A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases.
01 Jul 1957-American Journal of Clinical Pathology (AMERICAN JOURNAL OF CLINICAL PATHOLOGY)-Vol. 28, Iss: 1, pp 56-63
About: This article is published in American Journal of Clinical Pathology.The article was published on 1957-07-01. It has received 9424 citations till now.
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TL;DR: It could be concluded that HS and DS have potent protective effects against oxidative stress, lipid peroxidation, and DNA damage induced by AA toxicity in rats.
Abstract: Acrylamide (AA) is a heat-induced toxin formed during thermal processing of many commonly consumed foods, including meat products, French fries, potato crisps, bread, cereals, cookies, and coffee. There is thus potentially high dietary exposure of humans to AA, which can induce significant oxidative stress. Hesperidin (HS) and diosmin (DS) are flavone glycosides that have antioxidant properties. The aim of this study was to investigate the protective effects of HS and DS against AA toxicity. Fifty-six adult male Wistar albino rats were divided into seven groups. The first group was orally administered 0.5% (w/v) dimethyl sulfoxide (DMSO) and considered as the control group. The second and third groups were orally administered 10 mg/kg/day of HS or DS, respectively. The fourth group received 20 mg/kg/day of AA orally for 14 days. The fifth and sixth groups were given 10 mg/kg/day of HS or DS, respectively, followed by AA. The seventh group was given both HS and DS after AA administration. AA intoxication significantly (p ≤ 0.05) increased serum levels of liver function enzymes (ALT, AST, and ALP), kidney function products (urea and creatinine), oxidative DNA damage marker (OHdG), proinflammatory markers (TNF-α, IL-1β, and IL-6), lipid peroxidation marker (malondialdehyde), and nitric oxide (NO). On the other hand, it significantly (p ≤ 0.05) decreased levels of reduced glutathione (GSH) in the liver, kidney, and brain. The activities of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and catalase (CAT) in the liver, kidney, and brain tissues were also reduced. HS and DS supplementation prevented lipid peroxidation, normalized the serum parameters altered by AA, and enhanced the tissue concentrations and activities of antioxidant biomarkers. It could be concluded that HS and DS have potent protective effects against oxidative stress, lipid peroxidation, and DNA damage induced by AA toxicity in rats.
75 citations
Cites methods from "A colorimetric method for the deter..."
...To measure the serum levels of alanine transferase (ALT) and aspartate transferase (ALT), we used the methods illustrated by Reitman and Frankel (Reitman and Frankel 1957)....
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Journal Article•
TL;DR: Results revealed changes in plasma biochemistry suggested less hepatic and renal damages in animals receiving plant extracts along with fluorinated water in comparison to that receiving fluorinated drinking water alone.
Abstract: Aqueous extracts of T indica fruit pulp (100 mg/ kg body weight) and M oleifera seeds (50 mg/ kg body weight) orally once daily for 90 days lowered plasma fluoride concentrations in rabbits receiving fluorinated drinking water (200 mg NaF/ Liter water) Cortical indices and metaphysial width in animals receiving extracts also revealed beneficial effects of plant extracts Changes in plasma biochemistry suggested less hepatic and renal damages in animals receiving plant extracts along with fluorinated water in comparison to that receiving fluorinated water alone Preliminary results revealed these plant extracts have some potential to mitigate fluoride toxicity
75 citations
TL;DR: The results suggest that TBBP-A is capable of disturbing the heme metabolism in rats, with the most pronounced changes being noted with regard to indicators of porphyrogenic action.
75 citations
TL;DR: Methanol extract of the stem bark of P. marsupium possesses significant hepatoprotective activity, and the toxic effect of CCl4 was controlled significantly by restoration of the levels of serum bilirubin, protein and enzymes as compared to the normal and the standard drug silymarin-treated groups.
Abstract: OBJECTIVE : To evaluate the hepatoprotective activity of Pterocarpus marsupium stem bark extracts against carbon tetrachloride (CCl4)-induced hepatotoxicity. MATERIALS AND METHODS : Hepatotoxicity was induced in male Wistar rats by intraperitoneal injection of CCl4 (0.1 ml/kg/day for 10 days). Methanol and aqueous extracts of P. marsupium stem bark were administered to the experimental rats (25 mg/kg/day, p.o. for 14 days). The hepatoprotective effect of these extracts was evaluated by the assay of liver function biochemical parameters (total bilirubin, serum protein, alanine aminotransaminase, aspartate aminotransaminase, and alkaline phosphatase activities) and histopathological studies of the liver. RESULTS : In methanol extract-treated animals, the toxic effect of CCl4 was controlled significantly by restoration of the levels of serum bilirubin, protein and enzymes as compared to the normal and the standard drug silymarin-treated groups. Histology of the liver sections of the animals treated with the extracts showed the presence of normal hepatic cords, absence of necrosis and fatty infiltration, which further evidenced the hepatoprotective activity. CONCLUSION : Methanol extract of the stem bark of P. marsupium possesses significant hepatoprotective activity.
75 citations
Cites background from "A colorimetric method for the deter..."
..., total bilirubin,([13]) total protein,([14]) serum transaminases([15]) and serum alkaline phosphatase([16]) (Raichem, San Diego, CA 921111203)....
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TL;DR: It was demonstrated that a three-day pretreatment with either GL or GA exhibited protective effect on retrorsine-induced liver damage in rats, and extensive hepatocellular damages were observed.
75 citations