Showing papers in "Virology in 1989"

TL;DR: The transposable IFP2 element of Trichoplusia ni was originally isolated as a host DNA insertion in spontaneous FP mutants of Galleria mellonella or Autographa californica nuclear polyhedrosis viruses, but is not apparent in DNAs isolated from the TN-R2 cell line or the authors' laboratory colony of T. ni larvae, suggesting IFP1 was recently introduced into the T. Ni genome.

TL;DR: High levels of nonfused chloramphenicol acetyltransferase, beta-galactosidase, and beta-glucuronidase expressed under the control of new vector constructs of the polyhedrin promoter in Spodoptera frugiperda cells infected with Autographa californica nuclear polyhedrosis virus were investigated by SDS-PAGE and RNA dot blot analysis of total cytoplasmic RNA.

TL;DR: A cDNA clone derived from genomic RNA of hog cholera virus was identified using an oligonucleotide complementary to the RNA encoding a hexapeptide from the putative RNA-dependent RNA polymerase of the closely related bovine viral diarrhea virus.

TL;DR: It is proposed through a sequence and structural-pattern analysis that a protein domain of undefined function encoded by the enveloped RNA flavi- and pestivruses is a Ser active-center enzyme related to the cellular trypsin family.

TL;DR: While the H1 and H3 hemagglutinins appear to be subject to similar host-derived selective pressures, there appears to be certain fundamental differences in the detailed molecular interaction of the two hemag GLUTinins with their sialyloligosaccharide receptor determinants.

TL;DR: A molecular clone of Venezuelan equine encephalitis virus (VEE) was constructed from four cDNAs that were synthesized using the viral RNA genome as template.

TL;DR: No major changes in the conformation of the Sabin vaccine poliovirus type 3 5'-UTR due to the transition in position 472 were observed, and the biological relevance of the conserved primary and secondary structure elements in the picornaviral5'-UTRs is discussed.

TL;DR: Results suggest that both the structural (E) and nonstructural NS1′ and NSV) glycoproteins were accumulated within the secretory pathway of the infected Vero cells, assembled into particles, and then released into the extracellular fluid.

TL;DR: It is indicated that ribonucleotide reductase is critical for productive acute and reactivatable latent infections in mice and replication in mouse cells at 38 degrees and cautioned that caution be exercised in extrapolating from studies conducted in mice to human infections when judging the utility of this enzyme as a target for antiviral chemotherapy.

TL;DR: It is demonstrated here that the dimerization of NS1 is associated with a change in hydrophobicity and sedimentability of this protein, which is consistent with NS1 becoming membrane-associated.

TL;DR: The gene encoding gp64, the envelope glycoprotein of the budded virus (BV) of Orgyia pseudotsugata multicapsid nuclear polyhedrosis virus (OpMNPV), was mapped to the HindIII-E fragment of of the viral genome and expression of the gp64 gene was examined at various times postinfection.

TL;DR: This work developed message-specific subgenomic clones of HPV-6 and HPV-11 in pGEM vectors based on the mRNA structures and revealed the qualitative and quantitative transcription patterns of different viral messages in relationship to one another, to viral DNA replication, and to cellular differentiation.

TL;DR: A model is presented which proposes different roles for conserved and variable positions in the TEV heptapeptide cleavage sequence.

TL;DR: It appears that the presence of a positively charged amino acid (arginine or lysine) in position 333 of the glycoprotein is necessary for viral virulence.

TL;DR: Both episomal and integrated forms of a complete HPV 16 DNA are involved in invasive cervical cancers, indicating that a full viral genome was present in these specimens.

TL;DR: The etiologic agent of psittacine beak and feather disease represents a previously undescribed viral pathogen, and a preliminary transmission study using purified virus induced pathological lesions characteristic of those observed in the natural disease.

TL;DR: The present study strongly supports the importance of the previously identified signals for gene expression and for the proteolytic activation of the gene product and identifies three distinct evolutionary lineages correlating with the virulence as expressed by mean death time (MDT) for chick embryo.

TL;DR: An AAV mutant in which the putative AUG initiation codon in the p19 transcripts was altered and which did not express p19-coded rep proteins is described, which defines a novel phenotype for a rep gene mutation and suggests a role for the rep proteins in the generation or accumulation of the viral SS DNA.

TL;DR: The completed sequence of LCMV reveals a formerly unknown gene (Z) on the L genomic segment, which is encoded in the positive or message-sense of the viral genomic RNA, whereas the adjacent gene (L) is in the genome-complementary, or negative sense.

TL;DR: The present study of four persistent MVs revealed a high number of differences from a consensus sequence also in other genes, and classified four lytic and seven persistent MV strains on the basis of their sequences.

TL;DR: Nucleotide sequence analysis of cDNA clones covering the entire genomes of Trinidad donkey (TRD) Venezuelan equine encephalitis (VEE) virus and its vaccine derivative, TC-83, has revealed 11 differences.

TL;DR: An open reading frame from the chloramphenicol acetyltransferase (CAT) gene was fused behind a tobacco mosaic virus (TMV) subgenomic RNA promoter and inserted into different positions in the complete TMV genome to examine how much this viral genome can be altered with continued replication as mentioned in this paper.

TL;DR: It is concluded that VP5 also plays some part in serotype determination of these virus isolates, as analyzed by serum-neutralization, but its role may be less significant than that of VP2.

TL;DR: The subunit composition of env glycoproteins isolated from HIV-infected cell membranes, virus particles, and baculovirus recombinant- Infected insect cells has been investigated using a number of crosslinking reagents to provide evidence that gp160 is a tetramer of identical subunits and that gp41 is also tetrameric.

TL;DR: An evolutionary tree based on protein NS1 is presented that reveals the molecular basis of relationships among flaviviruses and shows how tick-borne and mosquito-borne flavivirus sequences share a common hydrophilicity profile.

TL;DR: A panel of monoclonal antibodies (MAbs) was prepared to analyze the antigenic structure of the tick-borne encephalitis (TBE) virus glycoprotein E, providing evidence of the role of the carbohydrate side chain in stabilizing these epitopes.

TL;DR: The proteolytic processes involved in the synthesis of the structural proteins of the West Nile (WN) flavivirus were analyzed and it is indicated that a virus-coded protease which can cleave the polyprotein after two basic amino acid residues is responsible for this cleavage.

TL;DR: A structural model of the TEV 49-kDa proteinase proposes other virus-specific differences in the vicinity of the active site triad and substrate-binding pocket that may explain the observed negligible effect of most cellular proteinase inhibitors on the activity of this viral proteinase.

TL;DR: Analysis of hemagglutinin-neuraminidase gene sequence among 13 strains of Newcastle disease virus isolated over the last 50 years demonstrated the existence of at least three distinct lineages, which must have co-circulated for considerable periods.

TL;DR: It is concluded that the coat protein of BCTV is essential for spread of the virus.