Showing papers in "Virology in 1968"

TL;DR: Polyacrylamide gel electrophoresis revealed at least 25 proteins synthesized at one time or another during productive infection of HEp-2 cells with herpes simplex virus made in the cytoplasm.

TL;DR: This virus isolated from seawater is shown to contain lipids by its sensitivity to ethyl ether and by chemical analysis of the purified virus, showing the presence of at least two proteins, probably lipoproteins since they can be stained with Black Sudan.

TL;DR: Results imply that unique membranes of vaccinia can condense de novo from precursors, two of which are lecithin and viral protein(s), to become the envelope surrounding immature particles, and further differentiation into mature virus occurs inside this envelope.

TL;DR: The adenovirion has been shown to have a much more complex structure than was previously assumed, and eight different types of polypeptides have been assigned a place in the particle.

TL;DR: The fact that the poly G-binding, deoxycytidylate (dC)-rich clusters are restricted to the in vivo transcribing H strand, together with the absence of thymine-rich clusters in T7 DNA, is compatible with the hypothesis that pyrimidine- rich clusters are related to RNA transcription, possibly as the initiation and termination sites.

TL;DR: Herpes simplex virus DNA was isolated in an intact form by treating the virus particles with detergents and determined an average molecular weight of 100 × 106 daltons by electron microscopy and zone centrifugation in sucrose gradients.

TL;DR: Proteins made by E. coli cells infected with bacteriophage T4 were analyzed by a method which combined disc electrophoresis and autoradiography, showing that the early proteins are not controlled as a single homogeneous class all members of which are synthesized during the same time periods.

TL;DR: Conditional lethal mutants that fall into 18 different cistrons have been defined, and the relative order of some of them has been established.

TL;DR: Either cowpea chlorotic mottle virus- RNA or brome mosaic virus-RNA, coated with the protein of any one of the three viruses, was resistant to snake venom phosphodiesterase and the progeny of hybrid viruses was that of their RNA.

TL;DR: The hydrodynamic behavior of cowpea chlorotic mottle virus suggests that it exists as two forms, depending on pH, but once the virus has been converted to the slowly sedimenting or swollen form, infectivity is lost because the nucleic acid is ruptured during swelling.

TL;DR: It is suggested that genes 4, 26, 28, 31, 38, 50, 51, and 65 are the most likely to code for gene products which function catalytically (if, in fact, any such functions exist).

TL;DR: Certain genetic sequences were proved by three-factor crosses involving a mutant adapted to guanidine resistance showing that recombination frequencies were additive, and an additive linear genetic map comprising one linkage group was obtained.

TL;DR: Primary yields from certain mixed infections carried out with ts mutants at restrictive temperatures show enhanced levels of parent type ts virus that in some cases represent 500-fold increases above the combined yields of ts virus obtained in single-infection controls.

TL;DR: The structure of bullet-shaped and truncated particles of vesicular stomatitis virus was examined by electron microscopy using negative staining and thin sectioning techniques and the conformation of the internal, probably nucleoprotein, component in intact and disintegrating particles was investigated.

TL;DR: Successive undiluted passages of SV40 (small plaque) in African green monkey kidney cells result in the production of heterogeneous defective particles of lower densities than that of infectious virions, which would account for defectiveness as well as alteration in density of virions.

TL;DR: It is concluded that the “defectiveness” of the Bryan strain of RSV is not based on a lack of the group-specific antigen in the virus particle.

TL;DR: Factors other than interferon may be responsible for part of the antiviral protection observed with the carboxylate copolymer, which induced prolonged protection against lethal Mengo virus infection in the mouse.

TL;DR: Results indicate that guanidine does not inhibit growth and completion of viral RNA chains, but blocks the initiation of new chains in cells that are actively producing virus.

TL;DR: A class of cytocidal mutants, designated cyt mutants, have been isolated from two highly tumorigenic strains of adenovirus type 12 (Ad. 12), i.e., Huie and 1131 as mentioned in this paper.

TL;DR: Five temperature-sensitive clear-plaque mutants of bacteriophage P2 were isolated, and the properties of strains lysogenic for these mutants were studied, finding that λ prophages, following induction by either temperature shift or ultraviolet irradiation, detach even in the absence of superinfection.

TL;DR: Genetic mapping and complementation tests have demonstrated the existence of two new cistrons, in addition to those previously described by Campbell, that are involved in particle morphogenesis in λ bacteriophage.

TL;DR: A mutant of phage T4 has been found which permits lysis of infected bacteria in the absence of lysozyme, and the new mutation may affect a phage-directed synthesis of bacterial cell wall components, hence the name spackle.

TL;DR: The purified virion preparation obtained by the third undiluted passage was shown to consist mainly of physically heterogeneous defective particles, the average buoyant density of defective virions was significantly lower than that of plaque-forming particles.

TL;DR: It was found that there were two classes of L-R lines, one producing RSV infectious for Japanese quail embryo or C A type chick embryo cells and another producing virus particles that were not detectably infectious for any cell line tested.

TL;DR: Antigenic mutants of the swine and BEL strains of influenza A virus were isolated after serial passage of these viruses in the presence of sublimiting concentrations of homologous antibody of low avidity, suggesting that antigenic variation among influenza viruses is related to changes in the amino acid sequence of their antigenic proteins.

TL;DR: Adenoviruses 12 and 31 were efficient in the induction of DNA synthesis and the patterns ofDNA synthesis induced by these viruses were early and of short duration, whereas adenovIRuses 3, 7, 2, and 5 were less efficient inThe patterns of DNA synthesisation induced by adenviruses 3 and 7 were late and long-lasting.

TL;DR: A collection of λbio transducing particles were isolated and the phage and bacterial extent of their genomes characterized, and the nature of the λdb particles conforms to the structural predictions of the Campbell model.