Fig. 2. Profile of phage adsorption to cells at different physiological states. Studies of bacteriophage PVP-SE1 adsorption to Salmonella Enteritidis in phosphate buffer at room temperature. (A) Adsorption profile of phage to viable, VBNC and dead cells (upper curves) and phage growth curve in viable cells (bottom curve). For phage growth, average values result from triplicates of one single assay and standard deviations are all below 20%. (B) Picture of plaque forming units (PFU) on a culture plate for phage adsorption counts. (C) Zoom-in over PFU on the bacterial lawn. Statistical analysis: ***po0.0001 for viable vs dead, þ þ þpo0.0001 for VBNC vs dead; two-way ANOVA.
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