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Journal ArticleDOI

A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases.

01 Jul 1957-American Journal of Clinical Pathology (AMERICAN JOURNAL OF CLINICAL PATHOLOGY)-Vol. 28, Iss: 1, pp 56-63
About: This article is published in American Journal of Clinical Pathology.The article was published on 1957-07-01. It has received 9424 citations till now.
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TL;DR: Oral pre-treatment with ethanolic extract of the roots of C. carandas showed significant hepatoprotective activity against CCl4 and paracetamol induced hepatotoxicity, and showed that ERCC possessed strong antioxidant activity, which may probably lead to the promising hepatoprotsective activities of C-carandas root extract.
Abstract: Oral pre-treatment with ethanolic extract of the roots of C. carandas (ERCC; 100, 200 and 400 mg/kg, po) showed significant hepatoprotective activity against CCl4 and paracetamol induced hepatotoxicity by decreasing the activities of serum marker enzymes, bilirubin and lipid peroxidation, and significant increase in the levels of uric acid, glutathione, super oxide dismutase, catalase and protein in a dose dependent manner, which was confirmed by the decrease in the total weight of the liver and histopathological examination. Data also showed that ERCC possessed strong antioxidant activity, which may probably lead to the promising hepatoprotective activities of C. carandas root extract. These findings therefore supported the traditional belief on hepatoprotective effect of the roots of C. carandas.

77 citations

Journal ArticleDOI
TL;DR: Results indicated that survival was higher in all dietary treatments as compared to the control fed fish, and mixture of these substances are promising as immune enhancher in aquacultured fish.
Abstract: The study investigated effect of dietary supplementation with malic acid, Bacillus subtilis and a mixture of the two (3 × 2 factorial trial) on Nile tilapia (Oreochromis niloticus) health. Treatment groups (T1–T6) were fed diets containing three levels of malic acid (0.0, 5.0 and 10.0 g/kg), each of which was supplemented with 0 and 1.1 × 105 cfu/g B. subtilis, respectively. Each treatment group was assigned randomly to triplicate groups of 35 fish (5.26 ± 0.06 g) in 18 tanks for 84 days. The results indicated that survival was higher in all dietary treatments as compared to the control fed fish. The highest values of FBW, WG and SGR (%), PER, PPV and ER were recorded in groups T4 and T6, which were fed diets supplemented with 5 g malic acid/kg and 1.1 × 105 cfu/g B. subtilis and 10 g malic acid/kg and 1.1 × 105 cfu/g B. subtilis, respectively. The best value of FCR was obtained in groups T4 and T6. The lowest total bacterial count in the gut and faeces was detected in fish from group T6. The values of haematocrit, haemoglobin, red blood cells, white blood cells, total protein, albumin and globulin were significantly higher (p < .05) in fish from groups T4 and T6 (diets supplemented with malic acid and B. subtilis). As compared to the control fed fish, te mixture of these substances are promising as immune enhancher in aquacultured fish.

77 citations


Cites methods from "A colorimetric method for the deter..."

  • ...Levels of serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were measured according to the method described by Reitman and Frankel (1957), and serum creatinine was measured with calorimetric and enzymatic determination methods as described by Henary, Cannon, and Winkleman…...

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Journal ArticleDOI
TL;DR: Ganoderma lucidum extract restored antioxidant defense and prevented hepatic damage consequent to the challenge by B[a]P, and possessed significant antimutagenic activity.

77 citations


Cites methods from "A colorimetric method for the deter..."

  • ...The extract prevented the increase of SGOT, SGPT, and ALP activities consequent to B[a]P challenge, and enhanced the levels f reduced glutathione (GSH) and activities of glutathione peroxidase (GPx), glutathione-S-transferase (GST), superoxide dismutase (SOD), and atalase (CAT)....

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  • ...Effect of Ganoderma lucidum extract on hepatic nzymes after B[a]P challenge The activity of SGOT, SGPT and ALP was significantly eleated in B[a]P treated group of animals....

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  • ...The effect of B[a]P on hepatic nzymes, such as serum glutamate oxaloacetate transaminase (GOT), glutamate pyruvate transaminase (GPT) and alkaline phosphtase (ALP), were lso evaluated....

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  • ...Serum was used for the deterination of glutamate pyruvate transaminase (GPT) (Reitman nd Frankel, 1957), glutamate oxaloacetate transaminase (GOT) Reitman and Frankel, 1957) and alkaline phosphatase activities ALP) (Kind and King, 1954)....

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Journal ArticleDOI
TL;DR: It is elucidated that the growth performance was not significantly changed by partial replacement of FM protein up to 50% by DBSFLM protein, which reduced the feeding cost by 15.6% as compared with the FM-control diet.

77 citations

Journal ArticleDOI
TL;DR: ECG's and serum levels of SGOT, LDH, and CPK were examined during the postoperative period in 50 patients with angina pectoris who had myocardial revascularization procedures to determine frequent complication of coronary artery surgery as determined by serial ECG's.

77 citations