2.45-GHz microwave irradiation adversely affects reproductive function in male mouse, Mus musculus by inducing oxidative and nitrosative stress
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Cites methods from "2.45-GHz microwave irradiation adve..."
...In the first step, after initial deparafinization in xylene and rehydration in graded series of alcohol, slides were incubated with goat- full length p53 antisera (dilution 1:100), rabbit- Bax antisera (dilution 1:100), rabbit- proCaspase-3 antisera (dilution 1:100) and mouse- full length/uncleaved PARP-1 antisera (dilution 1:100) for 24 h in humid chamber respectively (Shahin et al., 2014)....
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...The SAR value was estimated for body length parallel to the electric field, as per actual placement of the mouse (Gandhi et al., 1977; Shahin et al., 2013, 2014)....
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...…alcohol, slides were incubated with goat- full length p53 antisera (dilution 1:100), rabbit- Bax antisera (dilution 1:100), rabbit- proCaspase-3 antisera (dilution 1:100) and mouse- full length/uncleaved PARP-1 antisera (dilution 1:100) for 24 h in humid chamber respectively (Shahin et al., 2014)....
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34 citations
Cites background from "2.45-GHz microwave irradiation adve..."
...Shahin et al. (2014) exposed rats to the nonthermal low-level 2.45 GHz EMR (CW for 2 h day21 during 30 days, power density 0.029812 mW cm22 and SAR 0.018 W kg21), and they observed that EMR irradiation induced a significant decrease in a sperm count and sperm viability, along with the decrease in a…...
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...Shahin et al. (2014) applied 2.45-GHz microwave irradiation for 2 h day21 during 30 days and found a decrease in testosterone levels in the exposed group of mice....
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33 citations
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...2000 [27] with slight modification [28]....
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33 citations
Cites background or methods from "2.45-GHz microwave irradiation adve..."
...The SAR value was estimated for body length parallel to the electric field, as per actual placement of the mouse (Gandhi et al., 1977; Shahin et al., 2013, 2014)....
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...…and other laboratories have demonstrated MW radiation as one of the strongest environmental stressor, responsible for inducing oxidative/nitrosative stress by havoc generation of free radicals (reactive oxygen [ROS]/nitrogen species [RNS]) (Shahin et al., 2013, 2014, 2015; Yakymenko et al., 2016)....
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References
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...After electrophoresis, the gel was subjected to substrate-specific staining of SOD bands as described by Beauchamp and Fridovich [46]....
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...Free radicals are also implicated in the induction of apoptosis [23] and cellular senescence [24]....
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