A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases.
01 Jul 1957-American Journal of Clinical Pathology (AMERICAN JOURNAL OF CLINICAL PATHOLOGY)-Vol. 28, Iss: 1, pp 56-63
About: This article is published in American Journal of Clinical Pathology.The article was published on 1957-07-01. It has received 9424 citations till now.
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TL;DR: The frequency of somaclonal variations was found to be age dependent and similar isoenzyme patterns for PER and GOT were detected in all analyzed plants, but variations in activities of the three enzymes studied and in POD isoenzymes were detected.
Abstract: Isoenzyme analysis and activities of peroxidase (PER), polyphenol oxidase (POD) and glutamate oxaloacetate (GOT) and randomly amplified polymorphic DNA (RAPD) fingerprints were used to analyze somaclonal variations in tissue culture-derived date palm plants. The frequency of somaclonal variations was found to be age dependent. Similar isoenzyme patterns for PER and GOT were detected in all analyzed plants. However, variations in activities of the three enzymes studied and in POD isoenzymes were detected. RAPD analysis showed genetic variations in approximately 4 % of the analyzed plants (70 regenerants). The genetic variations were only detected in 6- and 12-months-old cultures. It was observed also that all morphologically abnormal shoots showed genetic variations at the molecular level.
107 citations
TL;DR: It is demonstrated that serum ferritin levels together with TIBC saturation levels are adequate methods for screening populations and conclude that idiopathic hemochromatosis is not as rare as previously thought.
Abstract: An increase in the iron content of food may be harmful to people with genetic hemochromatosis. We studied the prevalence of this disorder in Sweden, which is the country with the world's highest iron fortification of food. Serum ferritin and transferrin (TIBC) saturation levels were used as initial screening methods. Three (0.5%) of 623 males aged 30-39 years were found to have genetic hemochromatosis. Family studies revealed 10 additional homozygotic family members. A prevalence of 0.5% of homozygotes (q2) implies a gene frequency (q) of 6.9% or a heterozygote frequency (2 x Q) of 13.8%. The high gene frequency may be explained by a possible genetic advantage of heterozygotes in the past. We conclude that idiopathic hemochromatosis is not as rare as previously thought. Affected persons should be detected and treated before irreversible organ damage occurs. This study demonstrates that serum ferritin levels together with TIBC saturation levels are adequate methods for screening populations.
107 citations
TL;DR: Results indicate that DEP brings about significant changes in the activity of certain liver and muscle enzymes, which may have long-term effects on fish that are continuously exposed to low doses of DEP in the aquatic environment.
107 citations
TL;DR: Spirulina supplementation could overcome DLM-induced hepatotoxicty, nephroprotective, neuroprotective and neurotoxicity by abolishing oxidative tissue injuries in a dose-dependent manner.
107 citations
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...Serum aminotransferases, AST and ALT were evaluated according to [28], Alkaline phosphatase (ALP) according to [29]....
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TL;DR: Histopathologic study revealed liver damage following continuous exposures to high levels of dichlorodifluoromethane, and to lower levels of carbon tetrachloride, and 1,1-dichloroethylene (189 mg/m3).
107 citations