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Journal ArticleDOI

2-Methoxy-3,4-Dihydroxybenzoic Acid and other Compounds from Ramaria aurea AND Clavariadelphus ligula

13 May 2014-Chemistry of Natural Compounds (Springer US)-Vol. 50, Iss: 2, pp 391-393
TL;DR: The goal of the present work was to study the chemistry of R. aurea and C. ligula fruiting bodies and found that extracts of these fungi possessed cytotoxic and antiradical activity.
Abstract: Ramaria aurea (Schaeff.) Quel. and Clavariadelphus ligula (Schaeff.) Donk. are fungal species of the family Gomphaceae and representatives of the class Agaricomycetes that are widely distributed over Siberia and the Russian Far East. Young fruiting bodies of both species are used as food because of the high gustatory qualities. Information on the chemical components and biological activity of R. aurea and C. ligula has not been published. Preliminary investigations found that extracts of these fungi possessed cytotoxic and antiradical activity. The goal of the present work was to study the chemistry of R. aurea and C. ligula fruiting bodies. Fruiting bodies of these plants were collected in the vicinity of Pykhta Gap (Zaigraev District, Republic of Buryatiya,
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Journal ArticleDOI
TL;DR: Considering that the activity of both the UPP and ALP pathways decreases with aging, the results suggest that the hydroxybenzoic acid scaffold could be considered as a promising candidate for the development of novel modulators of the proteostasis network, and likely of anti-aging agents.
Abstract: A main cellular functional module that becomes dysfunctional during aging is the proteostasis network. In the present study, we show that benzoic acid derivatives isolated from Bjerkandera adusta promote the activity of the two main protein degradation systems, namely the ubiquitin-proteasome (UPP) and especially the autophagy-lysosome pathway (ALP) in human foreskin fibroblasts. Our findings were further supported by in silico studies, where all compounds were found to be putative binders of both cathepsins B and L. Among them, compound 3 (3-chloro-4-methoxybenzoic acid) showed the most potent interaction with both enzymes, which justifies the strong activation of cathepsins B and L (467.3 ± 3.9%) on cell-based assays. Considering that the activity of both the UPP and ALP pathways decreases with aging, our results suggest that the hydroxybenzoic acid scaffold could be considered as a promising candidate for the development of novel modulators of the proteostasis network, and likely of anti-aging agents.

8 citations

References
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Journal ArticleDOI
TL;DR: The underlying molecular phenomena of the RSA of flavonoids could be explained by the ease of hydrogen atom abstraction and the ease-of- termination of the flavonoidal aroxyl radicals.

584 citations

Journal ArticleDOI
TL;DR: The effects of boiling and steaming processes on the phenolic components and antioxidant activities of whole yellow and black soybeans were investigated and quantitatively determined by HPLC.
Abstract: The effects of boiling and steaming processes on the phenolic components and antioxidant activities of whole yellow (with yellow seed coat and yellow cotyledon) and black (with black seed coat and green cotyledon) soybeans were investigated. As compared to the raw soybeans, all processing methods caused significant (p < 0.05) decreases in total phenolic content (TPC), total flavonoid content (TFC), condensed tannin content (CTC), monomeric anthocyanin content (MAC), DPPH free radical scavenging activity (DPPH), ferric reducing antioxidant power (FRAP), and oxygen radical absorbing capacity (ORAC) in black soybeans. Pressure steaming caused significant (p < 0.05) increases in TPC, CTC, DPPH, FRAP, and ORAC in yellow soybeans. The steaming resulted in a greater retention of TPC, DPPH, FRAP, and ORAC values in both yellow and black soybeans as compared to the boiling treatments. To further investigate the effect of processing on phenolic compounds and elucidate the contribution of these compounds to changes of antioxidant activities, phenolic acids, isoflavones, and anthocyanins were quantitatively determined by HPLC. The pressure steaming treatments caused significant (p < 0.05) increases in gallic acid and 2,3,4-trihydroxybenzoic acid, whereas all treatments caused significant (p < 0.05) decreases in two predominant phenolic acids (chlorogenic acid and trans-cinnamic acid), and total phenolic acids for both yellow and black soybeans. All thermal processing caused significant (p < 0.05) increases in aglucones and beta-glucosides of isoflavones, but caused significant (p < 0.05) decreases in malonylglucosides of isoflavones for both yellow and black soybeans. All thermal processing caused significant (p < 0.05) decreases of cyanidin-3-glucoside and peonidin-3-glucoside in black soybeans. Significant correlations existed between selected phenolic compositions, isoflavone and anthocyanin contents, and antioxidant properties of cooked soybeans.

271 citations

Journal ArticleDOI
TL;DR: It is suggested that pomegranate fruit extract can be a useful chemopreventive/chemotherapeutic agent against human lung cancer.
Abstract: Developing novel mechanism-based chemopreventive approaches for lung cancer through the use of dietary substances which humans can accept has become an important goal. In the present study, employing normal human bronchial epithelial cells (NHBE) and human lung carcinoma A549 cells, we first compared the growth inhibitory effects of pomegranate fruit extract (PFE). Treatment of PFE (50-150 microg/ml) for 72 h was found to result in a decrease in the viability of A549 cells but had only minimal effects on NHBE cells as assessed by the MTT and Trypan blue assays. PFE treatment of A549 cells also resulted in dose-dependent arrest of cells in G0-G1 phase of the cell cycle (as assessed by DNA cell cycle analysis). We further found that PFE treatment also resulted in (i) induction of WAF1/p21 and KIP1/p27, (ii) decrease in the protein expressions of cyclins D1, D2 and E, and (iii) decrease in cyclin-dependent kinase (cdk) 2, cdk4 and cdk6 expression. The treatment of cells with PFE inhibited (i) phosphorylation of MAPK proteins, (ii) inhibition of PI3K, (iii) phosphorylation of Akt at Thr308, (iv) NF-kappaB and IKKalpha, (v) degradation and phosphorylation of IkappaBalpha, and (vi) Ki-67 and PCNA. We also found that PFE treatment to A549 cells resulted in inhibition of NF-kappaB DNA-binding activity. Oral administration of PFE (0.1 and 0.2%, wt/vol) to athymic nude mice implanted with A549 cells resulted in a significant inhibition in tumor growth. Our results provide a suggestion that PFE can be a useful chemopreventive/chemotherapeutic agent against human lung cancer.

144 citations

Journal ArticleDOI
TL;DR: In this paper, the structures of these new compounds were elucidated on the basis of their spectral data, and they were reported for the first time from a fungal source, Lentinus edodes, Flammulina velutipes, Hypsizigus marmoreus, Pleurotus ostreatus and Pholiota nameko together with fifteen known ones.
Abstract: Eight new sterols, 5α, 8α-epidioxy-(22E, 24R)-23-methylergosta-6, 22-dien-3β-ol (1), 3β, 5α, 9α-trihydroxy-(22E, 24R)-23-methylergosta-7, 22-dien-6-one (2), 3β, 5α, 9α-trihydroxy-(24S)-ergost-7-en-6-one (3), 3β, 5α, 9α, 14α-tetrahydroxy-(22E, 24R)-ergosta-7, 22-dien-6-one (4), (22E, 24R)-ergosta-7, 22-diene-3β, 5α, 6α, 9α-tetrol (5), 5α, 9α-epidioxy-3β-hydroxy-(22E, 24R)-ergosta-7, 22-dien-6-one (6), 5α, 9α-epidioxy-3β-hydroxy-(24S)-ergost-7-en-6-one (7) and 5α, 6α-epoxy-(22E, 24R)-ergosta-8, 22-diene-3β, 7β, 14α-triol (8), have been isolated from five edible mushrooms, Lentinus edodes, Flammulina velutipes, Hypsizigus marmoreus, Pleurotus ostreatus and Pholiota nameko together with fifteen known ones (9-23), of which two (16 and 17) are reported for the first time from a fungal source. The structures of these new compounds were elucidated on the basis of their spectral data.

100 citations

Journal ArticleDOI
TL;DR: It is suggested that EP can exert antitumor activity in multiple myeloma U266 cells partly with antiangiogenic activity targeting JAK2/STAT3 signaling pathway as a potent cancer preventive agent for treatment of multipleMyeloma cells.
Abstract: Ergosterol peroxide (EP) derived from edible mushroom has been shown to exert anti-tumor activity in several cancer cells. In the present study, anti-angiogenic activity of EP was investigated with the underlying molecular mechanisms in human multiple myeloma U266 cells. Despite weak cytotoxicity against U266 cells, EP suppressed phosphorylation, DNA binding activity and nuclear translocalization of signal transducer and activator of transcription 3 (STAT3) in U266 cells at nontoxic concentrations. Also, EP inhibited phosphorylation of the upstream kinases Janus kinase 2 (JAK2) and Src in a time-dependent manner. Furthermore, EP increased the expression of protein tyrosine phosphatase SHP-1 at protein and mRNA levels, and conversely silencing of the SHP-1 gene clearly blocked EP-mediated STAT3 inactivation. In addition, EP significantly decreased vascular endothelial growth factor (VEGF), one of STAT3 target genes at cellular and protein levels as well as disrupted in vitro tube formation assay. Moreover, EP significantly suppressed the growth of U266 cells inoculated in female BALB/c athymic nude mice and immunohistochemistry revealed that EP effectively reduced the expression of STAT3 and CD34 in tumor sections compared to untreated control. These findings suggest that EP can exert antitumor activity in multiple myeloma U266 cells partly with antiangiogenic activity targeting JAK2/STAT3 signaling pathway as a potent cancer preventive agent for treatment of multiple myeloma cells.

58 citations