Figure 3. Chemogenetic activation of Gαq signaling and Ca2+ oscillations. (a) Schematic 2 illustration of the experimental setup. (b) Representative time-lapse confocal fluorescence images 3 of a HeLa cell coexpressing mNG-iK6DHFR-Gαq and R-GECO. Images were taken before and 4 after the addition of mDcTMP (5 µM). Scale bar, 10 μm. For the time-lapse movie, see Movie S3. 5 (c) The time course of mNG-iK6DHFR-Gαq translocation and Ca2+ spikes observed in the cell is 6 shown in panel b. The normalized fluorescence intensities of mNG-iK6DHFR-Gαq (bottom) and 7 R-GECO (top) in the cytoplasm were plotted as a function of time. (d) Heatmaps depicting 8 mDcTMP-induced Ca2+ oscillations for 17 randomly selected cells coexpressing mNG-iK6DHFR-9 Gαq and R-GECO. 10 11 12
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