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Showing papers in "Theriogenology in 1984"


Journal ArticleDOI
TL;DR: The results indicated that the follicles which ovulated originated from the population present when the superovulation treatment was initiated.

357 citations


Journal ArticleDOI
S.P. Leibo1
TL;DR: A method has been developed that permits bovine embryos to be diluted out of the protective solution within the plastic straw in which the embryos were originally rrozen and thawed.

187 citations



Journal ArticleDOI
TL;DR: Serum gonadotropin concentrations were high and variable and fluctuated episodically in short and long term ovariectomized ewes and with or without the influence of supplemental estradiol-17beta.

170 citations


Journal ArticleDOI
TL;DR: Evidence for variability in LH and FSH activity among equine chorionic gonadotrophin, porcine FSH and human menopausal gonadOTrophins is presented and lower ratios of FSH LH activity appeared to reduce ovulatory success in rats, and addition of PLH to FSH-P reduced superovulation in crossbred cows.

159 citations


Journal ArticleDOI
TL;DR: Real-time B-mode ultrasonography was used to evaluate uterine changes associated with the estrous cycle in 22 ovulatory periods in 12 nulliparous heifers and the presence of an embryo within the embryonic vesicle was confirmed by observing an echogenic area with rhythmic pulsations (heartbeat).

154 citations


Journal ArticleDOI
TL;DR: After three cycles, pregnancy rate at 30 days postovulation was lower and mares fed to high body condition were allowed to gain weight after foaling to attain a high level of body condition by 90 days postpartum.

154 citations


Journal ArticleDOI
TL;DR: The best procedure for concentrating sperm was diluting semen to 50 x 10(6) sperm/ml with a citrate-based centrifugation medium at 20 degrees C and centrifuging at 400 x g for 15 min, and a new procedure for processing, freezing and thawing semen evolved.

153 citations


Journal ArticleDOI
TL;DR: Equations which model the influx and efflux of cryoprotectants in cells can be used to calculate the optimum and most practical addition and removal method, however, the equations require the permeability coefficient of the cryop Rotectant, a quantity that has only experimentally determined for a few of the developmental stages of two species.

147 citations


Journal ArticleDOI
TL;DR: This technique provides a method for routine production of identical twin calves in which more calves are produced per two demi-embryos than per whole embryo.

123 citations


Journal ArticleDOI
TL;DR: The results demonstrated that ultrasound technology provides a noninvasive form of visual access to the uterus to evaluate normal, morphological changes and to detect and study certain pathological processes.

Journal ArticleDOI
TL;DR: Significant correlations were found between the duration of elevated PGF(2alpha) metabolite levels and the time required for completion of uterine involution and the occurrence of the first ovulation followed by a normal luteal phase length in the 29 cows.

Journal ArticleDOI
D.H. Douglas-Hamilton, R. Osol1, G. Osol1, D. Driscoll, H. Noble 
TL;DR: A field trial of artificial insemination in horses with transproted, chilled semen was conducted using a specially designed container which permitted a controlled, slow initial rate of cooling and maintenance of a final temperature of 4 degrees -6 degrees C for more than 36 hrs.

Journal ArticleDOI
TL;DR: The reproductive endocrinology of Bosindicus cattle differs from that of Bostaurus breeds; the estrus is shorter and less intense and occurs late in relation to an estrogen stimulus and the fertility is lower during the late fall and winter months.

Journal ArticleDOI
TL;DR: The rabbit oviduct is still useful as an experimental tool in the manipulation of embryos from the domestic species as it has been used for the short term storage of agar cylinders and has been found to be unsuitable because of the high rate of degeneration of Agar chips.

Journal ArticleDOI
TL;DR: Intrauterine mobility patterns of the embryonic vesicle were characterized on Days 9 to 17 after ovulation in pony mares using real-time ultrasonography and mobility was determined by the number of times the vesicles changed locations during successive examinations.

Journal ArticleDOI
TL;DR: Differences seemed to be caused by the presence of many 2- to 5-mm follicles during early diestrus, initiation of growth of large follicles at mid-cycle, selective accelerated growth of an ovulatory follicle beginning five days before ovulation, and regression of large nonovulatory follicles a few days before Ovulation.

Journal ArticleDOI
TL;DR: Results suggest that the influence of stress on gonadotropin secretion, and subsequent reproductive responses, is dependent on the magnitude of the adrenal steroidogenic response and the animal's adaptation to stress, and indicate the necessity to minimize and monitor animal stress when studying LH secretion.

Journal ArticleDOI
TL;DR: These findings are the first reported that demonstrate that the zona-free bovine DE will develop during in vitro culture.

Journal ArticleDOI
TL;DR: Some morphological and endocrinological aspects of cystic follicles in cows, women, rats, and rabbits were reviewed to enhance understanding of the factors that cause them.

Journal ArticleDOI
TL;DR: A transrectal real-time ultrasound scanning of the cow reproductive tract allows the operator to view images of structures which normally can be only palpated as mentioned in this paper, which may be expected to aid both the researcher and the practicing veterinarian considerably in the future.

Journal ArticleDOI
TL;DR: CAP may be useful for storage of canine sperm at 5°C and SM may be satisfactory forstorage of equine sperm in a split-ejaculated design.

Journal ArticleDOI
TL;DR: Xylazine produced intrauterine pressure responses which did not differ statistically from those seen with oxytocin, and the pharmacological basis for the oxytocic-like action of xylazine is presently unknown.

Journal ArticleDOI
TL;DR: Preliminary results imply presence of H-Y in bovine embryos of the morula and blastocyst stages recovered at about 6–12 days of gestation, which allows selective implantation of male and female during embryo transfer.

Journal ArticleDOI
TL;DR: The addition of serum to uterine secretions was shown to opsonize Streptococcus zooepidemicus and significantly enhance bacterial phagocytosis by equine neutrophils and therefore demonstrated that the process was complement-dependent.


Journal ArticleDOI
TL;DR: The fertility of frozen-thawed and fresh semen from three stallions was compared in a trial using a randomized block design and 90 mares for 108 cycles and mean pregnancy rate with frozen semen was 86% of the rate attained with fresh semen.


Journal ArticleDOI
TL;DR: Culturing the embryos for 24 or 48 h or treating the embryos with pronase, trypsin, or antiserum after virus exposure and washing reduced the number of embryos carrying virus and lessened the amount of virus on each of the embryos.

Journal ArticleDOI
TL;DR: In postpartum mares, the number of location changes and the time spent in a horn were not different between the formerly gravid horn and the nongravid horn, and the reported frequent attachment of the vesicle to the formerly nong Gravid horn was not, therefore, attributable to a difference in the extent of mobility or the amount of time spend in the nongavernmental horn during the period of high intrauterine mobility.