A cell line derived from normal dog kidney (MDCK) exhibiting qualities of papillary adenocarcinoma and of renal tubular epithelium.
01 Nov 1970-Cancer (Wiley Subscription Services, Inc., A Wiley Company)-Vol. 26, Iss: 5, pp 1022-1028
TL;DR: Tissue culture cell line MDCK grew with the histologic characteristics of a papillary adenocarcinoma when cultured in vitro in a three‐dimensional matrix of collagen‐coated cellulose spongeor when inoculated in the chick embryo.
Abstract: Tissue culture cell line MDCK grew with the histologic characteristics of a papillary adenocarcinoma when cultured in vitro in a three-dimensional matrix of collagen-coated cellulose spongeor when inoculated in the chick embryo. When suspensions of cells were injected intravenously in the chicknumerous implants were seen in the brain one week later. Transplanted to the chorio-allantois with fragments of embryonic heartMDCK was found in the cardiac mesenchyme and within the lumens of many vessels of the transplanted heart. MDCK also exhibited the physiologic characteristics of renal tubular epithelium. Cultured on a glass surfaceMDCK formed a monolayer of epithelium interspersed with numerous blisterseach of which was composed of many cells. The walls of the blisters and the contiguous monolayers on glass were made up of polarized epithelium with microvilli on the medium-bathed surface. Time lapse movies demonstrated that the blisters were in active fluid transport. Low concentrations of ouabain abolished the blistering overnightsuggesting inhibition of an ATPase essential for the sodium-potassium pump associated with the plasma membrane.
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TL;DR: Systemes experimentaux pour etudier la biogenese de la surface cellulaire, organisation moleculaire de the surface de the cellule epitheliale, d'endocytose et transcytose, et insertion des proteines membranaires.
Abstract: Systemes experimentaux pour etudier la biogenese de la surface cellulaire. Organisation moleculaire de la surface de la cellule epitheliale. Biogenese des membranes apicales et basolaterales. Endocytose et transcytose. Insertion des proteines membranaires. Developpement de la polarite de surface
857 citations
TL;DR: Measurements of Na+ permeability of electrical and tracer methods ruled out exchange diffusion as a mechanism for ion permeation and the lack of current saturation in the I/deltapsi curves does not support the involvement of carriers.
Abstract: An epithelial cell line (MDCK) was used to prepare monolayers which, in vitro, develop properties of transporting epithelia. Monolayers were formed by plating cells at high densities (10(6) cells/cm2) on collagen-coated nylon cloth disks to saturate the area available for attachment, thus avoiding the need for cell division. An electrical resistance developed within 4-6 h after plating and achieved a steady-state value of 104 +/- 1.8 omega-cm2 after 24 h. Mature monolayers were morphologically and functionally polarized. They contained junctional complexes composed of desmosomes and tight junctions with properties similar to those of "leaky" epithelia. Monolayers were capable of maintaining a spontaneous electrical potential sensitive to amiloride, produced a net water flux from the apical to basal side, and discriminated between Na+ and Cl- ions. The MDCK permeability barrier behaves as a "thin" membrane with negatively charged sites. It has: (a) a linear conductance/concentration relationship; (b) an asymmetric instantaneous current/voltage relationship; (c) a reduced ability to discriminate between Na+ and Cl- caused by lowering the pH; and (d) a characteristic pattern of ionic selectivity which suggests that the negatively charged sites are highly hydrates and of medium field strength. Measurements of Na+ permeability of electrical and tracer methods ruled out exchange diffusion as a mechanism for ion permeation and the lack of current saturation in the I/deltapsi curves does not support the involvement of carriers. The discrimination between Na+ and Cl- was severely but reversibly decreased at low pH, suggesting that Na+-specific channels which exclude Cl- contain acidic groups dissociated at neutral pH. Bound Ca++ ions are involved in maintaining the integrity of the junctions in MDCK monolayers as was shown by a reversible drop of resistance and opening of the junctions in Ca++-free medium containing EGTA. Several other epithelial cell lines are capable of developing a significant resistance under the conditions used to obtain MDCK monolayers.
847 citations
Cites background from "A cell line derived from normal dog..."
...rators (50, 51, 1) have suggested that blisters...
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TL;DR: Evidence that fibroblast-derived soluble factors play a crucial role in the control of epithelial morphogenesis is obtained and the involvement of diffusible paracrine factors in morphogenetic epithelial-mesenchymal interactions is demonstrated and a strategy for their molecular characterization is provided.
512 citations
TL;DR: The transport kinetics of the influenza virus hemagglutinin from its site of synthesis to the apical plasma membrane of Madin-Darby canine kidney cells, a polarized epithelial cell line, were studied by a sensitive tryptic assay, demonstrating that the inhibition at low temperature was reversible.
492 citations
TL;DR: A shotgun-based lipidomics workflow was developed that enabled the absolute quantification of mammalian membrane lipidomes with minimal sample processing from low sample amounts and investigated the remodeling of the total cell membrane lipidome during the transition from a nonpolarized morphology to an epithelial morphology and vice versa.
Abstract: Tissue differentiation is an important process that involves major cellular membrane remodeling. We used Madin–Darby canine kidney cells as a model for epithelium formation and investigated the remodeling of the total cell membrane lipidome during the transition from a nonpolarized morphology to an epithelial morphology and vice versa. To achieve this, we developed a shotgun-based lipidomics workflow that enabled the absolute quantification of mammalian membrane lipidomes with minimal sample processing from low sample amounts. Epithelial morphogenesis was accompanied by a major shift from sphingomyelin to glycosphingolipid, together with an increase in plasmalogen, phosphatidylethanolamine, and cholesterol content, whereas the opposite changes took place during an epithelial-to-mesenchymal transition. Moreover, during polarization, the sphingolipids became longer, more saturated, and more hydroxylated as required to generate an apical membrane domain that serves as a protective barrier for the epithelial sheet.
426 citations
Cites background from "A cell line derived from normal dog..."
...Madin–Darby canine kidney (MDCK) cells are a cell culture model for epithelial polarization (3, 4)....
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References
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TL;DR: Afhnity for Monovalent Cations and Quantitative Relation between Effect of Na+ + K+ on Enzyme System and Active Transport in Intact Cell.
Abstract: Afhnity for Monovalent Cations. 597 Relationship of Enzyme System to ATP. 598 Isolation of Enzyme System from Other Cells with Active Transport of Na+ and K+ 602 Location of Enzyme System in the Cell. 604 Effect of Cardiac Glycosides. 605 Quantitative Relation between Effect of Na+ + K+ on Enzyme System and Active Transport in Intact Cell. 606 Nature of Enzyme System. 607 Relation of (Na + + K+)-Activated Enzyme System to Active Transport of Cations. 6 10 Conclusion. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6’4
1,946 citations
TL;DR: An established line of canine kidney cells (MDCK-USD) of male origin was shown to contain 3 metacentric chromosomes and possess a modal number of 81 and was susceptible to vaccinia, VSV and influenza (PR8) viruses.
Abstract: SummaryAn established line of canine kidney cells (MDCK-USD) of male origin was shown to contain 3 metacentric chromosomes and possess a modal number of 81. It grew rapidly in Eagle's MEM, displayed contact inhibition and was susceptible to vaccinia, VSV and influenza (PR8) viruses. Two additional MDCK lines, the parent line and another derivative from other laboratories, were compared to the USD line and could be distinguished on the basis of modal number and morphology of the metacentric chromosomes observed in each line.
339 citations
TL;DR: A cell line (MDCK) of dog kidney origin grows on a glass surface as a mosaic of epithelium with many multicellular hemispherical vesicles that produce brain metastases resembling adenocarcinoma.
Abstract: A cell line (MDCK) of dog kidney origin grows on a glass surface as a mosaic of epithelium with many multicellular hemispherical vesicles. The cells lining the blisters actively secrete into the cyst cavities. Suspensions of these cells injected intravenously in the chick embryo produce brain metastases resembling adenocarcinoma.
234 citations
TL;DR: Plaque assays on the MDCK-USD line and the parent M DCK line showed that the latter was more sensitive to A/Swine and A(2)/Japan 305 viruses, indicating that the cell culture system was as sensitive as the in ovo assay.
Abstract: A plaque assay system has been developed for types A and B influenza viruses in an established line of canine kidney cells (MDCK-USD). In addition to a homogeneous susceptible cell, consistent plaque production depends on the use of highly purified agar (Agarose). This quantitative system was used to determine the rate of adsorption, synthesis, and thermal inactivation of influenza viruses, as well as to determine a dose response curve. Plaque assays on the MDCK-USD line and the parent MDCK line showed that the latter was more sensitive to A/Swine and A(2)/Japan 305 viruses. Titration of standard virus pools in embryonated eggs and MDCK-USD indicated that the cell culture system was as sensitive as the in ovo assay.
231 citations
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