A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases.

doi:10.1093/AJCP/28.1.56

Journal Article•DOI•

A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases.

Stanley Reitman¹, Sam Frankel¹•Institutions (1)

01 Jul 1957-American Journal of Clinical Pathology (AMERICAN JOURNAL OF CLINICAL PATHOLOGY)-Vol. 28, Iss: 1, pp 56-63

About: This article is published in American Journal of Clinical Pathology.The article was published on 1957-07-01. It has received 9424 citations till now.

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Nardostachys jatamansi protects against liver damage induced by thioacetamide in rats.

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Shakir Ali¹, Khursheed Ahmad Ansari¹, M.A Jafry¹, H Kabeer¹, G Diwakar¹ - Show less +1 more•Institutions (1)

Hamdard University¹

01 Aug 2000-Journal of Ethnopharmacology

TL;DR: A 50% ethanolic extract of the rhizomes of N. jatamansi is shown to possess hepatoprotective activity and Pretreatment of rats with the extract significantly ameliorated the liver damage in rats exposed to the hepatotoxic compound thioacetamide.

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78 citations

Journal Article•DOI•

Dietary Supplementation of Zinc Nanoparticles and Its Influence on Biology, Physiology and Immune Responses of the Freshwater Prawn, Macrobrachium rosenbergii

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Thirunavukkarasu Muralisankar¹, Periyakali Saravana Bhavan¹, S. Radhakrishnan¹, C. Seenivasan¹, N. Manickam¹, Veeran Srinivasan¹ - Show less +2 more•Institutions (1)

Bharathiar University¹

31 May 2014-Biological Trace Element Research

TL;DR: The study suggests that up to 60 mg ZnNPs kg−1 can be supplemented for regulating survival, growth and immunity of M. rosenbergii.

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Abstract: The present study was conducted to assess the influence of dietary zinc nanoparticles (size 50 nm) on the growth, biochemical constituents, enzymatic antioxidant levels and the nonspecific immune response of the freshwater prawn, Macrobrachium rosenbergii post larvae (PL). The concentrations of dietary supplement zinc nanoparticles (ZnNPs) were 0, 10, 20, 40, 60 and 80 mg kg(-1) with the basal diet, and the level of Zn in ZnNP-supplemented diets were 0.71, 10.61, 20.73, 40.73, 60.61 and 80.60 mg kg(-1), respectively. ZnNP-incorporated diets were fed to M. rosenbergii PL (initial body weight, 0.18 ± 0.02 g) in a triplicate experimental setup for a period of 90 days. ZnNP supplemented feed fed PL up to 60 mg kg(-1) showed significantly (P < 0.05) improved performance in survival, growth and activities of digestive enzymes (protease, amylase and lipase). The concentrations of biochemical constituents (total protein, total amino acid, total carbohydrate and total lipid), total haemocyte count and differential haemocyte count were elevated in 10-60 mg kg(-1) ZnNP supplemented feed fed PL. However, the PL fed with 80 mg ZnNPs kg(-1) showed negative results. Activities of enzymatic antioxidants [superoxide dismutase (SOD) and catalase (CAT)], metabolic enzymes [glutamate-oxaloacetate transaminase (GOT) and glutamate-pyruvate transaminase (GPT)] and the process of lipid peroxidation (LPO) in the hepatopancreas and muscle showed no significant alterations in 10-60 mg kg(-1) ZnNP supplemented feed fed PL. Whereas, 80 mg ZnNPs kg(-1) supplemented feed fed PL showed significant elevations in SOD, CAT, LPO, GOT and GPT. Therefore, 80 mg ZnNPs kg(-1) was found to be toxic to M. rosenbergii PL. Thus, the study suggests that up to 60 mg ZnNPs kg(-1) can be supplemented for regulating survival, growth and immunity of M. rosenbergii.

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78 citations

Cites methods from "A colorimetric method for the deter..."

...Activities of Metabolic Enzymes The metabolic enzymes such as glutamic oxaloacetate transaminase (GOT) and glutamic pyruvate transaminase (GPT) were analysed according to the method of Reitman and Frankel [26] using a media source kit (Medsource Ozone Biomedicals Pvt. Ltd. Haryana, India)....
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...The metabolic enzymes such as glutamic oxaloacetate transaminase (GOT) and glutamic pyruvate transaminase (GPT) were analysed according to the method of Reitman and Frankel [26] using a media source kit (Medsource Ozone Biomedicals Pvt....
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Journal Article•DOI•

Synergistic effect of silymarin and standardized extract of Phyllanthus amarus against CCl4-induced hepatotoxicity in Rattus norvegicus.

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Narayan Prasad Yadav¹, Anirban Pal¹, Karuna Shanker¹, D.U. Bawankule¹, Anil K. Gupta¹, Mahendra P. Darokar¹, Suman P. S. Khanuja¹ - Show less +3 more•Institutions (1)

Central Institute of Medicinal and Aromatic Plants¹

01 Dec 2008-Phytomedicine

TL;DR: The combination of sily marin and Phyllanthus amarus showed synergistic effect for hepatoprotection and silymarin with ethanolic extract of P. amarus showing better activity due to the higher concentration of phyllanthin in ethanoli extract in comparison to aqueous extract ofP.

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78 citations

Cites background or methods from "A colorimetric method for the deter..."

...serum glutamyl oxalacetic acid transaminase (SGOT), serum glutamyl pyruvate transaminase (SGPT) (Reitman and Frankel, 1957), alkaline phosphatase (ALKP) (Kind and King, 1954), serum bilirubin (SBLN) (Malloy and Evelyn, 1937), total protein and creatinine by using enzymatic reagents (Bayer Diagnostics, India)....
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...…analyzed for various biochemical parameters, i.e. serum glutamyl oxalacetic acid transaminase (SGOT), serum glutamyl pyruvate transaminase (SGPT) (Reitman and Frankel, 1957), alkaline phosphatase (ALKP) (Kind and King, 1954), serum bilirubin (SBLN) (Malloy and Evelyn, 1937), total protein and…...
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Journal Article•DOI•

Changes in oxidative metabolism in selected tissues of the crab (Scylla serrata) in response to cadmium toxicity.

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P. Sreenivasula Reddy¹, A. Bhagyalakshmi•Institutions (1)

Pondicherry University¹

01 Dec 1994-Ecotoxicology and Environmental Safety

TL;DR: The results suggest that Cadmium affects oxidative metabolism and induces hyperammonemia, and crabs switch over their metabolic profiles toward compensatory mechanisms for the survivability in cadmium-polluted habitats.

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78 citations

Journal Article•DOI•

In vivo radioprotection studies of 3,3′-diselenodipropionic acid, a selenocystine derivative

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Amit Kunwar¹, Punit Bansal, S. Jaya Kumar¹, P. P. Bag¹, P.J. Paul, Neetinkumar D. Reddy, Liladhar B. Kumbhare¹, Vikas Jain¹, R.C. Chaubey¹, Mazhuvancherry Kesavan Unnikrishnan, K.I. Priyadarsini¹ - Show less +7 more•Institutions (1)

Bhabha Atomic Research Centre¹

01 Feb 2010-Free Radical Biology and Medicine

TL;DR: It is confirmed that DSePA exhibits protective effects against whole-body gamma-radiation and the probable mechanisms of action involve the maintenance of antioxidant enzymes, prophylactic action through the attenuation of the DNA damage, and inhibition of apoptosis.

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78 citations

Cites methods from "A colorimetric method for the deter..."

...Blood samples were collected, serum was separated, and serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels were estimated by the reported method [27]....
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A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases.

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