A cDNA Clone for Taxadiene Synthase, the Diterpene Cyclase That Catalyzes the Committed Step of Taxol Biosynthesis
Mark R. Wildung,Rodney Croteau +1 more
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TLDR
Sequence comparisons with monoterpene, sesquiterpenes, and diterpene cyclases of plant origin indicate a significant degree of similarity between these enzymes; the taxadiene synthase most closely resembles (46% identity, 67% similarity) abietadienes synthase, a diterPene cyclase from grand fir.About:
This article is published in Journal of Biological Chemistry.The article was published on 1996-04-19 and is currently open access. It has received 284 citations till now. The article focuses on the topics: Taxadiene & Taxadiene synthase.read more
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Plant terpenoid synthases: Molecular biology and phylogenetic analysis
TL;DR: This review focuses on the monoterpene, sesquiterpenes, and diterpene synthases of plant origin that use the corresponding C10, C15, and C20 prenyl diphosphates as substrates to generate the enormous diversity of carbon skeletons characteristic of the terpenoid family of natural products.
Journal ArticleDOI
The family of terpene synthases in plants: A mid-size family of genes for specialized metabolism that is highly diversified throughout the kingdom
TL;DR: The terpene synthases (TPSs) as mentioned in this paper are a family of enzymes responsible for the synthesis of various terpenes from two isomeric 5-carbon precursor molecules, leading to 5-carbinear isoprene, 10-carbon monoterpenes, 15-carbon sesquiterpenes and 20-carbenes.
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Structural Basis for Cyclic Terpene Biosynthesis by Tobacco 5-Epi-Aristolochene Synthase
Courtney M. Starks,Kyoungwhan Back,Kyoungwhan Back,Joseph Chappell,Joseph Chappell,Joseph P. Noel,Joseph P. Noel +6 more
TL;DR: These structures reveal an unexpected enzymatic mechanism for the synthesis of the bicyclic product, 5-epi-aristolochene, and provide a basis for understanding the stereochemical selectivity displayed by other cyclases in the biosynthesis of pharmacologically important cyclic terpenes.
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Genes, enzymes and chemicals of terpenoid diversity in the constitutive and induced defence of conifers against insects and pathogens.
TL;DR: This review examines what chemicals are produced, the genes and proteins involved in their biosynthesis, how they work, and how they are regulated and how insects and their associated pathogens interact with, elicit, and are affected by conifer-produced terpenoids.
References
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Book
Molecular Cloning: A Laboratory Manual
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
Book
Principles of Gene Manipulation
TL;DR: This book discusses Gene Manipulation: An All-Embracing Technique, which combines cutting and Joining DNA Molecules, and Applications of Recombinant DNA Technology, which aims to apply this technique to gene transfer to animals and plants.