Journal ArticleDOI
A colorimetric method for determining low concentrations of mercaptans.
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TLDR
A study of the specificity of the reaction indicates that it occurs only with the S − group (actual or potential, as in thiourea), and heavy-metal ions interfere with the reaction by irreversibly bonding any S − ions.About:
This article is published in Archives of Biochemistry and Biophysics.The article was published on 1958-04-01. It has received 1053 citations till now. The article focuses on the topics: Reactivity (chemistry) & Thiourea.read more
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Tissue sulfhydryl groups
TL;DR: A water-soluble (at pH 8) aromatic disulfide [5,5′-dithiobis(2-nitrobenzoic acid] has been synthesized and shown to be useful for determination of sulfhydryl groups.
Journal ArticleDOI
Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman's reagent
Jozef Sedlak,Raymond H. Lindsay +1 more
TL;DR: A simple spectrophotometric method for the routine concomitant determination of sulfhydryl groups in PB- SH, NP-SH, and T-SH fractions in various tissues is reported.
Book ChapterDOI
Reassessment of Ellman's reagent.
Journal ArticleDOI
Ellman's reagent: 5,5'-dithiobis(2-nitrobenzoic acid)--a reexamination.
TL;DR: The molar absorption coefficient of the dianion of TNB is 14,150 at 412 nm in dilute aqueous salt solutions and this value was confirmed independently by reduction of purified DTNB with cysteine.
Journal ArticleDOI
Determination of sulfhydryl groups with 2,2′- or 4,4′-dithiodipyridine☆
D.R. Grassetti,J.F. Murray +1 more
TL;DR: The proposed method is compared with known methods for the determination of sulfhydryl groups and permits determination of SH groups in simple compounds and in biological materials.
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Determination of sulfhydryl groups in certain biological substances.
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