A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases.
01 Jul 1957-American Journal of Clinical Pathology (AMERICAN JOURNAL OF CLINICAL PATHOLOGY)-Vol. 28, Iss: 1, pp 56-63
About: This article is published in American Journal of Clinical Pathology.The article was published on 1957-07-01. It has received 9424 citations till now.
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TL;DR: The protective effect of tungsten is suggested to be limited to the conditions where the hepatic lesion is reported to be due to the generation of ROS, and the possible mechanism explaining the role of oxyanionic form of Tungsten in free radical-induced hepatic lesions is discussed.
57 citations
TL;DR: It was suggested that the extracts could protect the liver cells from paracetamolinduced liver damages perhaps by eliminating the deleterious effects of toxic metabolites from the drug.
Abstract: th day. The effect of the extracts on serum transaminase and alkaline phosphatase (ALP) were measured in the rats. The leaf, stem and root extracts of all the plants except Vitellaria paradoxa showed preponderance of saponin and tannin. Phlobatannin, cardiac glycosides and anthraquinones were observed in concentrations ranging between 10 to 100 mg/kg plant material. Stem bark extracts of the four plants produced significant (P < 0.05) hepatoprotective effects by decreasing the activity of serum enzymes. Values recorded for AST, ALT and ALP were significantly lower compared to those recorded for control rats. A higher inhibition of serum level elevation of ALP was observed with the four extracts. From these results, it was suggested that the extracts could protect the liver cells from paracetamolinduced liver damages perhaps by eliminating the deleterious effects of toxic metabolites from the drug.
57 citations
Cites methods from "A colorimetric method for the deter..."
...Assay for alanine transaminase (ALT) and aspartate transaminase (AST) were done by the method described by Reitman and Frankel (1957) and serum levels of alkaline phosphatase was measured by the method of Wright et al. (1970)....
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TL;DR: It is suggested that BB suppress DEN- induced hepatocarcinogenesis and could be developed as a promising chemopreventive natural supplement for liver cancer.
Abstract: Nitrosamine compounds are known hepatic carcinogens. This study was designed to study the efficacy of dietary supplementation with blueberries (BB) on diethylnitrosamine (DEN)-initiated hepatocarcinogenesis in male wistar rats. Rats were divided into three groups. The first group served as normal control group, the second group received DEN at a dose of 10 mg/kg body weight five times a week for 15 weeks. The third one received DEN as in DEN-treated group simultaneously with 4% BB-supplemented diet. The results showed that BB caused significant decrease in the elevated serum levels of α-fetoprotein (AFP), homocysteine (Hcy) along with levels of glutathione(GSH), deoxyribonucleic acid (DNA), ribonucleic acid (RNA)and activity of glutathione reductase (GR) in liver. Normalization of elevated α2-macroglobulin (α2M) and total antioxidant capacity (TAC) levels in serum, hepatic glutathione-S-transferase (GST), glutathione peroxidase (GPx) activities and liver weight was achieved whereas body weight was significantly decreased. Moreover, no significant change was observed in elevated relative liver weight, hepatic glucose-6-P-dehydrogenase (G6PD), lactate dehydrogenase (LDH) along with serum aminotransferases, alkaline phosphatase (ALP) and γ-glutamyltransferase (γ-GT) activities. Significant increase in reduced hepatic activity of xanthine oxidase (XO) was achieved and histopathological damage was minimized in BB-treated group. It is suggested that BB suppress DEN- induced hepatocarcinogenesis and could be developed as a promising chemopreventive natural supplement for liver cancer.
Key words: Blueberries, diethylnitrosamine, hepatocarcinogenesis, α2-macroglobulin, α-fetoprotein, homocysteine, glutathione, rats.
57 citations
Cites methods from "A colorimetric method for the deter..."
...Serum ALT and AST were assayed using commercially available kits provided by ProDia international, Germany, according to the method of Reitman and Frankel (1957)....
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TL;DR: Findings indicate that A. marmelos attenuates CCl4-mediated hepatic oxidative stress, toxicity, tumor promotion and subsequent cell proliferation response in Wistar rats, hence preventing further tissue damage, injury and hyperproliferation.
Abstract: The antioxidant properties and inhibitory effect on early tumor promoter markers of A. marmelos (25 and 50 mg/Kg b. wt. orally) have been evaluated. Male Wistar rats were pre-treated for seven consecutive days with A. marmelos prior to CCl4 (1 mL Kg(- 1) body weight p. o., in corn oil [1:1 v/v]) treatment. Pre-treatment with A. marmelos suppressed lipid peroxidation (LPO), xanthine oxidase (XO) and release of serum toxicity marker enzymes viz, SGOT, LDH, SGPT dose-dependently and significantly (p < 0.001). Hepatic antioxidant status viz, reduced glutathione (GSH), glutathione reductase (GR), glutathione peroxidase (GPx), quinone reductase (QR), catalase (CAT) were concomitantly restored in A. marmelos-treated groups (p < 0.001). In addition, A. marmelos pretreatment also prevented the CCl4-enhanced ornithine decarboxylase (ODC) and hepatic DNA synthesis significantly (p < 0.001). In conclusion, carbon tetrachloride-induced liver toxicity was strikingly attenuated by A. marmelos treatment and the study gives some insight into the mechanisms involved in diminution of free radical generating toxicants and enhancement of the antioxidant armory, hence preventing further tissue damage, injury and hyperproliferation. Thus, these findings indicate that A. marmelos attenuates CCl4-mediated hepatic oxidative stress, toxicity, tumor promotion and subsequent cell proliferation response in Wistar rats.
57 citations
TL;DR: Restoration of miR-145 levels may serve as a promising therapeutic strategy for management of venous thrombosis and identifies a previously undescribed role of miRNA in VT by regulating TF expression.
57 citations
Cites methods from "A colorimetric method for the deter..."
...Circulating biomarkers for liver and heart functionwere determined in serum samples subsequently analyzed for alanine and aspartate aminotransferases (ALT & AST) using colorimetric measurement of hydrazone formed with 2, 4 dinitrophenyl hydrazine (Reitman and Frankel, 1957), alkaline phosphatase (ALP) and lactate dehydrogenase (LDH) levels by the phenolphthalein monophosphate method (Babson and Phillips, 1965)....
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...…for alanine and aspartate aminotransferases (ALT & AST) using colorimetric measurement of hydrazone formed with 2, 4 dinitrophenyl hydrazine (Reitman and Frankel, 1957), alkaline phosphatase (ALP) and lactate dehydrogenase (LDH) levels by the phenolphthalein monophosphate method (Babson and…...
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