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Journal ArticleDOI

A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases.

01 Jul 1957-American Journal of Clinical Pathology (AMERICAN JOURNAL OF CLINICAL PATHOLOGY)-Vol. 28, Iss: 1, pp 56-63
About: This article is published in American Journal of Clinical Pathology.The article was published on 1957-07-01. It has received 9424 citations till now.
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Journal ArticleDOI
TL;DR: Results indicate that 3-ASP at a dose range of 5-50 mg/kg was especially potent and produced systemic anti-hyperalgesic and antinociceptive actions in mice.
Abstract: In this study, antinociceptive and anti-hyperalgesic effects of 3-alkynyl selenophene (3-ASP) were evaluated in mice. Acute toxicity of 3-ASP (1–50 mg/kg, per oral) was investigated in mice. 3-ASP neither caused toxicity nor affects locomotor activity in the rota-rod test. 3-ASP did not change plasma aspartate (AST) and alanine aminotransferase (ALT) activities, urea and creatinine levels. 3-ASP caused a significant increase in tail-immersion and hot-plate response latencies time. 3-ASP inhibited early and late phases of nociception caused by intraplantar (i.pl.) injection of formalin. 3-ASP reduced nociception produced by i.pl. injection of glutamate, bradykinin, phorbol myristate acetate (PMA) and capsaicin in mice. Mechanical hyperalgesia induced by Freund's Complete Adjuvant (CFA) was attenuated by 3-ASP administration to mice (maximal inhibition of 42 ± 11%). The anti-hyperalgesic effect of 3-ASP was maintained for up to 6 h. The antinociceptive effect of 3-ASP was not abolished by naloxone (5 mg/kg), discarding the involvement of opioidergic mechanism in this effect. These results indicate that 3-ASP at a dose range of 5–50 mg/kg was especially potent and produced systemic anti-hyperalgesic and antinociceptive actions in mice.

53 citations


Cites methods from "A colorimetric method for the deter..."

  • ...Plasma aspartate (AST) and alanine aminotransferase (ALT) activities, used as the biochemical markers for the early acute hepatic damage, were determined by the colorimetric method of Reitman and Frankel (1957)....

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Journal ArticleDOI
TL;DR: It is suggested that phospholipid complex of hesperetin produced better antioxidant activity than free drug at the same dose level and the effect persisted for a longer period of time, which may be helpful in solving the problems of faster elimination of the molecule.
Abstract: Hesperetin is known to exhibit a variety of pharmacological activities in mammalian cell systems. Although it shows appreciable bioavailability when administered orally, its faster elimination from body creates the need of frequent administration to maintain effective plasma concentration. To overcome this limitation, a phospholipid complex of hesperetin was prepared and evaluated for antioxidant activity and pharmacokinetic profile. The hesperetin content of the complex was determined by a spectrophotometer and the surface characteristics of the complex were studied by means of microscope. The antioxidant activity was evaluated in carbon-tetrachloride-intoxicated rats at a dose level of 100 mg/kg body weight, p.o. The complex was studied for in vitro drug release characteristics and effect of complexation on serum concentration of hesperetin in rats was also studied along with main pharmacokinetic parameters. The results showed that the complex has a sustained release property and enhanced antioxidant activity (P < 0.05 and <0.01) as compared to free hesperetin at the same dose level. Pharmacokinetic study depicted that the complex has higher relative bioavailability and acted for a longer period of time. The study therefore suggests that phospholipid complex of hesperetin produced better antioxidant activity than free drug at the same dose level and the effect persisted for a longer period of time, which may be helpful in solving the problems of faster elimination of the molecule.

53 citations


Cites methods from "A colorimetric method for the deter..."

  • ...From the liver function tests and the antioxidant activity studies, it is observed that pretreatment with pure hesperetin (100 mg/kg) on alternate days neither reduced the elevated levels of SGOT, SGPT, SALP, total bilirubin, and TBARS nor increased the depleted levels of GPX, SOD, and CAT....

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  • ...Hepatic damage induced by CCl4 caused significant rise in marker enzymes SGPT, SGOT, SALP, and also in serum bilirubin....

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  • ...Serum was separated by centrifugation and then liver function tests were performed by measuring serum glutamate oxaloacetate transaminase (SGOT) and serum glutamate pyruvate transaminase (SGPT) (23)....

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Journal ArticleDOI
TL;DR: The vitamin B6 metabolism becomes altered with higher ages, and a defect in absorption or in phosphorylation of the vitamin is thought to be responsible.

53 citations

Journal Article
TL;DR: The results when compared with the standard drug silymarin revealed that the hepatoprotective activity of the constituent ursolic acid is significant as similar to the standardDrug.
Abstract: The ethanol extract of C. serratum roots and ursolic acid isolated from it were evaluated for hepatoprotective activity against carbon tetrachloride induced toxicity in male Wistar strain rats. The parameters studied were estimation of liver function serum markers such as serum total bilirubin, total protein, alanine transaminase, aspartate transaminase and alkaline phosphatase activities. The ursolic acid showed more significant hepatoprotective activity than crude extract. The histological profile of the liver tissue of the root extract and ursolic acid treated animal showed the presence of normal hepatic cords, absence of necrosis and fatty infiltration as similar to the controls. The results when compared with the standard drug silymarin, revealed that the hepatoprotective activity of the constituent ursolic acid is significant as similar to the standard drug.

53 citations

Journal ArticleDOI
TL;DR: The increment in LDH and ALP in milk of udders shows the presence of tissue damage provoked by SCM, and these parameters might be suitable for use in the early diagnosis of subclinical mastitis in ewes.

53 citations