A 3D model of quadrupole dielectrophoresis levitation
01 Oct 2016-pp 1-4
TL;DR: A 3D and 2D model of quadrupole levitation system of printed circuit board (PCB) based technology is presented and discussed and the proposed levitation DEP is used to trap and levitate microbeads.
Abstract: The Dielectrophoresis (DEP) is a phenomenon in which a force exists on a particle due to a non-uniform electric field. A DEP levitation is widely used technique for particle trapping and levitation, where it is used mainly with biological particles. In this work, a 3D and 2D model of quadrupole levitation system of printed circuit board (PCB) based technology is presented and discussed. The levitation DEP is an electro-kinetic technique resulting from the interaction of a non-uniform electric field and polarizable particles. The electric field and the interaction of a non-uniform electric field and polarizable particles were simulated using COMSOL Multiphysics®. The proposed levitation DEP is used to trap and levitate microbeads (Carboxyl Polystyrene particles). In the scope of paper, a levitation quadrupole electrodes based on PCB technology is designed to trap and levitate the microbeads to be viewed and fixed for measurement of dielectric properties by another techniques using COMSOL Multiphysics®.
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TL;DR: In this article, the authors presented an electro-kinetic platform that combines three DEP configurations based on printed circuit board (PCB) technology, which are: traveling, rotation and levitation DEP.
8 citations
Cites methods from "A 3D model of quadrupole dielectrop..."
...Consequently, the quadrupole design is preferred to generate the desired non-uniform electric field pattern [22]....
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TL;DR: The layout of electrokinetic DEP platform is developed using SSC, a scripting language which supports the automation of a specific layout design by commands, and the performance of the developed Electrokinetic platform using S SC versus the platforms based on the other traditional techniques is presented and evaluated.
2 citations
Cites background or methods from "A 3D model of quadrupole dielectrop..."
...While, quadrupole electrodes are the preferred design for levDEP force [3,14], as shown in Fig....
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...[14] Abdelbaset R, Ghallab YH, Abdelhamid H, Ismail Y, ElWakad MT....
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...(2) Defining of materials properties: where, copper is chosen for microelectrodes, FR-4 material is chosen for PCB substrate, deionized water is chosen for medium of particles, and microbeads is chosen for particles [13,14]....
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01 Dec 2019
TL;DR: A DEP based platform is proposed for manipulation and identification of the DNA fragments that are labeled on specific microbeads, however, this platform includes a set of electrodes that allows simultaneous manipulation, trapping and controlling.
Abstract: Deoxyribonucleic acid (DNA) is the inborn material in humans and almost all other organisms. The movement of neutral polarizable particles from the site of the lowest strength of the electric field to the site of the most strength is known as Dielectrophoresis (DEP). DEP Distinguished from their counterparts of other techniques in manipulation and identification of biological cells and DNA. Many applications of natural and artificial DNA forms depend on the studies dedicated to the characterization of DNA. It is able to differentiate between different types of cells by observing the response of it towards a non-uniform electric field. In this study, a DEP based platform is proposed for manipulation and identification of the DNA fragments that are labeled on specific microbead. However, this platform includes a set of electrodes that allows simultaneous manipulation, trapping and controlling. Different designs of DEP-based microelectrode in micro size are simulated using COMSOL Multiphysics 5.4. This comparison is studied in order to get the optimized design for trapping and identification of DNA fragments that are labeled on specific microbeads.
Cites background from "A 3D model of quadrupole dielectrop..."
...2) the size of the tested particles, and the dielectric properties of the particles compared to the surrounding medium [10]....
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...The substrate material of the platform is FR4-TG130 [10]....
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01 Dec 2019
TL;DR: A comparison between the three designs based on Dielectrophoresis phenomena is presented using the Finite element method (FEM) in order to achieve an efficient technique for cell's trapping and levitation.
Abstract: The trapping and the levitation of micro scale particles has been of interest to many scientists for many years. Therefore, more than one design based on Dielectrophoresis (DEP) phenomena has been extracted for this function. The most important of these designs are as follows: the cone plate design, the concentric rings planar design, and the quadrupole planar design. In this study, a comparison between the three designs are presented using the Finite element method (FEM) in order to achieve an efficient technique for cell's trapping and levitation. This comparison based on studying the direction, distribution, the density, and the strength of the electric field, in addition to the generated DEP force on particles. COMSOL MULTIPHYSICS is proposed for introducing the simulation for the three proposed designs.
Cites methods from "A 3D model of quadrupole dielectrop..."
...The proposed electric field is achieved by applying an electric potential to two opposite poles and counterpart with 180 ̊ phase shift to others [5,8]....
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References
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Book•
01 Jan 1978
974 citations
01 Oct 1989
TL;DR: In this article, an experimental observation of the electrostatic orientation and dielectrophoresis of DNA under a high-intensity field produced in a microfabricated electrode system is made, using the fluorescent method as a visualization technique.
Abstract: Experimental observation of the electrostatic orientation and dielectrophoresis of DNA under a high-intensity field produced in a microfabricated electrode system is made, using the fluorescent method as a visualization technique. It is shown that the orientation takes place under a field strength of 10/sup 6/ V/m and the frequency at around 1 MHz. The stretch and shrink of an individual molecule is observed as the field is switched on and off. The molecular size (i.e. number of base pairs) is obtainable by measuring the stretched length of the molecule. A special field configuration is designed for the efficient molecular size determination using this principle. Molecular manipulation techniques, the alignment and positioning of the individual molecule, and the cutting of DNA at arbitrary locations with an ultraviolet beam are developed. >
383 citations
"A 3D model of quadrupole dielectrop..." refers background in this paper
...The DEP force (direction and amplitude) depends on the electric field (frequency, amplitude and waveform), the size of particle and the dielectric properties of the particles compared to the surrounding medium [5-6]....
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TL;DR: In this review, a detailed analysis of the modeling of DEP‐based manipulation of the particles is provided, and the recent applications regarding the particle manipulation in microfluidic systems are presented.
Abstract: Dielectrophoresis (DEP) is the movement of a particle in a non-uniform electric field due to the interaction of the particle's dipole and spatial gradient of the electric field. DEP is a subtle solution to manipulate particles and cells at microscale due to its favorable scaling for the reduced size of the system. DEP has been utilized for many applications in microfluidic systems. In this review, a detailed analysis of the modeling of DEP-based manipulation of the particles is provided, and the recent applications regarding the particle manipulation in microfluidic systems (mainly the published works between 2007 and 2010) are presented.
324 citations
TL;DR: A system is described that combines the three complementary techniques of dielectrophoresis (DEP), travelling wave dielectric properties (TWD) and electrorotation (ROT) for the first time on a single, integrated chip (3 x 6 mm).
130 citations
"A 3D model of quadrupole dielectrop..." refers background in this paper
...Also the second configuration is complex to be implemented because of it need ground plate at near distance from ring planar and resistances between each two successive rings to permit applying gradient electric potential [8, 9]....
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TL;DR: Using a simple new physical technique, dielectrophoresis, living cells can rapidly be distinguished from dead ones, and collected separately, enabling remarkably selective collection of living cells from a mixture of living and dead cells.
Abstract: Using a simple new physical technique, dielectrophoresis, living cells can rapidly be distinguished from dead ones, and collected separately. A detailed study using yeast cells is reported here. A comparative study of the dielectrophoresis of aqueous suspensions of living and dead yeast (Saccharomyces cerevisiae) was made over a range of frequencies, aqueous conductance, field strength, and time. The amount collected in a given period was observed to increase with field strength and aqueous resistivity except in the highest ranges where a reverse trend occurs. At high field strengths reversal of collection occurs (i.e., dispersion). The frequency responses of living and dead cells are different, enabling remarkably selective collection of living cells from a mixture of living and dead cells. Cells collected in this manner survive.
101 citations