3H-tetracycline as a proxy for 41Ca for measuring dietary perturbations of bone resorption
Summary (1 min read)
1. Introduction
- Osteoporosis, a disease that is characterized by low bone mass, warrants attention.
- Therefore, high dietary calcium reduces bone remodeling, which results in an immediate reduction in fracture risk before any changes in bone mass or bone balance are detected [6].
- Therefore, there is a need for a more sensitive but yet direct method of assessing bone status in response to treatments.
2. Experimental
- Sixty-four ovariectomized (OVX) 6-month-old rats were purchased from Harlan (Indianapolis, IN).
- Rats were randomized to receive either 0.2% calcium or 0.5% dietary calcium treatment.
- SAS statistical software (version 8.0, SAS Institute, Cary, NC) was used for all analyses.
- Constant variance and normality assumptions were improved when 3H-TC in bone and urine was transformed by taking the log of the original data.
3. Results and discussion
- The effect of calcium intake on calcium balance over time is shown in Fig.
- Calcium retention differed between rats fed high and low calcium intakes in the rats stabilized to OVX, but in the early OVX rats, effects of loss of estrogen on bone resorption outweighed effects due to dietary calcium.
- Others [14,15] have also demonstrated changes in urinary 3H-TC levels in an acute setting during dietary interventions that lasted for 10 days.
- The authors are unable to resolve whether the effects of dietary calcium on bone turnover are transcient or chronic.
Acknowledgments
- The authors thank Dr. Alan York (Purdue University, USA) for supplying Dermastid beetles, Ania Kempa-Steczko, Pamela Lachcik, Mary Larimore, and Doug Maish for their technical assistance.
- This project was partially supported by NIH project P50 AT00477-01 and the Purdue botanicals research center.
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Cites background from "3H-tetracycline as a proxy for 41Ca..."
...Previous research in rats (35) and humans (36) demonstrated positive effects of calcium supplementation on bone in late, but not early, menopause....
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References
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"3H-tetracycline as a proxy for 41Ca..." refers background or methods in this paper
...A further limitation is that in rodents, a relatively small proportion of dietary calcium is being excreted in the urine of rats (3–6% in rats versus 14% in humans) [4]....
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...0 mmol/day) was due to an increase in absorbed calcium and suppression of bone resorption without affecting bone formation [4]....
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...Total calcium in bone, urine, and feces was measured by atomic absorption spectrophotometry as previously described [4] (A Analyst 300, Perkin Elmer)....
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88 citations
87 citations
72 citations
"3H-tetracycline as a proxy for 41Ca..." refers background in this paper
...Therefore, high dietary calcium reduces bone remodeling, which results in an immediate reduction in fracture risk before any changes in bone mass or bone balance are detected [6]....
[...]
...There is emerging evidence that calcium functions as an indirect regulator of remodeling of the skeleton [6]....
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Frequently Asked Questions (2)
Q2. What are the future works in "3h-tetracycline as a proxy for 41ca for measuring dietary perturbations of bone resorption" ?
Further research is needed with increased power to evaluate chronic effects of diet on urinary 3H-TC.