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Journal ArticleDOI

A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases.

01 Jul 1957-American Journal of Clinical Pathology (AMERICAN JOURNAL OF CLINICAL PATHOLOGY)-Vol. 28, Iss: 1, pp 56-63
About: This article is published in American Journal of Clinical Pathology.The article was published on 1957-07-01. It has received 9424 citations till now.
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TL;DR: It is shown that OVEO is an effective counteractive treatment against CP-induced damage in exposed common carp and is recommended during the formulation of fish rations.

56 citations

Journal ArticleDOI
TL;DR: It is inferred that the duration and magnitude of stress applied in the present experiments did not cause major permanent alterations in the rice cultivar ‘Kranti’, and the significant basic information gathered in such experiments, particularly on recovery potential, can be utilised for varietal screening.
Abstract: In laboratory experiments, rice plants cv ‘Kranti’ were stressed osmotically using polyethylene glycol ‘6000’ and mannitol, whereas, in pots, plants were drought stressed by withholding water supply. Both osmotic and drought stress influenced different aspects of nitrogen metabolism, resulting in a decline in the activities of aspartate aminotransferase, alanine amino-transferase and an increase in protease activity accompanied by increased free proline and alterations in other amino acid content. An influence on α-amylase activity, total free sugars and starch contents was also observed, reflecting the impact of water stress on interconversion between starch and simpler sugars. Effects of polyethylene glycol ‘6000’ as an osmotic agent were more consistent than those of mannitol during short-term (18 h) stress imposition, probably because of slight absorption of mannitol. Since the recovery for most of the parameters was substantial on release of stress, one can infer that the duration and magnitude of stress applied in the present experiments did not cause major permanent alterations in the rice cultivar ‘Kranti.’ The significant basic information gathered in such experiments, particularly on recovery potential, can be utilised for varietal screening.

56 citations


Cites methods from "A colorimetric method for the deter..."

  • ...For ‘stress release’ seedlings/plants were transferred to ‘control’ conditions for 18 h. Aspartate and alanine amino-transferases were assayed following the method of Reitman and Frankel (1957)....

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Journal ArticleDOI
TL;DR: Excerpt Persistentobstructive jaundice produced by pancreatitis is a well-documented phenomenon but in patients without demonstrable extrahepatic biliary tract disease, often alcoholics, the ja...
Abstract: Excerpt Persistentobstructive jaundice produced by pancreatitis is a well-documented phenomenon (1-10). In patients without demonstrable extrahepatic biliary tract disease, often alcoholics, the ja...

56 citations

Journal ArticleDOI
TL;DR: Evaluation of the effectiveness of L‐ascorbic acid (AA) in alleviating the toxicity of aflatoxin B1 (AFB1) in male New‐Zealand white rabbits showed that the presence of AA could diminished the adverse effects of AFB1 on most of hematological and biochemical values, and enzymatic activities in rabbits.
Abstract: This study was undertaken to evaluate the effectiveness of L-ascorbic acid (AA) in alleviating the toxicity of aflatoxin B1 (AFB1) in male New-Zealand white rabbits. Five rabbits (6 months of age and mean body weight 3.12 kg) per group were assigned to 1 of 6 treatment groups: 0 mg AA and 0 mg AFB1/kg BW (control); 20 mg AA/kg BW; 15 microg AFB1/kg BW; 15 microg AFB1 plus 20 mg AA/kg BW; 30 pg AFB1/kg BW; 30 pg AFB1 plus 20 mg AA/kg BW. Rabbits were orally administered their respective doses every other day for 9 weeks, followed by a 9-week recovery period where all drugs were withdrawn. Evaluations were made for hemato-biochemical parameters and enzymatic activities. Results showed that AFB1 significantly (p < 0.05) decreased hemoglobin (Hb), total erythrocytic count (TEC) and packed cell volume (PCV), in a dose-dependent manner, and these effects were continued during the recovery period. Ascorbic acid caused an increase in these parameters, and alleviated the negative effect of AFB1 during the treatment period. Additionally, serum concentrations of total protein, albumin and glucose were significantly (P < 0.05) declined by treatment with the high dose of aflatoxin and these effects were continued during the recovery period. Ascorbic acid caused non-significant increases in these parameters and alleviated the harmful effect of AFB1. On the other hand, aflatoxin treatment caused significant increases (P < 0.05) in the activities of serum aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (AlP) during the treatment period in a dose dependent manner, and this effect was continued during the recovery period, especially with the high dose. Also, treatment with the high dose of aflatoxin caused significant increases (P<0.05) in cholesterol and total bilirubin. Ascorbic acid caused significant decreases in these parameters and alleviated the harmful effects of AFB1. Whereas, Total leukocyte count (TLC), urea and creatinine were not significantly affected by aflatoxin-treatment. Generally, it is interesting feature that the treatment with AA alone had no negative effects on most of the previous parameters. Also, the presence of AA could diminished the adverse effects of AFB1 on most of hematological and biochemical values, and enzymatic activities in rabbits.

56 citations


Cites methods from "A colorimetric method for the deter..."

  • ...[20] The activities of serum aspartate aminotransferase (AST), alanine aminotransferase (ALT) were assayed by the method of Reitman & Frankel....

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  • ...The activities of serum aspartate aminotransferase (AST), alanine aminotransferase (ALT) were assayed by the method of Reitman & Frankel.([21]) Alkaline phosphatase (ALP) activity was determined according to the method of Kind and King....

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Journal ArticleDOI
TL;DR: Chrysin is a flavonoid that exists in nature and is the major component of some traditional medicinal herbs and exhibits hepatoprotective and antihyperlipidaemic activity and Rats treated with chrysin at different concentrations caused a significant improvement in serum protein level, decreased hepato and nephro toxicity markers.

56 citations