Showing papers in "Virology in 1979"

TL;DR: Xanthi-nc becomes resistant to tobacco mosaic virus (TMV) and produces b proteins when injected with the interferon inducer polyacrylic acid and injection of acetylsalicylic acid (aspirin) induces resistance and the formation of new proteins in the White Burley and Samsun NN cultivars of tobacco.

TL;DR: n-Butyrate was found to increase the number of virus producer cells to a dramatic extent in the Epstein-Barr virus-carrying P3HR-1 and B95-8 lines, raising interesting questions on the mechanism of EBV induction, and its possible relationship to the known differentiation inducing ability of n-butyrate.

TL;DR: Hybrid cell lines were prepared by the fusion of mouse myeloma cells with the spleen cells of C57BL/6 mice that were immunized with the AKR leukemia K36.

TL;DR: The results obtained permit the conclusion that in naturally occurring avian influenza viruses the structure of the hemagglutinin, that is its susceptibility to proteolytic cleavage in a broad spectrum of host cells, is the determining factor for pathogenicity.

TL;DR: It is concluded that the p21 of Ha-MuSV and Ki- MuSV are homologous proteins coded for bv homologueous sequences found in the recombinant genomes of Ha,MuSv and Ki,muSV.

TL;DR: The replication of Autographa californica nuclear polyhedrosis virus in Spodoptera frugiperda cells in culture has been studied with different methods and the pattern of viral protein synthesis was rather complex; within the first 24 hr postinfection, some 30-35 different polypeptides appeared sequentially in infected cells.

TL;DR: Changes in the electrophoretic pattern of newly synthesized products occur so as to decrease the number of polypeptides produced and to increase the yield of products which correspond to those seen in poliovirus-infected cells.

TL;DR: Three monoclonal hybridoma antibodies were used to select a total of 10 antigenic variants of A/Mem/1/71 (H3N2) virus and a dramatic change in antigenicity appeared to be associated with a single change in the amino acid sequence of the large hemagglutinin polypeptide, HA1.

TL;DR: The kinetics of replication of Autographa californica nuclear polyhedrosis virus (AcNPV) DNA in Spodoptera frugiperda cells in culture were studied and a certain heterogeneity of the AcNPV DNA was revealed.

TL;DR: The results have confirmed the role of the F protein in membrane fusion and have shown that the requirements for F protein activity are the previously demonstrated proteolytic processing of F, the insertion of theF protein into lipid, and the presence of an attachment mechanism.

TL;DR: These DNA homology groupings are consistent in the main with the properties of other “groupings” of human Ads, e.g., oncogenic groups (tumorigenicity in newborn hamsters), T-antigen groups, G + C content of viral DNA, hemagglutination groups, molecular characteristics of subviral particles and virion proteins, and human epidemiology and pathogenicity.

TL;DR: The results of these experiments demonstrate that the SV40 A gene product is required to initiate and possibly maintain the high levels of the 54,000 MW protein found in virus-infected and -transformed cells.

TL;DR: Viral DNA sequences present in the human embryo kidney cell line (293) transformed from sheared adenovirus 5 fragments reveals that the viral DNA is integrated and located at two sites on the cellular genome.

TL;DR: Infection of the trigeminal ganglion was investigated using standard thymidine kinase-positive (TK + ) herpes simplex virus (HSV) and two TK − mutants and results obtained were interpreted as being consistent with impaired axonal transport of Tk − HSV from cornea to trigeminals ganglions neurons or more likely, with impaired replication of TK + HSV in ganglionic neurons.

TL;DR: The results suggest that the 48K–55K species may originate either as host-coded species (perhaps induced by the virus) that share determinants with T Ag or perhaps as SV40-encoded species sharing only very little of the amino acid sequence of 94K T Ag.

TL;DR: Clones of rat cells transformed by avian myelocytomatosis virus strain MC29 and avian erythroblastosis virus have been isolated and display an altered morphology and are able to form colonies in soft agar.

TL;DR: The first virus-specific proteins in bluetongue virus (BTV)-infected cells can be demonstrated 2 to 4 hr after infection at 31° by immune precipitation and pulse-chase experiments indicate that the BTV polypeptides do not accumulate in the soluble fraction in the same ratio in which they are synthesized.

TL;DR: It is suggested that cleavage of the B RNA product, and of all other viral precursors which undergo rapid “primary” cleavage, involves an intramolecular reaction.

TL;DR: Nonproducer clones of chicken fibroblasts or erythroblasts transformed by avian eryth-roblastosis virus of strains R and ES4 were isolated and a 75,000 molecular weight protein (AEV-75K) was isolated, shown to be antigenically related to the gag gene, but not to the pol or env genes.

TL;DR: Virus specificity of the tubules was demonstrated by specific immune precipitation with antisera against purified virus and there is immunological cross-reaction between BTV and EHDV tubules.

TL;DR: The Vero host cell poly A+ RNA was found to contain both types I and II caps as well as internal Ns-methyladenine, and possible implications of the differences between dengue virus methylation and that of the host RNA and other mRNAs are discussed.

TL;DR: Arildone inhibits uncoating of poliovirus and thereby prevents virus-induced shutoff of host cell protein synthesis, and possible mechanisms by which arildone interacts with the poliov virus icosahedral capsid to prevent uncoatering are discussed.

TL;DR: In this paper, hybrid cells that produced monoclonal antibodies against the envelope proteins of murine leukemia virus (MuLV) were prepared by the polyethylene glycol-mediated fusion of a mouse myeloma cell line with lymphocytes from mice immunized with allogeneic MuLV-producing leukemia cells.

TL;DR: The selective inhibition of host cell protein synthesis, both by Sindbis virus and by altered NaCl media, appears to result from changes in the intracellular na + and K + concentrations.

TL;DR: The results of the investigation of the synthesis of measles virus polypeptides in cells in their fifth passage after removal from the brain of a patient with subacute sclerosing panencephalitis support the previous suggestion that there is decreased synthesis of the M polypeptic in the cells of the brain in patients with SSPE.

TL;DR: The “dideoxy” sequencing method has been used to obtain 121 nucleotides of sequence of cDNA transcribed from the 3′ end of the RNA segment which contains the coding information for the hemagglutinin of influenza virus A/RI/5−/57 (H2N2).

TL;DR: Cytoplasmic RNA, isolated at various times after vaccinia virus infection, was translated in a message-dependent cell-free system prepared from rabbit reticulocytes, and a correlation between RNA sedimentation and molecular weight of translation product was obtained.

TL;DR: The AAV autointerference is correlated with production of the aberrant deleted AAV genomes and may be analogous to the previously characterized aberrant DNA molecules found in light-density AAV particles.

TL;DR: Chick embryo fibroblasts infected with vaccinia WR or cowpox virus were studied by immunofluorescence and electron microscopy to indicate an intimate association of mature progeny virus with the actin-containing microfilaments of the cellular cytoskeleton.

TL;DR: Treatment of primary cultures of chick embryo cells with homologous interferon increased the activity of 2′5′-oligoadenylate synthetase in their cytoplasmic extracts, suggesting that the enzyme and the polypeptide are each the product of a gene whose function is controlled byInterferon treatment.