Showing papers in "Theriogenology in 2010"

TL;DR: The correct approach to analyzing method agreement is discussed, whether the authors are considering agreement between two measurements on the same samples (repeatability) or two individuals using identical methodology on identical samples (reproducibility) or comparing two methods.

TL;DR: Ovulation and the age of the egg at sperm penetration is critical for conception, therefore, emphasis in research needs to be on the timing of insemination relative to ovulation, and thus on the detection of ovulation.

TL;DR: It is concluded that the optimal concentration of lecithin and egg yolk is 1% and 20%, respectively, along with 7% glycerol, and suggested that leCithin can be used as a substitute for egg yolks.

TL;DR: The net economic value of reproductive efficiency in dairy cattle was estimated using a stochastic dynamic simulation model and which reproductive factors have an important impact on economic efficiency were the involuntary culling cost and the return of milk production.

TL;DR: Oocyte yield from two levels of indicus-taurus breeds is compared and the efficiency of sexed sperm for in vitro embryo production is demonstrated and the potential for international movement of embryos is demonstrated.

TL;DR: In vitro fertilization of the oocytes from the cultured sheep PFs resulted in the embryos developing to the morula stage for the first time and IGF-I+GH was found to be the best in vitro development of the PFs.

TL;DR: The dominant association between retained fetal membranes and clinical endometritis was supported by an expert panel of clinicians and the risk factors appear to be associated with trauma of the female genital tract and disruption of the physical barriers to infection rather than fecal contamination.

TL;DR: Embryo loss between embryonic Days 7 and 16 in nonlactating cattle, Bos taurus, was analyzed using transfer of 2449 in vitro-produced IVP blastocysts, indicating faster embryonic development/trophoblast proliferation in heifers and length was lower in winter than in winter, whereas season did not affect embryo recoveries.

TL;DR: It is concluded that LBF reflected luteal function better than LS specifically during lutea regression, and values represented highly reliable predictors of lutenal status.

TL;DR: The comprehension of molecular mechanisms underlying sperm-oviduct interaction may advance the understanding of the behavior of sperm within the female reproductive tract and provide new tools for sperm selection, extension of fertile life and modulation of capacitation in the field of reproductive biotechnologies.

TL;DR: Examining the effects of storage conditions on viability and fertilization ability of fresh ram (Ovis aries) semen up to 72h postcollection found storage at 5 degrees C maintained acceptable motility and viability up to72h compared with that of storage at 15 degrees C.

TL;DR: Cooling of testis tissue for up to 72h maintained structural integrity, cell viability, in vivo growth, and developmental potential up to complete spermatogenesis comparable with that of fresh tissue (control).

TL;DR: It is becoming clear that the markers used to characterize the putative ESCs were inadequate, and future derivation and validation of equine embryonic or other pluripotent stem cells would benefit greatly from a reliable panel of molecular markers specific to pluripotency of the developing horse embryo.

TL;DR: Proteins within sperm and seminal plasma that could serve as biomarkers of semen quality and fertility in stallions are identified and may provide a good marker of fertility.

TL;DR: ECG at norgestomet implant removal increased the growth rate of the largest follicle (LF) from implant removal to TAI, the diameter of the LF at Tai, and rates of ovulation and pregnancy rates, and GnRH at TAI improved the synchrony of ovulations andregnancy rates in postpartum Nelore cows.

TL;DR: Although there are many reports on both the occurrence of female sperm storage and its adaptive benefits, few studies have been directed toward explaining the mechanisms involved and it is surprising that none have yet been elucidated by technologists wishing to improve the long-term storage of fresh semen.

TL;DR: This work has developed a simple and cost-efficient flow cytometric approach for identifying non-sperm particles that can be carried out in parallel with functional assessments and is suitable for obtaining accurate sperm counts during routine semen evaluation.

TL;DR: Some of the factors that make the establishment of ESC lines in the pig, and in animal species other than mouse and human, a very slow process are discussed.

TL;DR: Anthocyanin treatment during IVM improved developmental competence of SCNT embryos, most likely by increasing intracellular GSH synthesis, reducing ROS level, and stimulating nuclear reprogramming via increased transcription factor expression.

TL;DR: These results are the first to demonstrate that use of a combination of recombinant growth factors and cytokine, as IGF-I, IGF-II, bFGF, TGF-beta1, LIF, and GM-CSF, produces similar results to 10% fetal calf serum for the development of in vitro-produced bovine embryos.

TL;DR: This study strengthens the hypothesis of a prominent role of photoperiod in seasonal infertility and of an additional role of heat stress the hottest years.

TL;DR: Frozen non-sexed (NS) sperm had better quality than sexed sperm, including higher motility and greater percentages of cells with an intact membrane and acrosome, and embryo development kinetics was similar.

TL;DR: The freezing-thawing procedure significantly affects PS translocation, the intracellular H( 2)O(2) level, and DNA integrity in canine semen, which may explain the lower fertilization rate and in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) outcome when frozen-thawed spermatozoa are used.

TL;DR: It is proposed that the CASA evaluation of motility of bovine frozen-thawed semen using Hamilton-Thorne IVOS 12.3 should be performed using a frame rate of 60 frame/s and 30 frames per field, regardless of the chamber used.

TL;DR: In conclusion, repeat breeder dairy cows had very poor reproductive performance, and lower parity, abnormal resumption of postpartum ovarian cycles, and shorter days in milk at first AI were risk factors for repeat breeding.

TL;DR: The results showed that hyperlactatemia, although it does not provide diagnostic information, indicates the severity of illness and the need for an early and aggressive intervention in critically ill foals, and lactatemia proved to be a reliable prognostic parameter.

TL;DR: It is concluded that the mitochondrial pathway of apoptosis seems to be an important factor involved in the sublethal damage that equine spermatozoa experience after freezing and thawing, and that sperm motility in the equine species is largely dependent on mitochondrial ATP produced by oxidative phosphorylation.

TL;DR: A clinical approach is discussed to determine the proportion of false positive findings that might occur by vaginal inspection in cows diagnosed with clinical endometritis with findings obtained by vaginoscopy.

TL;DR: In conclusion, abnormal calving, retained placenta, and postpartum uterine infections may be associated with an increase in prevalence of endometritis and subsequently, SE may decrease reproductive performance and increase the incidence of repeat breeder syndrome.

TL;DR: In this paper, a computer-assisted sperm analyzer (CASA) system versus subjective microscropic examination as a means of assessing sperm motility was compared for streaked prochilod sperm.