Figure 6. DMAMQ mediates its effects through stimulation of NADPH oxidase to increase intracellular ROS production. (a) Intracellular ROS increases in response to DMAMQ treatment in proliferating NSCs treated with DMAMQ in the low μM range, as measured by DCF fluorescence. (b) Co-treatment with DMAMQ and the NOX inhibitor, DPI, prevents the DMAMQ ROS burst, suggesting the ROS increase is associated with cellular signaling involving NADPH oxidase. One-way ANOVA, n = 4, *p < 0.05, **p < 0.01 (significantly different from untreated control).
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