3-Å resolution structure of a protein with histone-like properties in prokaryotes
TL;DR: The 3-Å structure of DNA-binding protein II, which exhibits histone-like properties in bacteria, has been determined and a mechanism by which the protein induces DNA supercoiling is proposed.
Abstract: The 3-A structure of DNA-binding protein II, which exhibits histone-like properties in bacteria, has been determined. The molecule is dimeric and appears to bind to the phosphate backbone of DNA through two symmetry-related arms. A mechanism by which the protein induces DNA supercoiling is proposed.
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TL;DR: A cDNA clone that codes for a new tissue-specific DNA binding protein, PU.1, was isolated and was shown to be a transcriptional activator that is expressed in macrophages and B cells.
923 citations
TL;DR: DNA bending induced by the H MG domain can facilitate the formation of higher-order nucleoprotein complexes, suggesting that HMG domain proteins may have an architectural role in assembling such complexes.
804 citations
TL;DR: The crystal structure of IHF complexed with 35 bp of DNA is reported, providing evidence for the importance of a narrow minor groove in IHF's binding sequence.
780 citations
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...To move the phosphate backbone out of the way of the neighboring same as that of HU (Tanaka et al., 1984; White et al., 1989): the bodies of the two proteins can be superim-complex, the bp 59 to the nick adopts a Hoogsteen configuration, and the one before that forms a reverse posed with a…...
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702 citations
TL;DR: Human CTF/NF-I consists of a family of CCAAT box binding proteins that activate both transcription and DNA replication and analysis of cDNA mutants reveals that the N-terminal portion of CTF-1 is sufficient for site-specific DNA recognition, protein dimerization, and adenovirus replication.
701 citations
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TL;DR: The molecular structures presented have the most probable values of bond-lengths, bond-angles and furanose ring conformations as defined by accurate X-ray crystallographic analyses of relevant monomers.
895 citations
TL;DR: The gradual bimodal decrease in sedimentation rate seen after amino-acid starvation suggests that the faster sedimenting structures in exponentially growing cells represent the folded chromosomes at more advanced stages in their replication cycle.
587 citations
TL;DR: The three-dimensional structure of the 66-amino acid cro repressor protein of bacteriophage λ suggests how it binds to its operator DNA and suggests a pair of 2-fold-related α-helices of the represser seem to be a major determinant in recognition and binding.
Abstract: The three-dimensional structure of the 66-amino acid cro repressor protein of bacteriophage λ suggests how it binds to its operator DNA. We propose that a dimer of cro protein is bound to the B-form of DNA with the 2-fold axis of the dimer coincident with the 2-fold axis of DNA. A pair of 2-fold-related α-helices of the represser, lying within successive major grooves of the DNA, seem to be a major determinant in recognition and binding. In addition, the C-terminal residues of the protein, some of which are disordered in the absence of DNA, appear to contribute to the binding.
486 citations
TL;DR: Model-building studies of the repressor–operator complex suggest that α-helices, especially the N-terminal parts of these helices, may provide a useful surface for protein–DNA interactions.
Abstract: The structure of the operator-binding domain of the λ repressor has been determined at 3.2 A resolution. This domain contains an extended N-terminal arm and five α-helices. Model-building studies of the repressor–operator complex suggest that α-helices, especially the N-terminal parts of these helices, may provide a useful surface for protein–DNA interactions.
462 citations
TL;DR: The incubation of the E coli DNA binding protein HU with relaxed circular SV40 DNA in the presence of pure nicking-closing enzyme introduces up to 18 negative superhelical turns in the DNA molecules as measured by agarose gel electrophoresis.
431 citations