Figure 3: 20-Hydroxyecdysone effects are mediated through Mas receptor activation. C2C12 mouse myoblasts were differentiated for 6 days into myotubes. (A) Mas antagonists (A1 and A779) blocked 20E and Ang 1-7 inhibitory activities on myostatin gene expression determined by qRT-PCR. (B) MAS siRNA abolished 20E- and Ang 1-7-induced myostatin inhibition. Results are shown as means ± SEM with *p < 0.05; **p < 0.01; ***p < 0.001. ANOVA with Dunnett’s test compared to untreated control and Mann-Whitney test was performed to compare two groups with # p<0.05; ## p<0.01, ### p<0.001.
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