2,3-butanediol production by acetogenic bacteria, an alternative route to chemical synthesis, using industrial waste gas.
Michael Köpke,Christophe Daniel Mihalcea,Fungmin Liew,Joseph Henry Tizard,Mohammed S. Ali,Joshua Jeremy Conolly,Bakir Al-Sinawi,Sean Dennis Simpson +7 more
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TLDR
Evidence of 23BD production by three nonpathogenic acetogenic Clostridium species—Clostridia autoethanogenum, C. ljungdahlii, and C. ragsdalei—using carbon monoxide-containing industrial waste gases or syngas as the sole source of carbon and energy is presented.Abstract:
2,3-Butanediol (23BD) is a high-value chemical usually produced petrochemically but which can also be synthesized by some bacteria. To date, the best microbial 23BD production rates have been observed using pathogenic bacteria in fermentation systems that depend on sugars as the carbon and energy sources for product synthesis. Here we present evidence of 23BD production by three nonpathogenic acetogenic Clostridium species—Clostridium autoethanogenum, C. ljungdahlii, and C. ragsdalei—using carbon monoxide-containing industrial waste gases or syngas as the sole source of carbon and energy. Through an analysis of the C. ljungdahlii genome, the complete pathway from carbon monoxide to 23BD has been proposed. Homologues of the genes involved in this pathway were also confirmed for the other two species investigated. A gene expression study demonstrates a correlation between mRNA accumulation from 23BD biosynthetic genes and the onset of 23BD production, while a broader expression study of Wood-Ljungdahl pathway genes provides a transcription-level view of one of the oldest existing biochemical pathways.read more
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Clostridia: the importance of their exceptional substrate and metabolite diversity for biofuel and biorefinery applications.
TL;DR: Pathway engineering to combine established substrate-utilization programs, such as for cellulose, CO2/H2 or CO, with desirable metabolic programs could lead to modular design of strains suitable for many applications.
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Review of old chemistry and new catalytic advances in the on-purpose synthesis of butadiene
TL;DR: A comprehensive summary of the current state of knowledge regarding advances and achievements in the field of the chemocatalytic conversion of ethanol and butanediols to butadiene is presented, including thermodynamics and kinetic aspects of the reactions with discussions on the reaction pathways and the type of catalysts developed.
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Commercial Biomass Syngas Fermentation
TL;DR: The use of gas fermentation for the production of low carbon biofuels such as ethanol or butanol from lignocellulosic biomass is an area currently undergoing intensive research and development, with the first commercial units expected to commence operation in the near future as discussed by the authors.
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Gas Fermentation—A Flexible Platform for Commercial Scale Production of Low-Carbon-Fuels and Chemicals from Waste and Renewable Feedstocks
Fungmin Liew,Michael Martin,Ryan Tappel,Bjorn Daniel Heijstra,Christophe Daniel Mihalcea,Michael Köpke +5 more
TL;DR: This review gives an overview of the gas fermentation process, focusing specifically on anaerobic acetogens, and applications of synthetic biology and coupling gas fermentation to additional processes are discussed in detail.
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Electrobiocommodities: powering microbial production of fuels and commodity chemicals from carbon dioxide with electricity
Derek R. Lovley,Kelly P. Nevin +1 more
TL;DR: Substantial research and development will be required for E-BC commercialization, and acetogenic microorganisms are the only organisms shown to covert electrically generated low potential electrons and carbon dioxide into multi-carbon organic products with high recovery of electrons in product.
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