A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases.
01 Jul 1957-American Journal of Clinical Pathology (AMERICAN JOURNAL OF CLINICAL PATHOLOGY)-Vol. 28, Iss: 1, pp 56-63
About: This article is published in American Journal of Clinical Pathology.The article was published on 1957-07-01. It has received 9424 citations till now.
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TL;DR: It is revealed that Moringa oleifera has potent antioxidant and antigenotoxic actions against pendimethalin toxicity and could overcome the negative impacts of pendIMethalin and normalise the examined parameters nearly to the control values.
Abstract: To assess the ameliorative effects of Moringa oleifera (MO) leaf extract on haematological and biochemical changes, liver DNA damage and oxidative stress biomarkers in Nile tilapia (Oreochromis niloticus) exposed to a sublethal concentration (0.52 mg/l) of pendimethalin (PM). Tilapia fish were allocated into four equal groups in tri-replicates as follows: first group was the control group, second group was treated with MO (20 ml/30 l water), third group was exposed to 0.52 mg PM/l and fourth group was exposed to 0.52 mg PM/l and treated with MO leaf extract (20 ml/30 l water) for 28 days. At the end of this period, blood and liver tissue samples were collected and haematological and biochemical changes, hepatic DNA fragmentation and oxidative stress biomarkers were analysed. Pendimethalin caused significant reduction in haematological profile [White blood cells (WBCs) and red blood cells (RBCs) counts, haemoglobin (Hb) concentration and haematocrit (Ht) level]; meanwhile, serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), creatinine, uric acid, glucose, cortisol, cholesterol and lactate dehydrogenase (LDH) were significantly increased. On the other hand, serum total protein, albumin, globulin and acetylcholinesterase (AChE) were decreased. Significant reduction in hepatic superoxide dismutase (SOD), catalase (CAT), total antioxidant capacity (TAC) and glutathione peroxidase (GSH-Px) levels and marked increments of hepatic malondialdehyde (MDA) and DNA fragmentation were observed in PM-exposed fish compared to the control group. The addition of Moringa oleifera leaf extract into the water could overcome the negative impacts of pendimethalin and normalise the examined parameters nearly to the control values. Moringa oleifera was used for the first time to protect tilapia fish against PM-induced toxicity. The present study revealed that Moringa oleifera has potent antioxidant and antigenotoxic actions against pendimethalin toxicity.
64 citations
TL;DR: Signs of intoxication were found to be closely correlated with the accumulated dose of cadmium, and stop-flow analysis revealed proximal tubular dysfunction as well as glomerular and distal Tubular dysfunction.
64 citations
01 Jan 2008
TL;DR: The findings suggested that O.Stamineus methanol leaf extract possessed hepatoprotective activity, which brought back the altered levels of biochemical markers to the near normal levels in the dose dependent manner.
Abstract: The objective of this study was to investigate the hepatoprotective activity of Methanol extract of leaves of Orthosiphon stamineus against paracetamol induced hepatotoxicity. The material was dried in shade, they were powdered and Extracted with methanol. Preliminary phytochemical tests were done. Methanol extract showed presence of phenolic compound and flavanoids. The hepatoprotective activity of the methanol extract was assessed in paracetamol induced hepatotoxic Rats.Alteration in the levels of biochemical markers of hepatic damage like SGOT, SGPT, ALP and lipid peroxides were tested in both Paracetamol treated and untreaed groups. Paracetamol (2g/kg) has enhanced the SGOT, SGPT, ALP and the Lipid peroxides in liver. Treatment of methanolic extract of O.Stamineus leaves(200mg/kg)has brought back the altered levels of biochemical markers to the near normal levels in the dose dependent manner.Our findings suggested that O.Stamineus methanol leaf extract possessed hepatoprotective activity.
64 citations
TL;DR: The three enzymes, Na/K‐ATPase, glyceraldehyde‐3‐phosphate dehydrogenase and NADH‐ferricyanide reductase, were found not to change with age, but significant age‐dependent decreases were observed in the cases of acetylcholinesterase, phosphoglycerate kinase, purine nucleoside phosphorylase and alkaline phosphatase.
Abstract: Summary. Human erythrocytes from healthy male donors were fractionated with respect to in vivo age by simple centrifugation in order to characterize changes in the functional integrity of the membrane during the life-span of the cell. The three enzymes, Na/K-ATPase, glyceraldehyde-3-phosphate dehydrogenase and NADH-ferricyanide reductase, were found not to change with age, but significant age-dependent decreases were observed in the cases of acetylcholinesterase, phosphoglycerate kinase, purine nucleoside phosphorylase, adenylate kinase, Mg-ATPase and alkaline phosphatase.
The possibility mat these changes were attributable to mechanisms other than age-related inactivation, such as reticulocyte contamination, differential resealing and crypticity, was investigated. Only the decrease in acetylcholinesterase could be explained wholly in terms of reticulocyte contamination. A decrease in membrane integrity on ageing was observed, which accounted for approximately half the change in alkaline phosphatase and may have contributed to the other enzyme activity changes. This membrane integrity effect masked a real decrease in the highly cryptic NADH-ferricyanide reductase, this decrease being apparent only after total disaggregation of the membrane with nonionic surfactant.
64 citations
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...G O T was assayed by the method of Reitman & Frankel (1957) using a prepared kit purchased from BDH....
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Journal Article•
TL;DR: The results of the present study provide clear evidence that the ethanolic ZO extract pretreatment enhances the antioxidant defense against ISO-induced oxidative myocardial injury in rats and exhibit cardioprotective property.
Abstract: Ethanolic Z. officinale (ZO) extract (200 mg/kg) pretreatment for 20 days in isoproterenol (ISO)-treated rats significantly increased the levels of endogenous myocardial antioxidants (catalase, superoxide dismutase and tissue glutathione), decreased the levels of serum marker enzymes (lactate dehydrogenase, creatine kinase, aspartate transaminase and alanine transaminase) and increased myocardial lipid peroxides. Histological examination of rat's heart section confirmed myocardial injury with ISO administration and near normal pattern with ethanolic ZO extract pretreatment. The results of the present study, for the first time, provide clear evidence that the ethanolic ZO extract pretreatment enhances the antioxidant defense against ISO-induced oxidative myocardial injury in rats and exhibit cardioprotective property.
64 citations