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Journal ArticleDOI

3-nitrotyrosine And Soluble Vascular And Intracellular Adhesion Molecule Responses To High-intensity Interval And Steady-state Moderate-intensity Exercise: 626 Board #22 May 27, 2

01 May 2015-Medicine and Science in Sports and Exercise-Vol. 47, pp 158-159

TL;DR: Transient elevations in NT and sVCAM-1 after moderate-intensity SSE but not HIIE of similar average intensity and duration may indicate unique effects of interval exercise.

AbstractVascular endothelium may respond differently to high-intensity interval exercise (HIIE) when compared to moderate-intensity steady state exercise (SSE). We hypothesized that greater sympathetic stimulation of soluble vascular adhesion molecule-1 (sVCAM-1) and intracellular adhesion molecule-1 (sICAM-1) and greater oxidative and nitrative stress on the vascular endothelium may transiently result from HIIE. PURPOSE: Determine the influence of HIIE on sVCAM-1, sICAM-1 and 3-nitrotyrosine (NT), a marker of nitric oxide-dependent reactive nitrogen species and nitrative stress, relative to a comparable amount of moderate-intensity SSE and a dose that is half that of SSE. METHODS: Seventeen male participants (age 27.8 + 6.4 yr; weight 80.6 + 9.0 kg; BMI 25.1 + 2.4 kg/m2; %fat = 19 + 5; VO2max 52.1 + 7.5 ml/kg/min) underwent HIIE by treadmill running (90% and 40% of VO2reserve in 3:2 min ratio) to expend 500 kcals (H500); HIIE to expend 250 kcals (H250), and; SSE at 70% VO2reserve to expend 500 kcals (M500) in a randomized crossover design. Intensities of all exercise conditions averaged 70% VO2reserve. Blood measures of sVCAM-1 (ng/mL), sICAM-1 (ng/mL), NT (nM), epinephrine (EPI) and norepinephrine (NE) in pg/mL, were obtained just before, immediately after, 2 hr and 24 hr after exercise. Significant differences were determined using 3 by 4 repeated measures ANOVAs. Effect sizes were calculated to determine the magnitude of dependent variable responses to exercise. RESULTS: HIIE resulted in 2 to 2.5 fold greater EPI responses immediately after exercise versus SSE (p = 0.0059, H250 ES = 1.89; H500 ES = 3.04). NE increased an average of 5.4 times above pre-exercise values across all exercise conditions (p < 0.0001). NT decreased immediately after HIIE (H250 ES = 0.39; H500 ES = -0.97) and returned to baseline by 2 hr post-exercise; whereas, NT was elevated 111% 2 hr (ES = 2.46) and remained 24 hr after SSE (p = 0.0001). sVCAM-1 was unchanged with HIIE but increased 6% immediately following moderate-intensity SSE and remained elevated 24 hr post-exercise (p < 0.0005, ES = 1.01). SUMMARY: Our results are in direct opposition to our hypothesis. Transient elevations in NT and sVCAM-1 after moderate-intensity SSE but not HIIE of similar average intensity and duration may indicate unique effects of interval exercise. NT and sVCAM-1 were not elevated after HIIE in spite of a greater sympathetic response than what was observed after moderate-intensity SSE.

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Summary

  • Vascular endothelium may respond differently to high-intensity interval exercise (HIIE) when compared to moderate-intensity steady state exercise (SSE).
  • The authors hypothesized that greater sympathetic stimulation of soluble vascular adhesion molecule-1 (sVCAM-1) and intracellular adhesion molecule-1 (sICAM-1) and greater oxidative and nitrative stress on the vascular endothelium may transiently result from HIIE.
  • Determine the influence of HIIE on sVCAM-1, sICAM-1 and 3-nitrotyrosine (NT), a marker of nitric oxide-dependent reactive nitrogen species and nitrative stress, relative to a comparable amount of moderate-intensity SSE and a dose that is half that of SSE, also known as PURPOSE.
  • Intensities of all exercise conditions averaged 70% VO2reserve.
  • Blood measures of sVCAM-1 (ng/mL), sICAM-1 (ng/mL), NT (nM), epinephrine (EPI) and norepinephrine (NE) in pg/mL, were obtained just before, immediately after, 2 hr and 24 hr after exercise.
  • Significant differences were determined using 3 by 4 repeated measures ANOVAs.
  • Effect sizes were calculated to determine the magnitude of dependent variable responses to exercise.
  • NE increased an average of 5.4 times above pre-exercise values across all exercise conditions (p < 0.0001).
  • SVCAM-1 was unchanged with HIIE but increased 6% immediately following moderate-intensity SSE and remained elevated 24 hr post-exercise (p < 0.0005, ES = 1.01).
  • The authors results are in direct opposition to their hypothesis, also known as SUMMARY.
  • NT and sVCAM-1 were not elevated after HIIE in spite of a greater sympathetic response than what was observed after moderate-intensity SSE.

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International Journal of Exercise Science www.tacsm.org
TACSM Abstract
3-Nitrotyrosine and Soluble Vascular and Intracellular Adhesion
Molecule Responses to High-Intensity Interval and Steady-State
Moderate-Intensity Exercise
Forsse JS
1
, Papadakis Z
1,
Bane AA
1
, Taylor JK
2
, Qian L
2
, Marroquín FEM
1
, Grandjean
PW
1
1
Department of Health, Human Performance, and Recreation; Baylor University; Waco,
TX
2
Clinical Laboratory Science; Auburn University-Montgomery; Montgomery, AL
Category: Doctoral
Advisor / Mentor: Grandjean, Peter (Peter_Grandjean@baylor.edu)
ABSTRACT
Vascular endothelium may respond differently to high-intensity interval exercise (HIIE) when compared
to moderate-intensity steady state exercise (SSE). We hypothesized that greater sympathetic stimulation of
soluble vascular adhesion molecule-1 (sVCAM-1) and intracellular adhesion molecule-1 (sICAM-1) and
greater oxidative and nitrative stress on the vascular endothelium may transiently result from HIIE.
PURPOSE: Determine the influence of HIIE on sVCAM-1, sICAM-1 and 3-nitrotyrosine (NT), a marker of
nitric oxide-dependent reactive nitrogen species and nitrative stress, relative to a comparable amount of
moderate-intensity SSE and a dose that is half that of SSE. METHODS: Seventeen male participants (age
27.8 + 6.4 yr; weight 80.6 + 9.0 kg; BMI 25.1 + 2.4 kg/m2; %fat = 19 + 5; VO2max 52.1 + 7.5 ml/kg/min)
underwent HIIE by treadmill running (90% and 40% of VO2reserve in 3:2 min ratio) to expend 500 kcals
(H500); HIIE to expend 250 kcals (H250), and; SSE at 70% VO2reserve to expend 500 kcals (M500) in a
randomized crossover design. Intensities of all exercise conditions averaged 70% VO2reserve. Blood
measures of sVCAM-1 (ng/mL), sICAM-1 (ng/mL), NT (nM), epinephrine (EPI) and norepinephrine (NE)
in pg/mL, were obtained just before, immediately after, 2 hr and 24 hr after exercise. Significant
differences were determined using 3 by 4 repeated measures ANOVAs. Effect sizes were calculated to
determine the magnitude of dependent variable responses to exercise. RESULTS: HIIE resulted in 2 to 2.5
fold greater EPI responses immediately after exercise versus SSE (p = 0.0059, H250 ES = 1.89; H500 ES =
3.04). NE increased an average of 5.4 times above pre-exercise values across all exercise conditions (p <
0.0001). NT decreased immediately after HIIE (H250 ES = - 0.39; H500 ES = -0.97) and returned to baseline
by 2 hr post-exercise; whereas, NT was elevated 111% 2 hr (ES = 2.46) and remained 24 hr after SSE (p =
0.0001). sVCAM-1 was unchanged with HIIE but increased 6% immediately following moderate-intensity
SSE and remained elevated 24 hr post-exercise (p < 0.0005, ES = 1.01). SUMMARY: Our results are in
direct opposition to our hypothesis. Transient elevations in NT and sVCAM-1 after moderate-intensity SSE
but not HIIE of similar average intensity and duration may indicate unique effects of interval exercise. NT
and sVCAM-1 were not elevated after HIIE in spite of a greater sympathetic response than what was
observed after moderate-intensity SSE.
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Journal ArticleDOI
Abstract: People practicing high-intensity interval exercise (HIIE) fasted during the morning hours under a lack of sleep. Such a habit may jeopardize the health benefits related to HIIE and adequate sleep. Fifteen habitually good sleeper males (age 31.1 ± 5.3 SD year) completed on a treadmill two isocaloric (500 kcal) HIIE sessions (3:2 min work:rest) averaged at 70% VO2reserve after 9-9.5 h of reference sleep exercise (RSE) and after 3-3.5 h of acute-partial sleep deprivation exercise (SSE). Diet and sleep patterns were controlled both 1 week prior and 2 days leading up to RSE and SSE. HIIE related performance and substrate utilization data were obtained from the continuous analysis of respiratory gases. Data were analyzed using repeated measures ANOVA with the baseline maximum oxygen uptake (VO2max) and body fat percentage (BF%) as covariates at p < 0.05. No difference was observed in VO2max, time to complete the HIIE, VE, RER, CHO%, and FAT% utilization during the experimental conditions. Whether attaining an adequate amount of sleep or not, the fasted HIIE performance and metabolism were not affected. We propose to practice the fasted HIIE under adequate sleep to receive the pleiotropic beneficial effects of sleep to the human body.