3H-tetracycline as a proxy for 41Ca for measuring dietary perturbations of bone resorption
Summary (1 min read)
1. Introduction
- Osteoporosis, a disease that is characterized by low bone mass, warrants attention.
- Therefore, high dietary calcium reduces bone remodeling, which results in an immediate reduction in fracture risk before any changes in bone mass or bone balance are detected [6].
- Therefore, there is a need for a more sensitive but yet direct method of assessing bone status in response to treatments.
2. Experimental
- Sixty-four ovariectomized (OVX) 6-month-old rats were purchased from Harlan (Indianapolis, IN).
- Rats were randomized to receive either 0.2% calcium or 0.5% dietary calcium treatment.
- SAS statistical software (version 8.0, SAS Institute, Cary, NC) was used for all analyses.
- Constant variance and normality assumptions were improved when 3H-TC in bone and urine was transformed by taking the log of the original data.
3. Results and discussion
- The effect of calcium intake on calcium balance over time is shown in Fig.
- Calcium retention differed between rats fed high and low calcium intakes in the rats stabilized to OVX, but in the early OVX rats, effects of loss of estrogen on bone resorption outweighed effects due to dietary calcium.
- Others [14,15] have also demonstrated changes in urinary 3H-TC levels in an acute setting during dietary interventions that lasted for 10 days.
- The authors are unable to resolve whether the effects of dietary calcium on bone turnover are transcient or chronic.
Acknowledgments
- The authors thank Dr. Alan York (Purdue University, USA) for supplying Dermastid beetles, Ania Kempa-Steczko, Pamela Lachcik, Mary Larimore, and Doug Maish for their technical assistance.
- This project was partially supported by NIH project P50 AT00477-01 and the Purdue botanicals research center.
Did you find this useful? Give us your feedback
Citations
26 citations
24 citations
17 citations
Cites background from "3H-tetracycline as a proxy for 41Ca..."
...Previous research in rats (35) and humans (36) demonstrated positive effects of calcium supplementation on bone in late, but not early, menopause....
[...]
10 citations
10 citations
References
27 citations
"3H-tetracycline as a proxy for 41Ca..." refers background in this paper
...It has been reported that repeated exposure to the labeled material during early rapid skeletal growth period, such as in utero or shortly after birth, is required in order to study the normal behavior of calcium in adult rat skeleton [13,18]....
[...]
...2 g Ca/100 g dry weight in an acute setting [13]....
[...]
26 citations
15 citations
"3H-tetracycline as a proxy for 41Ca..." refers background in this paper
...It has been reported that repeated exposure to the labeled material during early rapid skeletal growth period, such as in utero or shortly after birth, is required in order to study the normal behavior of calcium in adult rat skeleton [13,18]....
[...]
9 citations
"3H-tetracycline as a proxy for 41Ca..." refers background or result in this paper
...It is also unlikely that a lack of diet effect on urinary (3)H-TC excretion beyond 1 month was due to the single (3)H-TC dosing regimen, because chronic pre-labeling [12] showed similar transient results....
[...]
...[12] demonstrated an increase in urinary (3)H-TC levels only during the first 3 weeks of a 9 week period of dietary calcium restriction (0....
[...]
...[12], because (3)H-TC could still be detected in the urine and skeleton up to 6 months post dose....
[...]
...[12] showed little differences in response to dietary calcium with age....
[...]
Related Papers (5)
Frequently Asked Questions (2)
Q2. What are the future works in "3h-tetracycline as a proxy for 41ca for measuring dietary perturbations of bone resorption" ?
Further research is needed with increased power to evaluate chronic effects of diet on urinary 3H-TC.