A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases.
01 Jul 1957-American Journal of Clinical Pathology (AMERICAN JOURNAL OF CLINICAL PATHOLOGY)-Vol. 28, Iss: 1, pp 56-63
About: This article is published in American Journal of Clinical Pathology.The article was published on 1957-07-01. It has received 9424 citations till now.
Citations
More filters
TL;DR: The need to develop more sensitive techniques than double diffusion in agar for detecting the antigen and to investigate other agents than the Au(1) antigen is discussed in order to establish a screening procedure of carriers of hepatitis virus.
Abstract: . Nearly one fifth of patients transfused with blood containing Australia antigen(Au(1)-positive blood) produced antibodies against the Au(1) antigen. The antibodies were also found in recipients of Au(1)-netagive blood, although less frequently. A plasma product, heat-treated (60°C, 10 h) plasma protein solution, may also be an antigenic stimulus for antibody production. After transfusion the Au antigen became demonstrable in the serum of 18 of 55 recipients of Au(1)-positive blood and in the serum of 10 of 99 recipients of Au(1)-negative blood. Almost all of them developed hepatitis. In some patients, the antigen appeared before the elevation of SGPT level. The prevalence of post-transfusion hepatitis in the recipients of Au(1)-positive blood was higher than in the recipients of Au(1)-negative blood, and the difference was most significant between the groups who received less than five units of blood. The recipients who produced antibodies against the Au(1) antigen seemed less susceptible to the development of post-transfusion hepatitis. Since the majority of recipients of Au(1)-positive blood developed post-transfusion hepatitis, the transfusion of Au(1)-positive blood should be avoided. However, eliminating Au(1)-positive blood evidently will not completely prevent the occurrence of post-transfusion hepatitis. The need to develop more sensitive techniques than double diffusion in agar for detecting the antigen and to investigate other agents than the Au(1) antigen is discussed in order to establish a screening procedure of carriers of hepatitis virus.
59 citations
TL;DR: The effects of Leucaena leucocephala–Morus alba–Tectona grandis (2:1:1) based leaf meal mixture as a protein supplement to partially replace conventional supplements on the performance of female goats are investigated.
59 citations
TL;DR: Fish performance and feed intake were significantly enhanced with increasing ECBE levels, and its optimum level is 15 g/kg diet, and the dietary ECBE increased significantly the intestinal villi length/width and absorption area in a dose‐dependent manner.
Abstract: The occurrence of pathogenic bacteria, Aeromonas hydrophila, in farm‐raised fish requires urgent attention. Continuous and indiscriminate use of antibiotics as growth promoters and disease control agents in aquaculture have been discouraged because of the risk of development of antibiotic‐resistant bacterial strains. There is steady interest in the use of botanicals, such as clove, Eugenia caryophyllata, buds extract (ECBE), as alternatives. Hence, the present study evaluated the effect of dietary ECBE supplementation on the growth performance, physiological, antioxidant, and immunity biomarkers of African catfish, Clarias gariepinus. Fish (11.7 ± 0.5 g) were fed diets containing 0.0 (control), 5.0, 10.0, or 15.0 g ECBE/kg diet up to apparent satiation twice daily for 12 weeks. After the feeding trial, fish from each treatment were challenged with A. hydrophila infection by intraperitoneal injection and kept under observation for 14 days to record any abnormal clinical signs and daily mortality. The results demonstrated that fish performance and feed intake were significantly enhanced with increasing ECBE levels, and its optimum level is 15 g/kg diet. Further, the dietary ECBE increased significantly the intestinal villi length/width and absorption area in a dose‐dependent manner. There are significant progressive increases in the values of red blood cells, hemoglobin, hematocrit, white blood cells, platelets, lymphocytes, and heterocytes, while monocytes, eosinophil, and basophils decreased significantly due to dietary ECBE in a dose‐dependent manner. Highest glucose, cholesterol, total protein, globulin, and albumin‐globulin ratios, aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), urea, and creatinine values were found in fish fed 15 g ECBE/kg diets, while lowest values were recorded in fish fed the control diet. Despite the high AST and ALT values, no visible lesions or damage were observed in the liver cells of fish fed ECBE‐enriched diets. In addition, the inclusion of ECBE in fish diets enhanced the antioxidant and immunity capacity. Fish mortality after the bacterial challenge was higher in fish fed the control diet (82.3%) than those fed ECBE‐enriched diets. The lowest fish mortality was observed in fish fed the 15 ECBE/kg diet (4.7%) [Correction added on 16 November 2018: this section has been revised for clarity.].
59 citations
TL;DR: It is suggested that Chlorella vulgaris is effective for prevention of dyslipidemia which may be due to the modulation of lipid metabolism and increased fecal excretion of lipid.
Abstract: This study was performed to investigate effects of Chlorella vulgaris on lipid metabolism in rats fed high fat diet. Sixty 6-week-old male Wistar rats were divided into two groups; normal diet group and high fat diet group, then the rats in each group were further divided into three subgroups and fed 0%, 5% and 10% (w/w) chlorella-containing diets, respectively, and raised for 9 weeks. Serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activity and total protein and albumin concentration were not different among groups. Serum total lipids and liver TG concentration were significantly lower in 5% and 10% chlorella groups than 0% chlorella group in high fat diet groups (p<0.05). Serum TG, serum total cholesterol, liver total lipid and liver total cholesterol concentrations were significantly lower in 10% chlorella groups than 0% chlorella group in high fat diet groups (p<0.05). Fecal total lipid, TG and total cholesterol excretions were significantly higher in 5% and 10% chlorella groups than 0% chlorella groups in normal diet and high fat diet groups, respectively (p<0.05). These results suggest that Chlorella vulgaris is effective for prevention of dyslipidemia which may be due to the modulation of lipid metabolism and increased fecal excretion of lipid.
59 citations
Cites methods from "A colorimetric method for the deter..."
...Serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities were measured using a kit (Asan Pharmaceutical, Korea) based on the Reitman- Frankel method (Reitman & Frankel, 1957)....
[...]
TL;DR: This study supports the belief that the majority of viral hepatitis patients remain anicteric, as only 1 in 10 developed clinical jaundice.
59 citations