a role in determining the long-term outcome of allografts. Endothelial cell is one important part in determining the allograft viability. To evaluate the viability of endothelial cells using current allograft preservation techniques, porcine heart valve leaflets and arterial wall were subjected to collagenase digestion. Single endothelial cell suspension was labeled withGriffonia simplicifolia agglutinin-fluorescein isothiocyanate (GSA-FITC), a vascular endothelial cell specific marker. The cell suspension was washed and incubated with propidium iodide (PI), which does not bind with viable cells. Endothelial cell viability was evaluated by calculating the percentage of the GSA-FITC(+) and PI(−) groups using flowcytometric analysis. For sterilization, allografts were treated with 4°C antibiotic solution for 24 hours. After this, half of the allografts were stored in 4°C RPMI 1640 with HEPES buffer culture medium with 10% fetal bovine serum for 1–14 days (Group I). The other half of the allografts was cryopreserved with a currently used technique (Group II). During the procurement and sterilization, 22.8% and 24.4% of endothelial cell viability declined, respectively. In Group I, 11.9% of endothelial cell viability steadily declined further during 14 days of storage. In Group II, 13.7% of endothelial cell viability declined. These results show that the largest loss of endothelial cell viability occurs during the initial process. After 14 days of storage under 4°C nutrient medium or cryopreservation, about 40% of endothelial cell viability is maintained. There were no differences among the endothelial cell viability from aortic valve leaflet, pulmonic valve leaflets, aortic wall, and pulmonic wall.

판막 치환 수술 후 생긴 판막 주위 누출과 관련된 대동맥동류의 심실 중격 박리

Abstract: Purpose All staff, parents, volunteers, contractors, clergy and other members at Our Lady’s School are expected to actively contribute to a school culture that respects the dignity of its members and affirms the Gospel values of love, care for others, compassion and justice. They are required to observe child-safe principles and expectations for appropriate behaviour towards and in the company of children, as noted below.