A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases.
01 Jul 1957-American Journal of Clinical Pathology (AMERICAN JOURNAL OF CLINICAL PATHOLOGY)-Vol. 28, Iss: 1, pp 56-63
About: This article is published in American Journal of Clinical Pathology.The article was published on 1957-07-01. It has received 9424 citations till now.
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TL;DR: TQ is effective in protecting mice against acetaminophen-induced hepatotoxicity possibly via increased resistance to oxidative and nitrosative stress as well as its ability to improve the mitochondrial energy production.
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...Serum alanine aminotransferase (ALT) activity was determined according to the method of Reitman and Frankel (1957)....
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TL;DR: Results suggest that methanolic extract of V. amygdalina leaves posseses protective effect against CCl(4)-induced hepatotoxicity by the antioxidant mechanism of action.
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TL;DR: By analysing this mitochondrial fraction on discontinous sucrose gradients, the authors showed the localization of both enzymes to be different, suppose GABAT to be localized in mitochondria and GAD in nerve-endings.
Abstract: IN studying the intracellular localization of glutamate decarboxylase (GAD, E.C. 4.1.1.15) in brain tissue of the rat, LDVTRUP (1961) and HILGERSOM (1962) could not detect any considerable amount of this enzyme in the mitochondrial fraction. SALGANICOFF and DE ROBERTIS (1963) prepared a mitochondrial fraction containing approximately 40 per cent of the GAD and 70-80 per cent of the y-aminobutyrate transaminase (GABAT, E.C. 2.6. I.-). By analysing this mitochondrial fraction on discontinous sucrose gradients, these authors showed the localization of both enzymes to be different. They suppose GABAT to be localized in mitochondria and GAD in nerve-endings. In view of the conflicting data on the intracellular localization of GAD we thought it desirable to reinvestigate this problem, including GABAT in the the analysis. The mitochondrial fraction was further studied by using centrifugation on continuous sucrose gradients, this technique being more refined than those used by DE ROBERTIS, PELLEGRINO DE IRALDI, RODRIGUEZ DE LORES ARNAIZ and SALGANICOFF (1 962) and WHITTAKER (1959). In all of the fractions cytochrome oxidase (E.C. 1.9.3.1), aspartate transaminase (GOT, E.C. 2.6.1. l), alanine transaminase (GPT, E.C. 2.6.1.2), lactate dehydrogenase (LDH, E.C. 1.1.1.27), alkaline phosphatase (E.C. 3.1.3.1), acid phosphatase (E.C. 3.1.3.2) and protein, were measured. and H. VELDSTRA*
106 citations
TL;DR: It was suggested that the hepatoprotective effects of the LFE might be related to antioxidative activity and expressional regulation of CYP2E1.
106 citations
TL;DR: Chlorogenic acid isolated from Anthocephalus cadamba exhibited a better therapeutic protective action than silymarin (SM), in CCl4‐administered mice, revealing that the antioxidative action of CGA is responsible for its liver protective activity.
Abstract: In the present study, chlorogenic acid (CGA) isolated from Anthocepha cadamba was screened for hepatoprotective activity by in vitro and in vivo assay methods using carbon tetrachloride (CCl 4 ) as a model of liver injury. Intraperitoneal administration of CGA to mice at a dose of 100 mg/kg body weight for 8 days caused significant reversal in lipid peroxidation, enzymatic leakage, cytochrome P45O (Cyt P45O) inactivation and produced enhancement of cellular antioxidant defence in CCl 4 -intoxicated mice, revealing that the antioxidative action of CGA is responsible for its liver protective activity. CGA exhibited a better therapeutic protective action than silymarin (SM), in CCl 4 -administered mice
106 citations