A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases.
01 Jul 1957-American Journal of Clinical Pathology (AMERICAN JOURNAL OF CLINICAL PATHOLOGY)-Vol. 28, Iss: 1, pp 56-63
About: This article is published in American Journal of Clinical Pathology.The article was published on 1957-07-01. It has received 9424 citations till now.
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TL;DR: Findings demonstrate that the phenolic constituents of Ficus carica leaf and Morus alba root bark are responsible at least in part for the observed protective effects.
Abstract: Context: In the absence of reliable liver-protective drugs in modern medicine, a large number of medicinal preparations are recommended for treatment of liver disorders.Objective: The antioxidant, hepatoprotective and kidney protective activities of methanol extracts of Ficus carica Linn. (Moraceae) leaves and fruits and Morus alba Linn. root barks (Moraceae) are evaluated here.Materials and methods: Liver and kidney damage were induced in rats by carbon tetrachloride in a subcutaneous dose of 1 mL (40% v/v in corn oil)/kg. The extract was given intraperitoneally at doses of 50 mg/kg (F. carica leaf and M. alba root bark) and 150 mg/kg (F. carica fruit). The activity of the extracts was comparable to that of silymarin, a known hepatoprotective agent. Antioxidant activity was evaluated by measuring blood glutathione (GSH) content, superoxide dismutase (SOD), catalase (CAT) activities, and malondialdehyde equivalent (MDA). Hepatoprotective activity was evaluated by measuring serum levels of aspartate aminot...
54 citations
Cites methods from "A colorimetric method for the deter..."
...…colorimetrically as described by Yoshioka et al. (1979), AST and ALT activity in plasma were determined by a colorimetric method as described by Reitman and Frankel (1957), ALP level was determined by a kinetic method according to the method of Henry (1964), serum total protein was determined…...
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TL;DR: The results suggest that biliary LDH can be used for assessing injury of BEC in cold-preserved livers and that normothermic reperfusion does not aggravate preservation-induced injury ofBEC after cold ischemic storage.
54 citations
TL;DR: Liver protection against t-BHP induced hepatic injury was evident by a significant decrease in serum enzymes marker, sleeping time and MDA and an increase in the GSH, SOD and CAT activities confirmed by pathology tests, and possible mechanism of this protection may be associated with its property of scavenging free radicals which may be due to the presence of phenolic compounds.
54 citations
TL;DR: Investigation of the cardioprotective effect of sesamol on isoproterenol (ISO)-induced myocardial infarction in adult male albino Wistar rats found the 50 mg dose was more effective than the other two doses, and Histopathological observations correlated with the biochemical parameters.
Abstract: This study was designed to investigate the cardioprotective effect of sesamol on isoproterenol (ISO)-induced myocardial infarction in adult male albino Wistar rats. The heart damage induced by ISO was indicated by elevated levels of the marker enzymes such as alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), creatine kinase (CK), creatine kinase-MB (CK-MB) and the levels of troponin T and I in the plasma. In addition, lipid peroxidative markers such as thiobarbituric acid reactive substances (TBARS), conjugated dienes (CD) and lipid hydroperoxides (LHP) significantly increased in the plasma and heart. Activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione-S-transferase (GST) and glutathione reductase (GR) significantly decreased in the heart and (non-enzymic antioxidants) vitamin C, vitamin E and reduced glutathione (GSH)) levels significantly decreased in the plasma and heart in ISO-rats. Histopathological o...
53 citations
TL;DR: The results confirmed that coadministration of the tested extracts or silymarin with CCl(4) for 4 wk exhibited a marked hepatoprotective activity, attributed to their antioxidant potential, membrane stabilizing effect, and antifibrogenic activities.
Abstract: The present study was designed to investigate the bioactive compounds in extracts of Rumex vesicarius L. (Polygonaceae), a wild edible herb growing in Egypt. Ethyl acetate and n-butanol fractions of leaves of R. vesicarius were conducted by HPLC-PDA-MS/MS-ESI in the negative mode to analyze phenolics content. Results revealed the identification of 13 phenolic compounds: 8-C-glucosyl-apigenin, 8-C-glucosyl-luteolin, 6-C-hexosyl-quercetin, 3-O-rutinosyl-quercetin, 7-O-rhamno-hexosyl-diosmetin, 7-O-rhamno-acetylhexosyl-diosmetin, catechin, epicatechin, ferulohexoside, 6-C-glucosyl-naringenin, epicatechin gallate, 6-C-glucosyl-catechin, and epigallocatechin gallate. Quantification of the identified compounds revealed that 6-C-glucosyl-naringenin was the major compound. Also, qualitative and quantitative analysis of the hydro-ethanolic extract of leaves was carried out for ascorbic acid, α-tocopherol, β-carotene. The essential oil as well as lipids analysis of saponifiable and unsaponifiable matters. The biochemical studies were conducted to evaluate the antioxidant and hepatoprotective potential of roots (REE), leaves (LEE), and fruits (FEE) ethanolic extracts of R. vesicarius (100 mg/kg b.wt., p.o., each) against hepatotoxicity induced by CCl4 (0.5 mL/kg b.wt., p.o., 3 times a week) compared with silymarin (50 mg/kg b.wt., p.o.) as standard drug. The results confirmed that coadministration of the tested extracts or silymarin with CCl4 for 4 wk exhibited a marked hepatoprotective activity, attributed to their antioxidant potential, membrane stabilizing effect, and antifibrogenic activities.
Practical Application: Investigation of the effect of hommad as a safe hepatoprotective diet that prospectively directs the attention to a valuable therapeutic natural herb rich in bioactive constituents.
53 citations