A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases.
01 Jul 1957-American Journal of Clinical Pathology (AMERICAN JOURNAL OF CLINICAL PATHOLOGY)-Vol. 28, Iss: 1, pp 56-63
About: This article is published in American Journal of Clinical Pathology.The article was published on 1957-07-01. It has received 9424 citations till now.
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TL;DR: It is concluded that simultaneous supplementation of Aloe vera protects against arsenic induced oxidative stress but does not influence the arsenic concentration in these organs.
Abstract: Concomitant oral supplementation of Aloe vera, (1, 2 or 5% w[sol ]v in drinking water) during arsenic exposure (0.2 mg[sol ]kg, intraperitoneally, once daily for 3 weeks) was investigated in rats for its protective value. Animals exposed to arsenic (III) showed a significant inhibition of δ-aminolevulinic acid dehydratase (ALAD) activity, a marginal decrease in glutathione (GSH) and an increase in zinc protoporphyrin (ZPP) level in blood. White blood corpuscles (WBC) level decreased while most of the other clinical blood parameters like red blood cells count, haemoglobin, MCV, MCH, MCHC ratio and platelet number, etc. remained unaltered on arsenic exposure. Hepatic reduced GSH, oxidized glutathione (GSSG) level remained unaltered, thiobarbituric acid reactive substance (TBARS) level increased significantly while the activity of alkaline phosphatase (ALP), aspartate aminotransferase (AST), alanine aminotransferase (ALT) and catalase decreased on arsenic exposure. Renal GSH contents decreased while superoxide dismutase (SOD) activity decreased significantly on arsenic exposure. Concomitant administration of Aloe vera had remarkable protective action on inhibited blood ALAD activity and restored blood GSH level while most of the other blood biochemical parameters remained unchanged on Aloe vera supplementation. Interestingly, most of hepatic biochemical variables indicative of oxidative stress showed protection; no effect of Aloe vera on blood and liver arsenic concentration was noted. Also, no effect of Aloe vera on most of the altered renal biochemical parameters were noticed. The results thus lead us to conclude that simultaneous supplementation of Aloe vera protects against arsenic induced oxidative stress but does not influence the arsenic concentration in these organs. Copyright © 2005 John Wiley & Sons, Ltd.
63 citations
TL;DR: Nine of the 15 aqueous plant extracts tested exhibited greater than 25%—50% inhibition of hepatitis B‐virus (HBV) DNA polymerase activity, and also significantly stimulated production of the potent cytokine, tumour necrosis factor (TNF).
Abstract: The present study was undertaken to identify potential phytotherapeutic agents among the aqueous extracts of 151 herbal medicinal plants of Korea. Extracts were assayed for (1) binding potency to hepatitis B-virus surface antigen (HBsAg), and (2) inhibition of serum hepatitis B-virus DNA polymerase activity. Of the 151 aqueous plant extracts tested, 33 demonstrated a positive precipitation reaction with HBsAg + serum, and 15 of these specifically bound HBsAg in serum as well as recombinant HBsAg (Hepavax®). Nine extracts competitively inhibited >80% of anti-HBsAg antibody binding to Hepavax® HBsAg. Furthermore, nine of these 15 plant extracts also exhibited greater than 25%-50% inhibition of hepatitis B-virus (HBV) DNA polymerase activity, and also significantly stimulated production of the potent cytokine, tumour necrosis factor (TNF). None of the extract concentrations used altered liver function parameters of normal rats.
63 citations
TL;DR: Investigation of mushroom insoluble non-starch polysaccharides on carbon tetrachloride-induced hepatic damage in rat revealed that MINSP administration alone protected hepatocytes from the damage induced by CCl(4).
63 citations
Cites methods from "A colorimetric method for the deter..."
...Serum was prepared for biochemical analysis of aspartate and alanine aminotransferase (AST and ALT) activities according to the method of Reitman and Frankel (1957) using biomerieux diagnostic kits, France....
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TL;DR: Findings indicated that FN expression is an early and progressive event that occurs during liver fibrogenesis in vivo and in vitro.
Abstract: Increased fibronectin (FN) expression has an important role during liver fibrosis. The present study examined FN expression in rats subjected to carbon tetrachloride (CCl4)-induced liver fibrosis. In addition, the potential mechanisms underlying fibrogenesis were investigated by exposing hepatic stellate cells (HSCs) to transforming growth factor-β (TGF-β), which is a known inducer of myofibroblastic transformation of HSCs. Briefly, a rat model of liver fibrosis was created by administering intraperitoneal injections of CCl4. Furthermore, HSC-T6 cells were stimulated with increasing doses of recombinant TGF-β over 24 h. Hepatic fibrosis gradually increased following CCl4 administration in vivo. Western blotting and immunohistochemistry demonstrated that fibronectin (FN), TGF-β and α-smooth muscle actin (SMA) expression was increased following CCl4 injection, and the maximum expression levels were observed at 8 weeks. Once CCl4 treatment had been terminated, the expression levels of FN, TGF-β and α-SMA progressively declined to near baseline levels. Western blotting and quantitative polymerase chain reaction demonstrated that FN expression was gradually increased in response to TGF-β-stimulation of HSCs; maximum expression was achieved 12 h post-treatment (P<0.01 vs. the baseline). In conclusion, these findings indicated that FN expression is an early and progressive event that occurs during liver fibrogenesis in vivo and in vitro.
63 citations
Cites methods from "A colorimetric method for the deter..."
...Decreased serum ALT and AST activities, and TGF-β and α‑SMA protein levels were detected following the discontinuation of CCl4 treatment, mimicking the clinically observed expression patterns....
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...However, at 10 weeks (2 weeks after the final CCl4 injection), AST and ALT levels were markedly lower compared with those measured at 8 weeks (Fig....
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...Treatment with CCl4 (2‑8 weeks) resulted in a significant elevation in AST and ALT levels....
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...Serum aspartate transaminase (AST) and alanine transaminase (ALT) (15) activities were determined by the Clinical Laboratory (Beijing Friendship Hospital, Beijing, China)....
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TL;DR: Observations suggest that microsomal enzyme inducers, which increase the formation of the reactive metabolite, and fasting, which decreases the inactivation of the reaction by hepatic glutathione, may have additive effects on the hepatotoxicity of acetaminophen.
62 citations